Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization

IMAMOGLU, RIZVAN; ATAMAN, Bahadır; DÜZGÜN, Duygu; KUDUĞ CEYLAN, HÜLYA; GÖKÇE, İSA

Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization

Hülya KUDUĞ, (Tokat Gaziosmanpaşa Üniversitesi)

Bahadır ATAMAN, (Tokat Gaziosmanpaşa Üniversitesi)

Rizvan İMAMOĞLU, (Bartın Üniversitesi)

Duygu DÜZGÜN, (Tokat Gaziosmanpaşa Üniversitesi)

İsa GÖKÇE (Tokat Gaziosmanpaşa Üniversitesi)

International Advanced Researches and Engineering Journal

32 0

Yıl: 2019 Cilt: 3 Sayı: 1 Sayfa Aralığı: 20 - 25 Metin Dili: İngilizce İndeks Tarihi: 14-05-2020

Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization

Öz:

New and improved genetic engineered variants of fluorescent proteins (FPs) have become usefultools for bioimaging in biomedical researches. Red fluorescent proteins (RFPs) first derivedfrom the sea anemone Discosoma show high performance in vivo labeling and imaging. mCherryis a member of RFPs which has very high photostability, resistant to photo bleaching and rapidmaturation. These advantages ensure that mCherry can be successfully fused to many proteinsand widely used for quantitative imaging techniques. In this study, the constructed recombinantplasmid pBADCherry was expressed in Escherichia coli BL21(AI) then culture conditions,inducer concentration and induction time were optimized. Results of sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) analysis demonstrated that 5 hours induction at0.04% of arabinose concentration was optimal for the highest mCherry yield. The expression ofhexa histidine-tagged (6xHis) recombinant mCherry was induced by arabinose and purificationperformed using nickel (Ni2+) affinity chromatography. High throughput expression of 81 mgfluorescent protein from a liter of E. coli culture carried out in bioreactor.

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APA	KUDUĞ CEYLAN H, ATAMAN B, IMAMOGLU R, DÜZGÜN D, GÖKÇE İ (2019). Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization. , 20 - 25.
Chicago	KUDUĞ CEYLAN HÜLYA,ATAMAN Bahadır,IMAMOGLU RIZVAN,DÜZGÜN Duygu,GÖKÇE İSA Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization. (2019): 20 - 25.
MLA	KUDUĞ CEYLAN HÜLYA,ATAMAN Bahadır,IMAMOGLU RIZVAN,DÜZGÜN Duygu,GÖKÇE İSA Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization. , 2019, ss.20 - 25.
AMA	KUDUĞ CEYLAN H,ATAMAN B,IMAMOGLU R,DÜZGÜN D,GÖKÇE İ Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization. . 2019; 20 - 25.
Vancouver	KUDUĞ CEYLAN H,ATAMAN B,IMAMOGLU R,DÜZGÜN D,GÖKÇE İ Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization. . 2019; 20 - 25.
IEEE	KUDUĞ CEYLAN H,ATAMAN B,IMAMOGLU R,DÜZGÜN D,GÖKÇE İ "Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization." , ss.20 - 25, 2019.
ISNAD	KUDUĞ CEYLAN, HÜLYA vd. "Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization". (2019), 20-25.

APA	KUDUĞ CEYLAN H, ATAMAN B, IMAMOGLU R, DÜZGÜN D, GÖKÇE İ (2019). Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization. International Advanced Researches and Engineering Journal, 3(1), 20 - 25.
Chicago	KUDUĞ CEYLAN HÜLYA,ATAMAN Bahadır,IMAMOGLU RIZVAN,DÜZGÜN Duygu,GÖKÇE İSA Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization. International Advanced Researches and Engineering Journal 3, no.1 (2019): 20 - 25.
MLA	KUDUĞ CEYLAN HÜLYA,ATAMAN Bahadır,IMAMOGLU RIZVAN,DÜZGÜN Duygu,GÖKÇE İSA Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization. International Advanced Researches and Engineering Journal, vol.3, no.1, 2019, ss.20 - 25.
AMA	KUDUĞ CEYLAN H,ATAMAN B,IMAMOGLU R,DÜZGÜN D,GÖKÇE İ Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization. International Advanced Researches and Engineering Journal. 2019; 3(1): 20 - 25.
Vancouver	KUDUĞ CEYLAN H,ATAMAN B,IMAMOGLU R,DÜZGÜN D,GÖKÇE İ Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization. International Advanced Researches and Engineering Journal. 2019; 3(1): 20 - 25.
IEEE	KUDUĞ CEYLAN H,ATAMAN B,IMAMOGLU R,DÜZGÜN D,GÖKÇE İ "Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization." International Advanced Researches and Engineering Journal, 3, ss.20 - 25, 2019.
ISNAD	KUDUĞ CEYLAN, HÜLYA vd. "Production of red fluorescent protein (mCherry) in an inducible E. coli expression system in a bioreactor, purification and characterization". International Advanced Researches and Engineering Journal 3/1 (2019), 20-25.