Sequential Green Extraction of Caffeine and Catechins from Green Tea

SERDAR, GÖNÜL; SÖKMEN, Münevver; Demir Özer, Ezgi

doi:10.21448/ijsm.629151

Sequential Green Extraction of Caffeine and Catechins from Green Tea

Gönül SERDAR, (Karadeniz Teknik Üniversitesi)

EZGİ DEMİR ÖZER, (Konya Gıda ve Tarım Üniversitesi)

Münevver SÖKMEN (Konya Gıda ve Tarım Üniversitesi)

International Journal of Secondary Metabolite

15 2

Yıl: 2019 Cilt: 6 Sayı: 3 Sayfa Aralığı: 283 - 291 Metin Dili: İngilizce DOI: 10.21448/ijsm.629151 İndeks Tarihi: 27-04-2020

Sequential Green Extraction of Caffeine and Catechins from Green Tea

Öz:

Separation of caffeine and catechins from tea extracts usuallyrequires conventional liquid-liquid extraction employing chloroform. Thiswork was planned to improve a green extraction technique to distunguish theseimportant chemicals from green tea. Extraction of caffeine and catechins fromTurkish green tea firstly employing MAE, and then to separate thesecompounds from each other using a SFE method were purpose of this study.Microwave assisted extraction was applied to extract tea components fromgreen tea then i) conventional liquid-liquid extraction or ii) supercritical carbondioxide fluid extraction (SFE) method was charged with the effective separationof caffeine and catechins. Initially, an ethanol: water mixture was used in a closemicrowave system under the particuler extraction situations of green teasamples (fresh, frozen or dried) picked up in three collection periods (first,second and third collection periods). MAE of tea samples was exerted under acontrolled 600 W microwave power for 4 min irradiation time at 80 Ctemperature. Then MAE crude aqueous extract was divided in to two portions.The first portion was fractionated first with chloroform to distunguish caffeinethen ethyl acetate for catechins. Caffeine and catechins were successfullyseparated. Second portion was freeze-dried and obtained lyophilized solid wasused for SFE. Caffeine (3.68% extract yield) was successfully separated fromcatechins with SFE at 250 bar and 60°C for 180 min. Constituents of the extractswere determined (caffeine and four catechins namely EGC, EC, C, EGCG) byHPLC to evaluate the effectiveness of the separation.

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SERDAR G, Demir Özer E, SÖKMEN M (2019). Sequential Green Extraction of Caffeine and Catechins from Green Tea. , 283 - 291. 10.21448/ijsm.629151
Chicago	SERDAR GÖNÜL,Demir Özer Ezgi,SÖKMEN Münevver Sequential Green Extraction of Caffeine and Catechins from Green Tea. (2019): 283 - 291. 10.21448/ijsm.629151
MLA	SERDAR GÖNÜL,Demir Özer Ezgi,SÖKMEN Münevver Sequential Green Extraction of Caffeine and Catechins from Green Tea. , 2019, ss.283 - 291. 10.21448/ijsm.629151
AMA	SERDAR G,Demir Özer E,SÖKMEN M Sequential Green Extraction of Caffeine and Catechins from Green Tea. . 2019; 283 - 291. 10.21448/ijsm.629151
Vancouver	SERDAR G,Demir Özer E,SÖKMEN M Sequential Green Extraction of Caffeine and Catechins from Green Tea. . 2019; 283 - 291. 10.21448/ijsm.629151
IEEE	SERDAR G,Demir Özer E,SÖKMEN M "Sequential Green Extraction of Caffeine and Catechins from Green Tea." , ss.283 - 291, 2019. 10.21448/ijsm.629151
ISNAD	SERDAR, GÖNÜL vd. "Sequential Green Extraction of Caffeine and Catechins from Green Tea". (2019), 283-291. https://doi.org/10.21448/ijsm.629151

APA	SERDAR G, Demir Özer E, SÖKMEN M (2019). Sequential Green Extraction of Caffeine and Catechins from Green Tea. International Journal of Secondary Metabolite, 6(3), 283 - 291. 10.21448/ijsm.629151
Chicago	SERDAR GÖNÜL,Demir Özer Ezgi,SÖKMEN Münevver Sequential Green Extraction of Caffeine and Catechins from Green Tea. International Journal of Secondary Metabolite 6, no.3 (2019): 283 - 291. 10.21448/ijsm.629151
MLA	SERDAR GÖNÜL,Demir Özer Ezgi,SÖKMEN Münevver Sequential Green Extraction of Caffeine and Catechins from Green Tea. International Journal of Secondary Metabolite, vol.6, no.3, 2019, ss.283 - 291. 10.21448/ijsm.629151
AMA	SERDAR G,Demir Özer E,SÖKMEN M Sequential Green Extraction of Caffeine and Catechins from Green Tea. International Journal of Secondary Metabolite. 2019; 6(3): 283 - 291. 10.21448/ijsm.629151
Vancouver	SERDAR G,Demir Özer E,SÖKMEN M Sequential Green Extraction of Caffeine and Catechins from Green Tea. International Journal of Secondary Metabolite. 2019; 6(3): 283 - 291. 10.21448/ijsm.629151
IEEE	SERDAR G,Demir Özer E,SÖKMEN M "Sequential Green Extraction of Caffeine and Catechins from Green Tea." International Journal of Secondary Metabolite, 6, ss.283 - 291, 2019. 10.21448/ijsm.629151
ISNAD	SERDAR, GÖNÜL vd. "Sequential Green Extraction of Caffeine and Catechins from Green Tea". International Journal of Secondary Metabolite 6/3 (2019), 283-291. https://doi.org/10.21448/ijsm.629151