Effect of xylitol on gut microbiota in an in vitro colonic simulation

AbstractObjective: Xylitol has been commonly used as a sweetener and dental caries protective agent. However, how xylitol influences the composition and metabolism of gut microbiota is not known yet. This study aimed to dissect the changes of microbiota and their metabolites under xylitol supplementation in an in vitro colonic simulation.Materials and methods: A single-phase continuous fermentation model was used to culture human fecal flora and the 16s rDNA and short chain fatty acid were analyzed.Results and discussion: It was found that gut microbiota composition differentiated after xylitol supplementation only for the beginning 3 days. Xylitol significantly enhanced the relative amount of butyrate synthesizing bacteria such as Clostridium and Phascolarctobacterium. Meanwhile, xylitol increased the production of propionicacid and butyrate. An increase of Escherichia population sizes after xylitol supplementation was beyond expectation. By Spearman analysis, a positive relationship between Escherichia and Bifidobacterium was found Conclusion: xylitol can rapidly enhance the total amount of short chain fatty acids, but its influence will disappear after 3 days of fermentation. Results of this investigation can be a guideline for the further investigations on xylitol in relation to gut microbiota and the daily intake determinations.

Anahtar Kelime:

Ksilitolün Bagirsakta Mikrobiyota Üzerine Etkisi in vitro kolonik simülasyon

Öz:

Öz Amaç: Ksilitol yaygın olarak bir tatlandırıcı ve diş çürüğü koruyucu madde olarak kullanılmıştır. Bununla birlikte, ksilitolün bağırsak mikrobiyotasının bileşimi ve metabolizmasını nasıl etkilediği henüz bilinmemektedir. Bu çalışma, mikrobiyota ve metabolitlerinin ksilitol desteği altındaki metabolitlerinin in vitro kolonik simülasyonda değişmesini amaçlamaktadır.Gereç ve Yöntem: İnsan fekal florasını kültürlemek için tek fazlı sürekli bir fermantasyon modeli kullanıldı ve 16’ların rDNA’sı ve kısa zincirli yağ asidi analiz edildi.Bulgular ve Tartışma: Bağırsak mikrobiyota kompozisyonunun ksilitol takviyesi sonrasında sadece üç gün için farklılaştığı bulundu. Ksilitol, Clostridium ve Phascolarctobacterium gibi göreceli butirat sentezleyici bakteri miktarını arttırmıştır. Bu arada, ksilitol, propiyonik asit ve bütirat üretimini arttırdı. Ksilitol takviyesi sonrası Escherichia popülasyon büyüklüğündeki artış beklentinin ötesindedir. Spearman analizi ile Escherichia ve Bifidobacterium arasında pozitif bir ilişki bulunmuştur.Sonuç: Ksilitol, toplam kısa zincirli yağ asidi miktarını hızlı bir şekilde arttırabilir, ancak etkisi üç günlük fermantasyondan sonra kaybolacaktır. Bu araştırmanın sonuçları, bağırsak mikrobiyotası ve günlük alım tayinleri ile ilgili olarak ksilitol ile ilgili daha ileri araştırmalar için bir rehber olabilir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	XU Y, CHEN Y, XIANG S, YE K, BAO X, Zhu X, GE Y, SHI L, LİN M (2019). Effect of xylitol on gut microbiota in an in vitro colonic simulation. , 646 - 653. 10.1515/tjb-2018-0328
Chicago	XU Yuanyuan,CHEN Yi,XIANG Shasha,YE Kun,BAO Xuan,Zhu Xuan,GE Yin,SHI Lihua,LİN Mengyi Effect of xylitol on gut microbiota in an in vitro colonic simulation. (2019): 646 - 653. 10.1515/tjb-2018-0328
MLA	XU Yuanyuan,CHEN Yi,XIANG Shasha,YE Kun,BAO Xuan,Zhu Xuan,GE Yin,SHI Lihua,LİN Mengyi Effect of xylitol on gut microbiota in an in vitro colonic simulation. , 2019, ss.646 - 653. 10.1515/tjb-2018-0328
AMA	XU Y,CHEN Y,XIANG S,YE K,BAO X,Zhu X,GE Y,SHI L,LİN M Effect of xylitol on gut microbiota in an in vitro colonic simulation. . 2019; 646 - 653. 10.1515/tjb-2018-0328
Vancouver	XU Y,CHEN Y,XIANG S,YE K,BAO X,Zhu X,GE Y,SHI L,LİN M Effect of xylitol on gut microbiota in an in vitro colonic simulation. . 2019; 646 - 653. 10.1515/tjb-2018-0328
IEEE	XU Y,CHEN Y,XIANG S,YE K,BAO X,Zhu X,GE Y,SHI L,LİN M "Effect of xylitol on gut microbiota in an in vitro colonic simulation." , ss.646 - 653, 2019. 10.1515/tjb-2018-0328
ISNAD	XU, Yuanyuan vd. "Effect of xylitol on gut microbiota in an in vitro colonic simulation". (2019), 646-653. https://doi.org/10.1515/tjb-2018-0328

APA	XU Y, CHEN Y, XIANG S, YE K, BAO X, Zhu X, GE Y, SHI L, LİN M (2019). Effect of xylitol on gut microbiota in an in vitro colonic simulation. Türk Biyokimya Dergisi, 44(5), 646 - 653. 10.1515/tjb-2018-0328
Chicago	XU Yuanyuan,CHEN Yi,XIANG Shasha,YE Kun,BAO Xuan,Zhu Xuan,GE Yin,SHI Lihua,LİN Mengyi Effect of xylitol on gut microbiota in an in vitro colonic simulation. Türk Biyokimya Dergisi 44, no.5 (2019): 646 - 653. 10.1515/tjb-2018-0328
MLA	XU Yuanyuan,CHEN Yi,XIANG Shasha,YE Kun,BAO Xuan,Zhu Xuan,GE Yin,SHI Lihua,LİN Mengyi Effect of xylitol on gut microbiota in an in vitro colonic simulation. Türk Biyokimya Dergisi, vol.44, no.5, 2019, ss.646 - 653. 10.1515/tjb-2018-0328
AMA	XU Y,CHEN Y,XIANG S,YE K,BAO X,Zhu X,GE Y,SHI L,LİN M Effect of xylitol on gut microbiota in an in vitro colonic simulation. Türk Biyokimya Dergisi. 2019; 44(5): 646 - 653. 10.1515/tjb-2018-0328
Vancouver	XU Y,CHEN Y,XIANG S,YE K,BAO X,Zhu X,GE Y,SHI L,LİN M Effect of xylitol on gut microbiota in an in vitro colonic simulation. Türk Biyokimya Dergisi. 2019; 44(5): 646 - 653. 10.1515/tjb-2018-0328
IEEE	XU Y,CHEN Y,XIANG S,YE K,BAO X,Zhu X,GE Y,SHI L,LİN M "Effect of xylitol on gut microbiota in an in vitro colonic simulation." Türk Biyokimya Dergisi, 44, ss.646 - 653, 2019. 10.1515/tjb-2018-0328
ISNAD	XU, Yuanyuan vd. "Effect of xylitol on gut microbiota in an in vitro colonic simulation". Türk Biyokimya Dergisi 44/5 (2019), 646-653. https://doi.org/10.1515/tjb-2018-0328