Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer

Cansu KÜÇÜKKÖSE, (İzmir Yüksek Teknoloji Enstitüsü)
Özden Yalçın ÖZUYSAL (İzmir Yüksek Teknoloji Enstitüsü)
Turkish Journal of Biology
1 0
Yıl: 2019 Cilt: 43 Sayı: 1 Sayfa Aralığı: 43 - 76 Metin Dili: İngilizce DOI: 10.3906/biy-1808-58 İndeks Tarihi: 10-05-2020

Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer

Öz:
Metastasis is the main reason for death in breast cancer. Understanding the molecular players in metastasis is crucial fordiagnostic and therapeutic purposes. Notch signalling plays an oncogenic role in breast tumorigenesis and is involved in metastasis.Downstream mediators of Notch signalling in prometastatic processes are not yet fully discovered. Here we aimed to investigate whetherNotch signalling regulates the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20, which are involved in prometastaticprocesses, in breast cell lines. To this end, expression of the selected genes was analysed following Notch activation by overexpression ofthe Notch1 intracellular domain in the normal breast epithelial cell line MCF10A, and inhibition by silencing of the Notch transcriptionalmediator RBPjκ in the breast cancer cell line MDA MB 231. SEMA3C and HMGA2 mRNA were decreased, while CXCL14 and CXCR7mRNA were increased significantly in response to Notch activation in MCF10A cells. Notch inhibition in MDA MB 231 cells significantlydecreased HMGA2 and CCL20 mRNA. Protein levels were not significantly altered by Notch modulation. In conclusion, we showed thatNotch signalling regulates expression of SEMA3C, CXCL14, CCL20, CXCR7, and HMGA2, which are prominent candidate genes thatmight function downstream of Notch to induce prometastatic processes.
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA KÜÇÜKKÖSE C, YALÇIN ÖZUYSAL Ö (2019). Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. , 43 - 76. 10.3906/biy-1808-58
Chicago KÜÇÜKKÖSE Cansu,YALÇIN ÖZUYSAL ÖZDEN Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. (2019): 43 - 76. 10.3906/biy-1808-58
MLA KÜÇÜKKÖSE Cansu,YALÇIN ÖZUYSAL ÖZDEN Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. , 2019, ss.43 - 76. 10.3906/biy-1808-58
AMA KÜÇÜKKÖSE C,YALÇIN ÖZUYSAL Ö Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. . 2019; 43 - 76. 10.3906/biy-1808-58
Vancouver KÜÇÜKKÖSE C,YALÇIN ÖZUYSAL Ö Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. . 2019; 43 - 76. 10.3906/biy-1808-58
IEEE KÜÇÜKKÖSE C,YALÇIN ÖZUYSAL Ö "Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer." , ss.43 - 76, 2019. 10.3906/biy-1808-58
ISNAD KÜÇÜKKÖSE, Cansu - YALÇIN ÖZUYSAL, ÖZDEN. "Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer". (2019), 43-76. https://doi.org/10.3906/biy-1808-58
APA KÜÇÜKKÖSE C, YALÇIN ÖZUYSAL Ö (2019). Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. Turkish Journal of Biology, 43(1), 43 - 76. 10.3906/biy-1808-58
Chicago KÜÇÜKKÖSE Cansu,YALÇIN ÖZUYSAL ÖZDEN Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. Turkish Journal of Biology 43, no.1 (2019): 43 - 76. 10.3906/biy-1808-58
MLA KÜÇÜKKÖSE Cansu,YALÇIN ÖZUYSAL ÖZDEN Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. Turkish Journal of Biology, vol.43, no.1, 2019, ss.43 - 76. 10.3906/biy-1808-58
AMA KÜÇÜKKÖSE C,YALÇIN ÖZUYSAL Ö Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. Turkish Journal of Biology. 2019; 43(1): 43 - 76. 10.3906/biy-1808-58
Vancouver KÜÇÜKKÖSE C,YALÇIN ÖZUYSAL Ö Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. Turkish Journal of Biology. 2019; 43(1): 43 - 76. 10.3906/biy-1808-58
IEEE KÜÇÜKKÖSE C,YALÇIN ÖZUYSAL Ö "Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer." Turkish Journal of Biology, 43, ss.43 - 76, 2019. 10.3906/biy-1808-58
ISNAD KÜÇÜKKÖSE, Cansu - YALÇIN ÖZUYSAL, ÖZDEN. "Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer". Turkish Journal of Biology 43/1 (2019), 43-76. https://doi.org/10.3906/biy-1808-58
Sayfa Oluşturulma Tarihi
23-04-2024 11:34

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