Aim: The primary aim of this study is to determine the effect of danofloxacin administrations on 8-hydroxy-2-deoxyguanosine (8- OHDG) level, oxidative stress biomarker, in sheep. In addition, to determine on heart [Troponin I, creatine kinase-MB (CK-MB) isoenzyme, lactate dehydrogenase (LDH), aspartate aminotransferase (AST)], liver [Alkaline phosphatase (ALP), alanine aminotransferase (ALT)] and kidney [Blood urea nitrogen (BUN), creatinine] damage biomarkers and hemogram parameters [White blood cell count (WBC), red blood cell count (RBC), platelet count, hemoglobin, hematocrit]. Materials and Methods: In this study, 6 mg/kg/day (Subcutaneous) dose of danofloxacin was applied to 10 sheep for 14 days. Blood samples were taken on day 0 (control), 1, 3, 5, 7, 9, 11, 13 and 15 days. Serum 8-OHDG, troponin I and CK-MB isoenzyme levels were measured with ELISA reader, while serum LDH, AST, ALT, ALP, creatinine and BUN values were determined with autoanalyzer. Hemogram parameters were determined with hemocell counter. Results: In this study, 8-OHDG levels did not change (P>0,05) statistically, while temporal elevations in troponin I, CK-MB isoenzyme and AST levels were determined (P<0,05). Statistical fluctuations (P<0,05) were determined in BUN levels, while decreases in WBC, RBC, hemoglobin and hematocrit values (P<0,05) and temporary increase in platelet level were determined (P<0,05). Conclusion: It can be stated that danofloxacin administration to sheep for 14 days does not cause systemic oxidative stress and does not cause seriously effects to the function of heart, liver, kidney and bone marrow.