The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury

Baylan, Hüseyin; ÇOMAKLI, Selim; Öztürk, Deniz; GÜZEL ERDOĞAN, Derya; POLAT, Elif; TANYELİ, Ayhan

doi:10.1515/tjb-2018-0286

The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury

Derya GÜZEL ERDOĞAN, (Sakarya University, Faculty of Medicine, Department of Physiology, Adapazarı/Sakarya, Turkey)

Deniz ÖZTÜRK, (Health Services Vocational School, Ataturk University, Erzurum, Turkey)

Ayhan TANYELİ, (Department of Physiology, Faculty of Medicine, Atatürk University, Erzurum, Turkey)

Selim ÇOMAKLI, (Department of Pathology, Faculty of Medicine, Atatürk University, Erzurum, Turkey)

Hüseyin BAYLAN, (Department of Anatomy, Faculty of Medicine, Sakarya University, Sakarya, Turkey)

Elif POLAT (Department of Biochemistry, Faculty of Medicine, Atatürk University, Erzurum, Turkey)

Türk Biyokimya Dergisi

5 1

Yıl: 2019 Cilt: 44 Sayı: 4 Sayfa Aralığı: 539 - 548 Metin Dili: İngilizce DOI: 10.1515/tjb-2018-0286 İndeks Tarihi: 06-06-2020

The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury

Öz:

Objective: The effects of urapidil in intestinal ischemiareperfusion (IR) model were investigated using histopathological and biochemical methods.Materials and methods: Forty Wistar albino rats weresubjected to sham operation (Group 1), IR (Group 2),IR + dimethyl sulfoxide (Group 3), IR + urapidil 0.5 mg/kg(Group 4), and IR + urapidil 5 mg/kg (Group 5). Levels ofMDA, TAS, TOS, SOD, MPO, NF-κB, caspase-3, and LC3Bwere measured.Results and discussion: The groups 2 and 3 had significantly higher TOS and MPO levels than the sham grouphad (p < 0.001), whereas the TAS and SOD levels weresignificantly lower in Group 2 than in the sham group.In treatment groups, TAS and SOD levels increased,whereas TOS, MPO, and MDA levels decreased comparedto Group 2. Caspase-3 and LC3B immunopositivities wereseen at severe levels in Group 2 and 3. However, Group 4and 5 were found to have lower levels of immunopositivity.Immunopositivity was observed in interstitial areas, peribronchial region, and bronchial epithelial cells. A moderate level of NF-κB immunopositivity was seen in Group 2and 3.Conclusion: Our results show that urapidil is one of theantioxidant agents and protects lung tissue from oxidanteffects of intestinal IR injury.

Anahtar Kelime:

İntestinal İskemi-Reperfüzyon Hasarı Sonrası Urapidil’in Akciğer Dokusu Üzerine Koruyucu Etkileri

Öz:

Amaç: İntestinal iskemi-reperfüzyon (IR) modelinde urapidil’in etkileri histopatolojik ve biyokimyasal yöntemler kullanılarak araştırıldı. Gereç ve Yöntem: Kırk adet Wistar albino sıçan sham operasyon (Grup 1), IR (Grup 2), IR + dimetil sülfoksit (Grup 3), IR + urapidil 0.5 mg/kg (Grup 4) ve IR + urapidil 5 mg/kg (Grup 5) olarak gruplandı. MDA, TAS, TOS, SOD, MPO, NF-κB, kaspaz-3 ve LC3B seviyeleri ölçüldü. Bulgular ve tartışma: Grup 2 ve 3’te sham grubuna göre TOS ve MPO düzeylerinin anlamlı derecede yüksek, (p < 0.001), TAS ve SOD düzeyleri grup 2’de sham grubundan anlamlı derecede düşüktü. Tedavi gruplarında TAS ve SOD seviyeleri yükselirken, TOS, MPO ve MDA düzeyleri Grup 2’ye göre arttı. Kaspaz-3 ve LC3B immünopozitivitesi Grup 2 ve 3’te yoğun seviyelerde görüldü İnterstisyel bölgelerde, peribronşiyal bölgede ve bronşiyal epitel hücrelerinde immünopozitivite gözlendi. Grup 2 ve 3’te orta düzeyde bir NF-kappa B immünopozitivitesi görüldü. Sonuç: Sonuçlarımız urapidil’in, antioksidan ajanlardan biri olduğu ve akciğer dokusunu bağırsak IR hasarının oksidan etkilerinden koruduğunu göstermiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	GÜZEL ERDOĞAN D, Öztürk D, TANYELİ A, ÇOMAKLI S, Baylan H, POLAT E (2019). The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury. , 539 - 548. 10.1515/tjb-2018-0286
Chicago	GÜZEL ERDOĞAN Derya,Öztürk Deniz,TANYELİ Ayhan,ÇOMAKLI Selim,Baylan Hüseyin,POLAT Elif The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury. (2019): 539 - 548. 10.1515/tjb-2018-0286
MLA	GÜZEL ERDOĞAN Derya,Öztürk Deniz,TANYELİ Ayhan,ÇOMAKLI Selim,Baylan Hüseyin,POLAT Elif The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury. , 2019, ss.539 - 548. 10.1515/tjb-2018-0286
AMA	GÜZEL ERDOĞAN D,Öztürk D,TANYELİ A,ÇOMAKLI S,Baylan H,POLAT E The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury. . 2019; 539 - 548. 10.1515/tjb-2018-0286
Vancouver	GÜZEL ERDOĞAN D,Öztürk D,TANYELİ A,ÇOMAKLI S,Baylan H,POLAT E The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury. . 2019; 539 - 548. 10.1515/tjb-2018-0286
IEEE	GÜZEL ERDOĞAN D,Öztürk D,TANYELİ A,ÇOMAKLI S,Baylan H,POLAT E "The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury." , ss.539 - 548, 2019. 10.1515/tjb-2018-0286
ISNAD	GÜZEL ERDOĞAN, Derya vd. "The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury". (2019), 539-548. https://doi.org/10.1515/tjb-2018-0286

APA	GÜZEL ERDOĞAN D, Öztürk D, TANYELİ A, ÇOMAKLI S, Baylan H, POLAT E (2019). The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury. Türk Biyokimya Dergisi, 44(4), 539 - 548. 10.1515/tjb-2018-0286
Chicago	GÜZEL ERDOĞAN Derya,Öztürk Deniz,TANYELİ Ayhan,ÇOMAKLI Selim,Baylan Hüseyin,POLAT Elif The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury. Türk Biyokimya Dergisi 44, no.4 (2019): 539 - 548. 10.1515/tjb-2018-0286
MLA	GÜZEL ERDOĞAN Derya,Öztürk Deniz,TANYELİ Ayhan,ÇOMAKLI Selim,Baylan Hüseyin,POLAT Elif The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury. Türk Biyokimya Dergisi, vol.44, no.4, 2019, ss.539 - 548. 10.1515/tjb-2018-0286
AMA	GÜZEL ERDOĞAN D,Öztürk D,TANYELİ A,ÇOMAKLI S,Baylan H,POLAT E The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury. Türk Biyokimya Dergisi. 2019; 44(4): 539 - 548. 10.1515/tjb-2018-0286
Vancouver	GÜZEL ERDOĞAN D,Öztürk D,TANYELİ A,ÇOMAKLI S,Baylan H,POLAT E The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury. Türk Biyokimya Dergisi. 2019; 44(4): 539 - 548. 10.1515/tjb-2018-0286
IEEE	GÜZEL ERDOĞAN D,Öztürk D,TANYELİ A,ÇOMAKLI S,Baylan H,POLAT E "The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury." Türk Biyokimya Dergisi, 44, ss.539 - 548, 2019. 10.1515/tjb-2018-0286
ISNAD	GÜZEL ERDOĞAN, Derya vd. "The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury". Türk Biyokimya Dergisi 44/4 (2019), 539-548. https://doi.org/10.1515/tjb-2018-0286