This study was carried out to determine the thoughts about sexual health education of nursing students who took and did not take sexual health course in a university of Aegean region. The study was carried out with 392 students, 187 of this took sexual health course and 205 of them did not take in 2014-2015 and 2015-2016 academic year. The data of the research were collected by the researchers with a questionnaire is developed in accordance with the literature. The data of study were analyzed with using SPSS 20.0 statistical software, percentage distribution, and chi-square test. The mean age of the students was 20.8±1.3, majority of them were single (97.2%) and 69.4% of them were female. Overall, 57.9% of students had information about sexuality and 42.7% of them got information from their friends, 53.7% from media/internet. Of the students, 20.4% have had their pre-marital sexual experience. Of the students who took sexual health course 60.4% and 45.4% of students who did not take found that the level of knowledge on sexual issues was sufficient. The students 69.1% stated that sexual health course should be a compulsory course. In conclusion, it was found that students who received sexual health course think positive about sexual health education. It is very important to expand the sexual health education in universities for young people to have a healthy sexual life.