TY  - JOUR
TI  - Improvement of the in vitro regeneration and Agrobacterium-mediated genetic transformation of Medicago sativa L.
AB  - : Alfalfa is a fodder crop that accounts for one of the best sources of protein and is widely cultivated around the world. In vitroregeneration of alfalfa has been studied earlier; however, most of the studies were almost intervened with callus formation. In this study,3 explant sources (cotyledonary node, hypocotyl, and root crown) of two Turkish cultivars (Nimet and Savaş) were excised from youngseedlings. Explants were subjected to different concentrations of BAP, BAP–IBA, and TDZ to evaluate the direct in vitro regenerationpotential of selected plant parts. Moreover, we transformed the alfalfa plant with pBin19 harboring 35s.GUS-INT_35s.nptII constructto investigate the transformation efficiency and regeneration frequency after bacterial inoculation. The transformation was carried outby Agrobacterium tumefaciens strain GV2260. The highest mean number of regenerated shoots per explant was recorded as 8.5, 6.66,and 6.33 shoots per explant after cotyledonary node explants were treated with BAP (0.40 mg/L), BAP–IBA (1.25–0.06 mg/L), andTDZ (0.55 mg/L), respectively. The highest gene transformation frequency was 9.52% and 6.19% based on PCR and GUS assays. Theregeneration frequency was decreased by up to 48.1% under kanamycin selection pressure. The effect of cultivar on shoot regenerationfrequency, mean number of regenerated shoots, and gene transformation efficiency was significant. This study contributes to in vitroregeneration of alfalfa crop and its genetic transformation which could be utilized in future gene transformation studies.
AU  - KHABBAZI, Saber Delpasand
AU  - NOFOUZI, Farzad
AU  - OĞUZ, Muhammet Çağrı
AU  - ergul, ali
DO  - 10.3906/tar-1804-52
PY  - 2019
JO  - Turkish Journal of Agriculture and Forestry
VL  - 43
IS  - 1
SN  - 1300-011X
SP  - 96
EP  - 104
DB  - TRDizin
UR  - http://search/yayin/detay/335543
ER  -