Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması
Yıl: 2019 Cilt: 35 Sayı: 3 Sayfa Aralığı: 131 - 138 Metin Dili: Türkçe DOI: 10.15312/EurasianJVetSci.2019.236 İndeks Tarihi: 01-04-2020
Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması
Öz: Amaç: Bu çalışmada FHV (Feline Herpesvirus) ve FCV (Feline Calicivirus) şüpheli örneklerde söz konusu enfeksiyonların varlığı/yaygınlığının araştırılması, bildirilen semptomlarlailişkilendirilmesi ve bu virusların moleküler karakterizasyonu amaçlandı.Gereç ve Yöntem: Bu amaçla klinik olarak solunum sistemi problemi gösteren toplam 70 kediden 31 nasal, 30 konjunktival, 8 oral, 7 orafarengeal ve 11 rektal swap ile 32 EDTA’lı kan örneği olmak üzere toplam 119 örnek alınmıştır. Alınan örneklerden viral nükleik asit ekstraksiyonu yapıldı ve söz konusu enfeksiyonların varlığı PCR ile araştırıldı. Pozitif bulunan örnekler moleküler karakterizasyon için dizin analizi işlemine tabi tutuldu.Bulgular: Örneklenen kedilerin toplam %45,71’i (32/70) FHV-1 ve %10’u (7/70) FCV nükleik asit varlığı yönünden pozitif olarak tespit edildi. Toplam pozitifliği bildirilen bu kedilerin,%4,29’i (3/70) her iki etken için birlikte pozitif olarak değerlendirildi. Örneklenen solunum sistemi bulgulu kedilerde FHV-1 enfeksiyonun daha yaygın olduğu görüldü; yaş bilgisi olan kedilerde enfeksiyonların her yaş grubunda oluştuğu gözlenirken; özellikle oral ve/veya orafarengeal swap örneklerinin her iki enfeksiyonun teşhisinde de duyarlı örneklerolduğu tespit edildi.Öneri: Bu enfeksiyonların, enfekte kedilerde görülmesinin yanı sıra sağlıklı görünümlü hayvanlarda da tespit edilebildiği ve söz konusu enfeksiyonların bulaşında bu durumungöz ardı edilmemesi gerekliliği ortaya çıkmaktadır. Bu nedenle klinik belirtilerin şiddetinin azaltılması, saçılımın ve persistansın en aza indirgenmesi adına özellikle toplu yaşayan, dışarıda bir arada bulunan kedilerin düzenli aşılanması üzerinde durulmalıdır. Ayrıca FHV ve FCV dışındaki etkenlerin de (M.felis, C.felis, B.bronchoseptica gibi bakterilerIer) kedilerde solunum, göz ve oral lezyonlarda bulunabileceği unutulmamalı ve bu etkenler de enfekte kedilerde kontrol edilmelidir.
Anahtar Kelime: Konular:
Molecular investigation of Feline Herpesvirus 1 (FHV-1) and Feline Calicivirus in cats with respiratory system problem
Öz: Aim: The aim of this study was to investigate the presence / prevalence of these infections in FHV (Feline Herpesvirus) and FCV (Feline Calicivirus) suspected samples, to correlate them with reported symptoms and molecular characterization of these viruses. Materials and Methods: For this purpose, a total of 119 specimens were collected from 70 from cats clinically showing respiratory system problem, 31 nasal, 30 conjunctival, 8 oral, 7 orafarengeal and 11 rectal swaps and 32 EDTA blood samples. Viral nucleic acid extraction was performed from the samples and the presence of these infections was investigated by PCR. Samples with positive results were subjected to sequence analysis for molecular characterization. Results: Sampled cats were evaluated as positive for 45.71% (32/70) FHV-1 and 10% (7/70) FCV. 4,29% (3/70) of these cats were positive for both infections. FHV-1 infection was more common in cats with respiratory system findings. While age-conscious cats were observed in all age groups; especially oral and / or orafarengeal swap samples were found to be sensitive samples in the diagnosis of both infections. Conclusion: These infections, as well as being seen in infected cats, can also be detected in healthy-looking animals and it should be noted that this should not be ignored in the transmission of these infections. Therefore, in order to reduce the severity of the clinical symptoms, to minimize the scattering and persistence, it is necessary to focus on the regular vaccination of the cats that live together, especially in the community. In addition, factors other than FHV and FCV (bacteria such as M.felis, C.felis, B.bronchoseptica) can be found in cats with respiratory, eye and oral lesions and these factors should be checked in infected cats.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | BİLGE DAĞALP S, Doğan F, FARZANI T, BABAOGLU A, KIRMIZI G, ÇABALAR M (2019). Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması. , 131 - 138. 10.15312/EurasianJVetSci.2019.236 |
| Chicago | BİLGE DAĞALP SEVAL,Doğan Fırat,FARZANI Touraj Aligholipour,BABAOGLU ALI RIZA,KIRMIZI Gülizar Acar,ÇABALAR Mehmet Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması. (2019): 131 - 138. 10.15312/EurasianJVetSci.2019.236 |
| MLA | BİLGE DAĞALP SEVAL,Doğan Fırat,FARZANI Touraj Aligholipour,BABAOGLU ALI RIZA,KIRMIZI Gülizar Acar,ÇABALAR Mehmet Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması. , 2019, ss.131 - 138. 10.15312/EurasianJVetSci.2019.236 |
| AMA | BİLGE DAĞALP S,Doğan F,FARZANI T,BABAOGLU A,KIRMIZI G,ÇABALAR M Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması. . 2019; 131 - 138. 10.15312/EurasianJVetSci.2019.236 |
| Vancouver | BİLGE DAĞALP S,Doğan F,FARZANI T,BABAOGLU A,KIRMIZI G,ÇABALAR M Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması. . 2019; 131 - 138. 10.15312/EurasianJVetSci.2019.236 |
| IEEE | BİLGE DAĞALP S,Doğan F,FARZANI T,BABAOGLU A,KIRMIZI G,ÇABALAR M "Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması." , ss.131 - 138, 2019. 10.15312/EurasianJVetSci.2019.236 |
| ISNAD | BİLGE DAĞALP, SEVAL vd. "Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması". (2019), 131-138. https://doi.org/10.15312/EurasianJVetSci.2019.236 |
| APA | BİLGE DAĞALP S, Doğan F, FARZANI T, BABAOGLU A, KIRMIZI G, ÇABALAR M (2019). Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması. Eurasian Journal of Veterinary Sciences, 35(3), 131 - 138. 10.15312/EurasianJVetSci.2019.236 |
| Chicago | BİLGE DAĞALP SEVAL,Doğan Fırat,FARZANI Touraj Aligholipour,BABAOGLU ALI RIZA,KIRMIZI Gülizar Acar,ÇABALAR Mehmet Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması. Eurasian Journal of Veterinary Sciences 35, no.3 (2019): 131 - 138. 10.15312/EurasianJVetSci.2019.236 |
| MLA | BİLGE DAĞALP SEVAL,Doğan Fırat,FARZANI Touraj Aligholipour,BABAOGLU ALI RIZA,KIRMIZI Gülizar Acar,ÇABALAR Mehmet Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması. Eurasian Journal of Veterinary Sciences, vol.35, no.3, 2019, ss.131 - 138. 10.15312/EurasianJVetSci.2019.236 |
| AMA | BİLGE DAĞALP S,Doğan F,FARZANI T,BABAOGLU A,KIRMIZI G,ÇABALAR M Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması. Eurasian Journal of Veterinary Sciences. 2019; 35(3): 131 - 138. 10.15312/EurasianJVetSci.2019.236 |
| Vancouver | BİLGE DAĞALP S,Doğan F,FARZANI T,BABAOGLU A,KIRMIZI G,ÇABALAR M Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması. Eurasian Journal of Veterinary Sciences. 2019; 35(3): 131 - 138. 10.15312/EurasianJVetSci.2019.236 |
| IEEE | BİLGE DAĞALP S,Doğan F,FARZANI T,BABAOGLU A,KIRMIZI G,ÇABALAR M "Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması." Eurasian Journal of Veterinary Sciences, 35, ss.131 - 138, 2019. 10.15312/EurasianJVetSci.2019.236 |
| ISNAD | BİLGE DAĞALP, SEVAL vd. "Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması". Eurasian Journal of Veterinary Sciences 35/3 (2019), 131-138. https://doi.org/10.15312/EurasianJVetSci.2019.236 |
