TY  - JOUR
TI  - The cytotoxic properties and apoptotic potential of N-butyl and 2-octyl cyanoacrylates used in surgical treatment of chronic venous insufficiency
AB  - Background: This study aims to investigate the cytotoxic effects andapoptotic potential of N-butyl cyanoacrylate and 2-octyl cyanoacrylateused in surgical treatment of chronic venous insufficiency.Methods: N-butyl cyanoacrylate and 2-octyl cyanoacrylate werecultured in cell-culture using human umbilical endothelial cell-line.Cytotoxicity and viability were assessed at 24 and 72 hours with lactatedehydrogenase and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assays, respectively. Apoptotic potential was documented at24 and 72 hours with relative caspase-3 activity.Results: The mean cytotoxicity at 24 and 72 hours were: N-butylcyanoacrylate with an area of dot/line: 37.0±3.9%/29.3±2.7% and46.4±1.6%/45.1±7.1%, 2-octyl cyanoacrylate with an area of dot/line:39.0±7.0%/37.3±4.6% and 47.0±2.3%/40.7±7.5%. Cytotoxicity increasedby time in each group (p<0.05). The mean viability at 24 and 72 hours were:N-butyl cyanoacrylate with an area of dot/line: 53.4±7.7%/72.0±5.7%and 35.7±1.9%/37.8±3.7%, 2-octyl cyanoacrylate with an area ofdot/line: 54.3±4.4%/73.5±19.9% and 33.6±2.8%/30.7±4.5%. The meanviability decreased by time in each group (p<0.05). The mean relativecaspase-3 activity at 24 and 72 hours were: control group: 0.084±0.006and 0.065±0.002, N-butyl cyanoacrylate with an area of dot/line:0.940±0.037/0.924±0.053 and 0.999±0.072/1.056±0.015, 2-octylcyanoacrylate with an area of dot/line: 0.900±0.044/0.928±0.018 and0.989±0.084/0.999±0.072. The mean relative caspase-3 activity washigher than control group in each group at each time interval (p<0.05)and activity increased by time in N-butyl cyanoacrylate line and in2-octyl cyanoacrylate line groups (p<0.05).Conclusion: Our findings indicate that N-butyl cyanoacrylate and2-octyl cyanoacrylate cause cytotoxicity in cell-culture media. We mayalso postulate that they induce apoptosis in cell-culture media.
AU  - ÖZDEMİR, AYŞE
AU  - AKBAY, Esin
AU  - Ünlü, Ahmet
AU  - ONUR, MEHMET ALİ
AU  - Durukan, Ahmet Baris
DO  - 10.5606/tgkdc.dergisi.2019.17091
PY  - 2019
JO  - Türk Göğüs Kalp Damar Cerrahisi Dergisi
VL  - 27
IS  - 2
SN  - 1301-5680
SP  - 185
EP  - 191
DB  - TRDizin
UR  - http://search/yayin/detay/338616
ER  -