TY  - JOUR
TI  - Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Patients Admitted to the Intensive Care Unit of Hatay State Hospital
AB  - Objective: In this study, it was aimed to determine vancomycin resistancemechanisms, virulence genes and clonal relationships of 23 vancomycin-resistant Enterococcusfaecium (VREfae) strains isolated from patients admitted to intensive care unit of Hatay StateHospital.Materials and Methods: Minimal inhibitor concentration (MIC) values of vancomycin weredetermined by E-test and antimicrobial susceptibility of the strains was determined by discdiffusion method. The clonal relationship among VREfae isolates was determined by pulsed-fieldgel electrophoresis (PFGE). Additionally, vancomycin resistance (vanA, vanB, vanC, vanD, vanE andvanG) and virulence genes (asa1, esp, gelE, hyl and cylA) were investigated by polymerase chainreaction (PCR) method.Results: All isolates harbored vanA gene and 17 isolates (73.9%) were positive for at least onevirulence gene. The most common virulence gene was esp, which was detected in nine isolatesalone and in two isolates together with hly gene. The hly gene was detected in five isolates aloneand the other virulence genes (asa1, gelE and cylA) were not observed in any isolates tested. Allisolates showed multidrug resistance phenotype. Vancomycin MIC values of the isolates werefound to be ≥256 µg/mL by E-test. PFGE analysis revealed 14 different pulsotypes in five clusters(A, B, C, D and E). The presence of identical PFGE patterns indicated that there was a crosstransmission at hospital settings.Conclusion: Continuous surveillance of resistance patterns and molecular characteristics of VREare needed to reduce the prevalence of infections caused by VRE and to take effective controlmeasures in hospitals.
AU  - ASLANTAŞ, Özkan
AU  - BAĞCI, Fatma
AU  - SAKİN, Fatih
DO  - 10.4274/tybd.galenos.2019.20438
PY  - 2019
JO  - Türk Yoğun Bakım Dergisi
VL  - 17
IS  - 4
SN  - 2602-2974
SP  - 204
EP  - 208
DB  - TRDizin
UR  - http://search/yayin/detay/333185
ER  -