Objective: Although many new interventions including changes in diagnostic policiesare performed to increase the rates of diagnosis of brain death (BD) and organ donation in recentyears in Turkey, data about the longitudinal effects are limited. The aim of this study was tounderstand the current status of organ donation in İzmir and to find out whether new regulationshave any effect on the diagnosis of BD and organ donation rates.Materials and Methods: All patients diagnosed with BD between January 2001 and December2016 in a tertiary university hospital were included, and patient data and organ donation statuswere collected from medical records.Results: There were 303 patients diagnosed with BD during the study period. The most commonreason in the pediatric group (n=42) was traumatic brain injury (42.9%) and 12 of these patients(28.6%) became organ donors. In the adult patient group (n=261), the most common reason wasintracranial hemorrhage (39.8%) and 97 patients (37.2%) became organ donors. The rate of BDdiagnosis increased over the years (from 0.59% to 0.67% after legal regulation), but there wasno increase in organ donation rate (39.5 vs 26.5%). The most common cause of ineligibility fordonation was refusal by patient’s relatives in both pediatric and adult patients (83.3% and 86.6%respectively).Conclusion: This study shows that although the diagnosis of BD has increased over the years,organ donation rates are still low in İzmir. New strategies aiming to increase awareness and changethe perception of organ donation should be planned as soon as possible.