TY  - JOUR
TI  - Evaluation of VEGF, Cytokeratin-19 and Caspase 3 Immunolocalization in the Lung Tissue of Rat with Experimentally Induced Diabetes
AB  - Diabetes Mellitus (DM) manifests itself with changes in the functional structure of the lungs and impairments in gas exchange. These changesin diabetic lung tissue may be due to various factors. Our aim in this study is to correlate the damage of diabetes with lung tissue in terms of VEGF, CK19, caspase 3 immunolocalizations. In this study, animals were divided into 4 groups, 60 mg/kg streptozotocin was given to each of thegroups with experimental diabetes and the physiological saline solution was given intraperitoneally to the control group. On days 7 and 14 of theexperiment, diabetic and control groups were euthanized, and lung tissues were removed. Tissue samples were evaluated histochemically andimmunohistochemically by monitoring with standard light microscopy. In the diabetic group, the localization of CK19 and Caspase 3 increasedon the 7th and 14th days compared to the control group, but the immunolocalization of VEGF decreased. Based on our findings, it was determined that lung tissue was one of the target organs of diabetes. The increase in pulmonary parenchyma due to hyperglycemia is accepted as a source of fibrosis. We concluded that due to increased CK19 localization of fibrosis source, decreased VEGF localization has increased apoptosis in the pulmonary capillary endothelium, which has a significant role in the blood-air barrier in the lung parenchyma, especially in endothelial cells.
AU  - SARAYDIN, Serpil ÜNVER
AU  - ŞAHİN İNAN, Zeynep Deniz
DO  - 10.9775/kvfd.2018.21141
PY  - 2019
JO  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
VL  - 25
IS  - 3
SN  - 1300-6045
SP  - 415
EP  - 420
DB  - TRDizin
UR  - http://search/yayin/detay/337341
ER  -