Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders
Yıl: 2019 Cilt: 25 Sayı: 2 Sayfa Aralığı: 251 - 256 Metin Dili: İngilizce DOI: 10.9775/kvfd.2018.20819 İndeks Tarihi: 03-06-2020
Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders
Öz: Bovine respiratory disease (BRD) causes economic losses related to a reduction in weight gain of affected animals, veterinary treatment costs,death, etc. One of the important respiratory tract disease viruses is the bovine respiratory syncytial virus (BRSV). In this study, it is aimed toreport the molecular characterization of detected BRSVs. Therefore, nasal samples from three calves in a herd seen severe respiratory disorderswere examined for BRSV and other possible viral etiological agents by PCRs and the amplicons were sequenced. In the phylogenetic tree,BRSV circulating in this herd is clustered with the genetic subgroup III BRSVs deposited in GenBank from some other countries. This study onthe molecular characterization of BRSV circulating in calves would contribute for future studies on the epidemiology of this infection and thedevelopment and/or choice the effective vaccines in Turkey.
Anahtar Kelime: Solunum Sistemi Hastalığı Olan Buzağılarda Saptanan Bovine Respiratory Syncytial Virusun Filogenetik Analizi
Öz: Sığırlarda solunum sistemi hastalıkları, etkilenen hayvanların kilo alımında azalma, veteriner tedavi masrafları, ölüm gibi sebeplerle ekonomik kayıplara neden olmaktadır. Bovine Respiratory Syncytial Virus (BRSV), solunum sistemini etkileyen viral etkenlerin başında gelmektedir. Bu çalışmada BRSV’nin saptanması ve tespit edilen etkenlerin moleküler karakterizasyonlarının ortaya konulması amaçlanmıştır. Bu amaçla, buzağılarda ağır solunum yolu sistemi bulguları gözlenen bir işletmedeki üç buzağıdan alınan burun akıntısı örnekleri BRSV ve diğer viral etkenler yönünden PCR ile test edilmiştir. RT-PCR sonucunda pozitif bulunan örneklerin dizin bilgileri elde edilmiştir. Yapılan filogenetik analiz sonucunda bu sürüde tespit edilen BRSV’nin genetik olarak subgrup III içerisinde yer aldığı ortaya konulmuştur. Buzağılarda saptanan BRSV’nin moleküler karakterizasyonu üzerine yapılan bu çalışma, bu enfeksiyonun epidemiyolojisi ve etkili aşıların geliştirilmesi ve/veya seçimiyle ilgili gelecekteki çalışmalara katkıda bulunacaktır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Karayel-Hacioglu I, Coşkun N, DURAN YELKEN S, SEVİNÇ S, Alkan F (2019). Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders. , 251 - 256. 10.9775/kvfd.2018.20819 |
| Chicago | Karayel-Hacioglu Ilke,Coşkun Nüvit,DURAN YELKEN Selda,SEVİNÇ Seçil,Alkan Feray Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders. (2019): 251 - 256. 10.9775/kvfd.2018.20819 |
| MLA | Karayel-Hacioglu Ilke,Coşkun Nüvit,DURAN YELKEN Selda,SEVİNÇ Seçil,Alkan Feray Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders. , 2019, ss.251 - 256. 10.9775/kvfd.2018.20819 |
| AMA | Karayel-Hacioglu I,Coşkun N,DURAN YELKEN S,SEVİNÇ S,Alkan F Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders. . 2019; 251 - 256. 10.9775/kvfd.2018.20819 |
| Vancouver | Karayel-Hacioglu I,Coşkun N,DURAN YELKEN S,SEVİNÇ S,Alkan F Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders. . 2019; 251 - 256. 10.9775/kvfd.2018.20819 |
| IEEE | Karayel-Hacioglu I,Coşkun N,DURAN YELKEN S,SEVİNÇ S,Alkan F "Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders." , ss.251 - 256, 2019. 10.9775/kvfd.2018.20819 |
| ISNAD | Karayel-Hacioglu, Ilke vd. "Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders". (2019), 251-256. https://doi.org/10.9775/kvfd.2018.20819 |
| APA | Karayel-Hacioglu I, Coşkun N, DURAN YELKEN S, SEVİNÇ S, Alkan F (2019). Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25(2), 251 - 256. 10.9775/kvfd.2018.20819 |
| Chicago | Karayel-Hacioglu Ilke,Coşkun Nüvit,DURAN YELKEN Selda,SEVİNÇ Seçil,Alkan Feray Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 25, no.2 (2019): 251 - 256. 10.9775/kvfd.2018.20819 |
| MLA | Karayel-Hacioglu Ilke,Coşkun Nüvit,DURAN YELKEN Selda,SEVİNÇ Seçil,Alkan Feray Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.25, no.2, 2019, ss.251 - 256. 10.9775/kvfd.2018.20819 |
| AMA | Karayel-Hacioglu I,Coşkun N,DURAN YELKEN S,SEVİNÇ S,Alkan F Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2019; 25(2): 251 - 256. 10.9775/kvfd.2018.20819 |
| Vancouver | Karayel-Hacioglu I,Coşkun N,DURAN YELKEN S,SEVİNÇ S,Alkan F Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2019; 25(2): 251 - 256. 10.9775/kvfd.2018.20819 |
| IEEE | Karayel-Hacioglu I,Coşkun N,DURAN YELKEN S,SEVİNÇ S,Alkan F "Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25, ss.251 - 256, 2019. 10.9775/kvfd.2018.20819 |
| ISNAD | Karayel-Hacioglu, Ilke vd. "Phylogenetic Analysis of Bovine Respiratory Syncytial Virus from Calves with Respiratory Disorders". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 25/2 (2019), 251-256. https://doi.org/10.9775/kvfd.2018.20819 |
