The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis

ETEM, Ebru; BAYINDIR, Yasar; yesilada, elif; elmasdag, sirvan; karaca, zeynal mete; Kayabas, Uner; KARACA, Gamze; KAYHAN, BASAK; ALTUNIŞIK TOPLU, SİBEL

doi:10.14744/nci.2018.96992

The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis

Gamze Karaca, (İnönü Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji ve Genetik Anabilim Dalı, Malatya, Türkiye)

Zeynal KARACA, (İnönü Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji ve Genetik Anabilim Dalı, Malatya, Türkiye)

Basak Kayhan, (İnönü Üniversitesi, Tıp Fakültesi, Karaciğer Nakli Enstitüsü, Transplantasyon İmmünoloji Laboratuvarı, Malatya, Türkiye)

Yasar Bayindir, (İnönü Üniversitesi, Tıp Fakültesi, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı, Malatya, Türkiye)

ÜNER KAYABAŞ, (İnönü Üniversitesi, Tıp Fakültesi, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Anabilim Dalı, Malatya, Türkiye)

Sibel Toplu, (Malatya Devlet Hastanesi, Enfeksiyon Hastalıkları Bölümü, Malatya, Türkiye)

Sirvan Elmasdag, (Malatya Devlet Hastanesi, Enfeksiyon Hastalıkları Bölümü, Malatya, Türkiye)

Ebru Onalan, (Fırat Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Elazığ, Türkiye)

Elif Yesilada (İnönü Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji ve Genetik Anabilim Dalı, Malatya, Türkiye)

İstanbul Kuzey Klinikleri

3 0

Yıl: 2019 Cilt: 6 Sayı: 2 Sayfa Aralığı: 117 - 123 Metin Dili: İngilizce DOI: 10.14744/nci.2018.96992 İndeks Tarihi: 21-05-2020

The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis

Öz:

OBJECTIVE: Brucellosis is a zoonotic disease caused by Brucella in domestic and wild animals. It also causes systemicdiseases with the involvement of different parts of the human body. An efficient innate immune response is crucial to curebrucellosis with optimum antibiotic treatment. The inflammasomes are innate immune system receptors and sensors thatregulate the activation of cysteine-dependent aspartate specific protease-1 (caspase-1) and caspase-1-induced cell deathprocess known as pyroptosis. The aim of the present study was to investigate the expression levels of CASPASE-1 and associated inflammasomes AIM2, NLRP3, and NLRC4 to analyze their relationship with the inflammatory cytokine interleukin (IL)-1β, IL-18, and interferon-gamma (IFN-γ) in peripheral blood samples of patients with acute brucellosis with healthy controls.METHODS: Peripheral blood samples were obtained from 20 healthy volunteers and 20 patients with acute brucellosis. RNAand serum samples were isolated to examine the expression levels of AIM2, NLRP3, NLRC4, and CASPASE-1 by real-timepolymerase chain reaction, and IL-1β, IL-18, and IFN-γ were measured by enzyme-linked immunosorbent assay.RESULTS: In the acute brucellosis group, AIM2 and NLRC4 expressions were significantly higher than in healthy volunteers.A significant increase on caspase-1 expression in patients with acute brucellosis was not observed. Serum IL-18 and IFN-γlevels were significantly higher in patients with acute brucellosis than in healthy controls.CONCLUSION: Caspase-1-related inflammasomes are sufficiently activated to induce the secretion of cytokines, such asIFN-γ and IL-18, to induce cellular immune response. Caspase-1 activation level should be investigated at different periods ofdisease in a group with high number of patients to understand the role of pyroptosis and caspase-1 in brucellosis.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Doganay M, Aygen B. Human Brucellosis: An overview. Int J Infect Dis. 2003;7:173–82. [CrossRef ]
2. Pappas G, Akritidis N, Bosilkovski M, Tsianos E. Brucellosis. N Engl J Med 2005;352:2325–36. [CrossRef ]
3. Xavier MN, Paixoa TA, den Hartigh AB, Tsolis RM, Santos RL. Pathogenesis of Brucella spp. Open Vet Sci J 2010; 4:109–18. [CrossRef ]
4. Skendros P, Pappas G, Boura P. Cell-mediated immunity in human brucellosis. Microbes Infect 2011;13:134–42. [CrossRef ]
5. Skendros P, Boura P. Immunity to brucellosis. Rev Sci Tech 2013;32:137–47. [CrossRef ]
6. Baldwin CL, Goenka R. Host immune responses to the intracellular bacteria Brucella: does the bacteria instruct the host to facilitate chronic infection? Crit Rev Immunol 2006;26:407–42. [CrossRef ]
7. Kayhan B, Kayabas Ü, Kolgelier S, Otlu B, Gül M, Kurtoglu EL, et al. Mystery of Immune Response in Relapsed Brucellosis: Immunophenotyping and Multiple Cytokine Analysis. 2016; 5:1–9. [CrossRef ]
8. Integrated Dynamics of Innate and Adaptive Immunity. In: Murphy K, Weaver C, editors. Janeways Immunobiology. New York: Garland Science; 2017. p. 445–91.
9. Guo H, Callaway JB, Ting JP. Inflammasomes: mechanism of action, role in disease, and therapeutics. Nat Med 2015;21:677–87. [CrossRef ]
10. Martinon F, Burns K, Tschopp J. The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta. Mol Cell 2002;10:417–26. [CrossRef ]
11. Monie TP. The Canonical Inflammasome: A Macromolecular Complex Driving Inflammation. Subcell Biochem 2017;83:43–73. [CrossRef ]
12. Strowig T, Henao-Mejia J, Elinav E, Flavell R. Inflammasomes in health and disease. Nature 2012;481:278–86. [CrossRef ]
13. Ariza J, Corredoira J, Pallares R, Viladrich PF, Rufi G, Pujol M, et al. Characteristics of and risk factors for relapse of brucellosis in humans. Clin Infect Dis 1995;20:1241–9. [CrossRef ]
14. Franco MP, Mulder M, Smits HL. Persistence and relapse in brucellosis and need for improved treatment. Trans R Soc Trop Med Hyg 2007;101:854–5. [CrossRef ]
15. Ögredici Ö, Erb S, Langer I, Pilo P, Kerner A, Haack HG, et al. Brucellosis reactivation after 28 years. Emerg Infect Dis 2010;16:2021–2.
16. Gomes MT, Campos PC, Oliveira FS, Corsetti PP, Bortoluci KR, Cunha LD, et al. Critical role of ASC inflammasomes and bacterial type IV secretion system in caspase-1 activation and host innate resistance to Brucella abortus infection. J Immunol 2013;190:3629–38. [CrossRef ]
17. Rathinam VA, Jiang Z, Waggoner SN, Sharma S, Cole LE, Waggoner L, et al. The AIM2 inflammasome is essential for host defense against cytosolic bacteria and DNA viruses. Nat Immunol 2010;11:395–402.
18. De Nardo D, Latz E. NLRP3 inflammasomes link inflammation and metabolic disease. Trends Immunol 2011;32:373–9. [CrossRef ]
19. Wen H, Ting JP, O’Neill LA. A role for the NLRP3 inflammasome in metabolic diseases--did Warburg miss inflammation? Nat Immunol 2012;13:352–7. [CrossRef ]
20. Li T, Xu Y, Liu L, Huang M, Wang Z, Tong Z, et al. Brucella Melitensis 16M Regulates the Effect of AIR Domain on Inflammatory Factors, Autophagy, and Apoptosis in Mouse Macrophage through the ROS Signaling Pathway. PLoS One 2016;11:e0167486. [CrossRef ]
21. Pétrilli V, Papin S, Dostert C, Mayor A, Martinon F, Tschopp J. Activation of the NALP3 inflammasome is triggered by low intracellular potassium concentration. Cell Death Differ 2007;14:1583–9. [CrossRef ]
22. Zhou Y, Zhao D, Yue R, Khan SH, Shah SZ, Yin X, et al. Inflammasomes-dependent regulation of IL-1β secretion induced by the virulent Mycobacterium bovis Beijing strain in THP-1 macrophages. Antonie Van Leeuwenhoek 2015;108:163–71. [CrossRef ]
23. Hayashi F, Smith KD, Ozinsky A, Hawn TR, Yi EC, Goodlett DR, et al. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature 2001;410:1099–103. [CrossRef ]
24. van de Veerdonk FL, Netea MG, Dinarello CA, Joosten LA. Inflammasome activation and IL-1β and IL-18 processing during infection. Trends Immunol 2011;32:110–6. [CrossRef ]
25. Bergsbaken T, Fink SL, Cookson BT. Pyroptosis: host cell death and inflammation. Nat Rev Microbiol 2009;7:99–109. [CrossRef ]
26. Terwagne M, Ferooz J, Rolán HG, Sun YH, Atluri V, Xavier MN, et al. Innate immune recognition of flagellin limits systemic persistence of Brucella. Cell Microbiol 2013;15:942–60. [CrossRef ]

APA	KARACA G, karaca z, KAYHAN B, BAYINDIR Y, Kayabas U, ALTUNIŞIK TOPLU S, elmasdag s, ETEM E, yesilada e (2019). The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis. , 117 - 123. 10.14744/nci.2018.96992
Chicago	KARACA Gamze,karaca zeynal mete,KAYHAN BASAK,BAYINDIR Yasar,Kayabas Uner,ALTUNIŞIK TOPLU SİBEL,elmasdag sirvan,ETEM Ebru,yesilada elif The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis. (2019): 117 - 123. 10.14744/nci.2018.96992
MLA	KARACA Gamze,karaca zeynal mete,KAYHAN BASAK,BAYINDIR Yasar,Kayabas Uner,ALTUNIŞIK TOPLU SİBEL,elmasdag sirvan,ETEM Ebru,yesilada elif The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis. , 2019, ss.117 - 123. 10.14744/nci.2018.96992
AMA	KARACA G,karaca z,KAYHAN B,BAYINDIR Y,Kayabas U,ALTUNIŞIK TOPLU S,elmasdag s,ETEM E,yesilada e The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis. . 2019; 117 - 123. 10.14744/nci.2018.96992
Vancouver	KARACA G,karaca z,KAYHAN B,BAYINDIR Y,Kayabas U,ALTUNIŞIK TOPLU S,elmasdag s,ETEM E,yesilada e The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis. . 2019; 117 - 123. 10.14744/nci.2018.96992
IEEE	KARACA G,karaca z,KAYHAN B,BAYINDIR Y,Kayabas U,ALTUNIŞIK TOPLU S,elmasdag s,ETEM E,yesilada e "The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis." , ss.117 - 123, 2019. 10.14744/nci.2018.96992
ISNAD	KARACA, Gamze vd. "The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis". (2019), 117-123. https://doi.org/10.14744/nci.2018.96992

APA	KARACA G, karaca z, KAYHAN B, BAYINDIR Y, Kayabas U, ALTUNIŞIK TOPLU S, elmasdag s, ETEM E, yesilada e (2019). The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis. İstanbul Kuzey Klinikleri, 6(2), 117 - 123. 10.14744/nci.2018.96992
Chicago	KARACA Gamze,karaca zeynal mete,KAYHAN BASAK,BAYINDIR Yasar,Kayabas Uner,ALTUNIŞIK TOPLU SİBEL,elmasdag sirvan,ETEM Ebru,yesilada elif The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis. İstanbul Kuzey Klinikleri 6, no.2 (2019): 117 - 123. 10.14744/nci.2018.96992
MLA	KARACA Gamze,karaca zeynal mete,KAYHAN BASAK,BAYINDIR Yasar,Kayabas Uner,ALTUNIŞIK TOPLU SİBEL,elmasdag sirvan,ETEM Ebru,yesilada elif The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis. İstanbul Kuzey Klinikleri, vol.6, no.2, 2019, ss.117 - 123. 10.14744/nci.2018.96992
AMA	KARACA G,karaca z,KAYHAN B,BAYINDIR Y,Kayabas U,ALTUNIŞIK TOPLU S,elmasdag s,ETEM E,yesilada e The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis. İstanbul Kuzey Klinikleri. 2019; 6(2): 117 - 123. 10.14744/nci.2018.96992
Vancouver	KARACA G,karaca z,KAYHAN B,BAYINDIR Y,Kayabas U,ALTUNIŞIK TOPLU S,elmasdag s,ETEM E,yesilada e The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis. İstanbul Kuzey Klinikleri. 2019; 6(2): 117 - 123. 10.14744/nci.2018.96992
IEEE	KARACA G,karaca z,KAYHAN B,BAYINDIR Y,Kayabas U,ALTUNIŞIK TOPLU S,elmasdag s,ETEM E,yesilada e "The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis." İstanbul Kuzey Klinikleri, 6, ss.117 - 123, 2019. 10.14744/nci.2018.96992
ISNAD	KARACA, Gamze vd. "The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis". İstanbul Kuzey Klinikleri 6/2 (2019), 117-123. https://doi.org/10.14744/nci.2018.96992