Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas

SENER, SILA OZLEM; ÖZGEN, Ufuk; AKKAYA, Şeyda; badem, merve; ALİYAZICIOĞLU, Rezzan; Abudayyak, Mahmoud; Korkmaz, Nuriye; Öztaş, Ezgi

Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas

Nuriye KORKMAZ, (Karadeniz Teknik Üniversitesi)

Sıla Özlem ŞENER, (Karadeniz Teknik Üniversitesi)

Şeyda AKKAYA, (Karadeniz Teknik Üniversitesi)

Merve BADEM, (Karadeniz Teknik Üniversitesi)

Rezzan ALİYAZICIOĞLU, (Karadeniz Teknik Üniversitesi)

Mahmoud ABUDAYYAK, (Karadeniz Teknik Üniversitesi)

Ezgi ÖZTAŞ, (İstanbul Üniversitesi)

Ufuk ÖZGEN (Karadeniz Teknik Üniversitesi)

Türk Biyokimya Dergisi

10 5

Yıl: 2019 Cilt: 44 Sayı: 3 Sayfa Aralığı: 278 - 288 Metin Dili: İngilizce İndeks Tarihi: 05-05-2020

Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas

Öz:

tyrosinase inhibitory (TI) activities, and phenolic profileswith a new high performance liquid chromatography(HPLC) method of green, white and black teas.Methods: Antioxidant activities of the teas were examinedby means of scavenging of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidantpower (FRAP), and cupric reducing antioxidant capacity(CUPRAC). The phenolic contents were investigated bymeans of HPLC. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay was used to evaluatethe cytotoxic potential of tea extracts in cancer and normalcell lines. TI activity was investigated against diphenolase(DOPA), using kojic acid as a positive control.Results: The green tea extract exhibited stronger antioxidantthan white and black tea extracts. The greentea contained syringaldehyde, p-coumaric acid, benzoicacid, and quercetin. The white tea extract had thehighest tyrosinase inhibitor activity. The extracts exhibitedhigher cytotoxic potential toward cancer cells thannormal cells. The methanol extract of green tea had thehighest cytotoxic potential, while the water extracts wereless toxic.Conclusion: The green, white, and black teas can beregarded as an important in terms of rich antioxidant,tyrosinase inhibitor, and cytotoxic activities in both thepharmaceutical and food industries.

Anahtar Kelime:

Konular: Diş Hekimliği Toksikoloji Biyokimya ve Moleküler Biyoloji Farmakoloji ve Eczacılık

Yeşil, beyaz ve siyah çayın antioksidan, sitotoksik, tirozinaz inhibitör aktiviteleri ve fenolik profillerinin araştırılması

Öz:

Amaç: Yeşil, beyaz ve siyah çayın antioksidan, sitotoksik ve tirozinaz inhibitör (TI) aktivitelerini ve yeni yüksek performanslı sıvı kromatografisi (HPLC) metodu ile fenolik içeriklerini belirlemek. Metod: Çayların antioksidan aktiviteleri; 2,2-difenil-1-pikril hidrazil (DPPH) serbest radikalin süpürülmesi, demir indirgeyici antioksidan güç (FRAP) ve bakır indirgeyici antioksidan kapasite (CUPRAC) ile incelendi. Fenolik içeriği HPLC ile incelendi. MTT yöntemi normal ve kanser hücre hatlarında çay ekstrelerinin sitotoksik potansiyelini değerlendirmek için kullanıldı. TI aktivite, pozitif kontrol olarak kojik asit kullanılarak difenolaza (DOPA) karşı incelendi.Bulgular: Yeşil çay ekstresi beyaz ve siyah çay ekstresinden daha güçlü antioksidan aktivite gösterdi. Yeşil çay şiringaldehid, p-kumarik asit, benzoik asit ve kersetin içerdi. Beyaz çay ekstresi en yüksek tirozinaz inhibitör aktivite gösterdi. Ekstreler, kanser hücrelerine karşı normal hücrelerden daha yüksek sitotoksik güce sahipti. Yeşil çayın su ekstresi daha az toksik iken, metanol ekstresi en yüksek sitotoksik güce sahipti. Sonuç: Yeşil, beyaz ve siyah çay hem farmasötik hem de besin endüstrisinde zengin antioksidan, tirozinaz inhibitör ve sitotoksik aktiviteleri açısından önemli olarak düşünülebilir.

Anahtar Kelime:

Konular: Diş Hekimliği Toksikoloji Biyokimya ve Moleküler Biyoloji Farmakoloji ve Eczacılık

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Fridovich I. The biology of oxygen radicals. Science 1978;201:875–80.
2. Harman D. Aging: a theory based on free radical and radiation chemistry. J Gerontol 1956;11:298–300.
3. Duthie GG. Lipid peroxidation. Eur J Clin Nutr 1993;47:759–64.
4. Weisburger JH. Mechanism of action of antioxidants as exemplified in vegetables, tomatoes and tea. Food Chem Toxicol 1999;37:943–8.
5. Balentine DA, Wiseman SA, Bouwens LC. The chemistry of tea flavonoids. Crit Rev Food Sci 1997;37:693–704.
6. Salah N, Miller NJ, Paganga G, Tijburg L, Bolwell GP, Rice-Evans C. Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants. Arch Biochem Biophys 1995;322:339–46.
7. Jovanovic S, Steenken S, Tosic M, Marjanovic B, Simic MG. Flavonoids as antioxidants. J Am Chem Soc 1994;116:4846–51.
8. Hilal Y, Engelhardt U. Characterisation of white tea comparison to green and black tea. J Ver Leb 2007;2:414–21.
9. Vaibhav S, Lakshman K. Tyrosinase enzyme inhibitory activity of selected Indian herbs. Int J Pharm Biomed Res 2012;3:977–82.
10. Kim YJ, Uyama H. Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell Mol Life Sci 2005;62:1707–23.
11. Parvez S, Kang M, Chung HS, Bae H. Naturally occurring tyrosinase inhibitors: mechanism and applications in skin health, cosmetics and agriculture industries. Phytother Res 2007;21:805–16.
12. Singh J. International Conference on Harmonization (ICH) of Technical Requirements for the Registration of Pharmaceuticals for Human Use Validation of Analytical Procedures: Methodology, ICH-Q2B, Geneva, 1996.
13. Singleton VL, Rossi JA Jr. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 1965;16:144–58.
14. Kolayli S, Sahin H, Aliyazicioglu R, Sesli E. Phenolic components and antioxidant activity of three edible wild mushrooms from Trabzon, Turkey. Chem Nat Comp 2012;48:137–40.
15. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 1996;239:70–6.
16. Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songk J Sci Technol 2004;26:211–9.
17. Apak R, Güçlü K, Ozyürek M, Karademir SE, Erça E. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. Int J Food Sci Nutr 2006;57:292–304.
18. Abudayyak M, Özdemir Nath E, Özhan G. Toxic potentials of ten herbs commonly used for aphrodisiac effect in Turkey. Turk J Med Sci 2015;45:496–506.
19. Masuda T, Yamashita D, Takeda Y, Yonemori S. Screening for tyrosinase inhibitors among extracts of seashore plants and identification of potent inhibitors from Garcinia subelliptica. Biosci Biotechnol Biochem 2005;69:197–201.
20. Rosenthal I, Wolfram E, Peter S, Meier B. Validated method for the analysis of frangulins A and B and glucofrangulins A and B using HPLC and UHPLC. J Nat Prod 2014;28:489–96.
21. Stanikunaite R, Khan SI, Trappe JM, Ross SA. Cyclooxygenase-2 ınhibitory and antioxidant compounds from the truffle Elaphomyces granulatus. Phytother Res 2009;23:575–8.
22. Amir M, Agarwal HK. Role of COX-2 selective inhibitors for prevention and treatment of cancer. Pharmazie 2005;60:563–70.
23. Naga Vamsi Krishna A, Nadeem MD, Pardha Saradhi M, Mahendran B, Bharathi S. Cumulative activity of the p-coumaric acid and syringaldehyde for antimicrobial activity of different microbial strains. Eur J Exp Bio 2014;4:40–3.
24. Khetan SK. Endocrine disruptors in the environment. New Jersey: John Wiley & Sons, 2014:109–15.
25. Pugazhendhi D, Pope GS, Darbre PD. Oestrogenic activity of p-hydroxybenzoic acid (common metabolite of paraben esters) and methylparaben in human breast cancer cell lines. J Appl Toxicol 2005;25:301–9.
26. Fukushima Y, Ohie T, Yonekawa Y, Yonemoto K, Aizawa H, Mori Y, et al. Coffee and green tea as a large source of antioxidant polyphenols in the japanese population. J Agr Food Chem 2009;57:1253–9.
27. Hertog MG, Hollman PC, Katan MB. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J Agr Food Chem 1992;40:2379–83.
28. Benzie IF, Szeto YT. Total antioxidant capacity of teas by the ferric reducing/antioxidant power assay. J Agr Food Chem 1999;47:633–66.
29. Natella F, Nardini M, Giannetti I, Dattilo C, Scaccini C. Coffee drinking influences plasma antioxidant capacity in humans. J Agr Food Chem 2002;50:6211–6.
30. Lin YL, Juan IM, Chen YL, Liang YC, Lin JK. Composition of polyphenols in fresh tea leaves and associations of their oxygenradical- absorbing capacity with antiproliferative actions in fibroblast cells. J Agr Food Chem 1996;44:1387–94.
31. Aliyazicioglu R, Yildiz O, Sahin H, Eyupoglu OE, Ozkan MT, Karaoğlu ŞA, et al. Phenolic components and antioxidant activity of Prunus spinosa from Gumushane, Turkey. Chem Nat Comp 2015;51:346–9.
32. Anissi J, El Hassouni M, Ouardaoui A, Sendide K. A comparative study of the antioxidant scavenging activity of green tea, black tea and coffee extracts: a kinetic approach. Food Chem 2014;150:438–47.
33. Jungmin O, Heonjoo J, Ah Reum C, Sung-Jin K, Jaejoon H. Antioxidant and antimicrobial activities of various leafy herbal teas. Food Control 2013;31:403–9.
34. Bode AM, Dong Z. Epigallocatechin 3-gallate and green tea catechins: united they work, divided they fail. Cancer Prev Res 2009;2:514–7.
35. Brown MD. Green tea (Camellia sinensis) extract and its possible role in the prevention of cancer. Altern Med Rev 1999;4:360–70.
36. Das D, Mukherjee S, Das AS, Mukherjee M, Mitra C. Aqueous extract of black tea (Camellia sinensis) prevents ethanol + cholecystokinin-induced pancreatitis in a rat model. Life Sci 2006;78:2194–203.
37. Galati G, Lin A, Sultan AM, O’Brien PJ. Cellular and in vivo hepatotoxicity caused by green tea phenolic acids and catechins. Free Radic Biol Med 2006;40:570–80.
38. Lambert JD, Kennett MJ, Sang S, Reuhl KR, Ju J, Yang CS, et al. Hepatotoxicity of high oral dose (-)-epigallocatechin-3-gallate in mice. Food Chem Toxicol 2010;48:409–16.
39. No JK, Soung DT, Kim YJ, Shim KH, Jun YS, Rhee SH, et al. Inhibition of tyrosinase by green tea components. Life Sci 1999;65:241–6.
40. Jo YH, Yuk HG, Lee JH, Kim JC, Kim R, Lee S-C. Antioxidant, tyrosinase inhibitory, and acetylcholinesterase inhibitory activities of green tea (Camellia sinensis L.) seed and its pericarp. Food Sci Biotechnol 2012;21:761–8.

APA	Korkmaz N, SENER S, AKKAYA Ş, badem m, ALİYAZICIOĞLU R, Abudayyak M, Öztaş E, ÖZGEN U (2019). Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas. , 278 - 288.
Chicago	Korkmaz Nuriye,SENER SILA OZLEM,AKKAYA Şeyda,badem merve,ALİYAZICIOĞLU Rezzan,Abudayyak Mahmoud,Öztaş Ezgi,ÖZGEN Ufuk Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas. (2019): 278 - 288.
MLA	Korkmaz Nuriye,SENER SILA OZLEM,AKKAYA Şeyda,badem merve,ALİYAZICIOĞLU Rezzan,Abudayyak Mahmoud,Öztaş Ezgi,ÖZGEN Ufuk Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas. , 2019, ss.278 - 288.
AMA	Korkmaz N,SENER S,AKKAYA Ş,badem m,ALİYAZICIOĞLU R,Abudayyak M,Öztaş E,ÖZGEN U Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas. . 2019; 278 - 288.
Vancouver	Korkmaz N,SENER S,AKKAYA Ş,badem m,ALİYAZICIOĞLU R,Abudayyak M,Öztaş E,ÖZGEN U Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas. . 2019; 278 - 288.
IEEE	Korkmaz N,SENER S,AKKAYA Ş,badem m,ALİYAZICIOĞLU R,Abudayyak M,Öztaş E,ÖZGEN U "Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas." , ss.278 - 288, 2019.
ISNAD	Korkmaz, Nuriye vd. "Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas". (2019), 278-288.

APA	Korkmaz N, SENER S, AKKAYA Ş, badem m, ALİYAZICIOĞLU R, Abudayyak M, Öztaş E, ÖZGEN U (2019). Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas. Türk Biyokimya Dergisi, 44(3), 278 - 288.
Chicago	Korkmaz Nuriye,SENER SILA OZLEM,AKKAYA Şeyda,badem merve,ALİYAZICIOĞLU Rezzan,Abudayyak Mahmoud,Öztaş Ezgi,ÖZGEN Ufuk Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas. Türk Biyokimya Dergisi 44, no.3 (2019): 278 - 288.
MLA	Korkmaz Nuriye,SENER SILA OZLEM,AKKAYA Şeyda,badem merve,ALİYAZICIOĞLU Rezzan,Abudayyak Mahmoud,Öztaş Ezgi,ÖZGEN Ufuk Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas. Türk Biyokimya Dergisi, vol.44, no.3, 2019, ss.278 - 288.
AMA	Korkmaz N,SENER S,AKKAYA Ş,badem m,ALİYAZICIOĞLU R,Abudayyak M,Öztaş E,ÖZGEN U Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas. Türk Biyokimya Dergisi. 2019; 44(3): 278 - 288.
Vancouver	Korkmaz N,SENER S,AKKAYA Ş,badem m,ALİYAZICIOĞLU R,Abudayyak M,Öztaş E,ÖZGEN U Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas. Türk Biyokimya Dergisi. 2019; 44(3): 278 - 288.
IEEE	Korkmaz N,SENER S,AKKAYA Ş,badem m,ALİYAZICIOĞLU R,Abudayyak M,Öztaş E,ÖZGEN U "Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas." Türk Biyokimya Dergisi, 44, ss.278 - 288, 2019.
ISNAD	Korkmaz, Nuriye vd. "Investigation of antioxidant, cytotoxic, tyrosinase inhibitory activities, and phenolic profiles of green, white, and black teas". Türk Biyokimya Dergisi 44/3 (2019), 278-288.