TY  - JOUR
TI  - Molecular detection of Bacillus anthracis: evaluation of the efficiency of DNA extraction and a novel dry PCR
AB  - Background: Due to recent increase in mailings of anthraxspores, the detection of bioweapons has gained a greatdeal of interest. This study aimed to investigate the yieldand purity of DNA obtained from spores and vegetativeforms of Bacillus anthracis for detection by conventional(wet) and dry (lyophilized) PCR methods.Materials and methods: Biosamples from stock solution were reconstituted to a concentration 108 cfu/mL followed by the spectrophotometric measurement of theyield and purity of acquired DNA. Twelve wet and 12 dryPCR studies of four various DNA dilution samples wereperformed for each three target gene (cap, pag, sap) ofB. anthracis.Results: Significant differences for both DNA yields andpurity were found between liquid-agar and liquid-sporesamples. No significant difference was observed betweenwet and dry PCR in concentration of 2.5 ng/μL DNA for allgene regions. PCR results of sap gene region with DNAconcentrations of 1.5 ng/μL and 0.9 ng/μL was foundstatistically significant in favor of conventional wet PCRmethod (p = 0.047 and p ≤ 0.001, respectively).Conclusion: It is concluded that B. anthracis cultivatedin liquid medium is more usable than vegetative or sporeform obtained on plate agar for proper DNA extraction.
AU  - Sezigen, Sermet
AU  - KENAR, LEVENT
AU  - OKTEM, Hüseyin
AU  - EYİSON, Koray
AU  - ORTATATLI, Mesut
DO  - 10.1515/tjb-2018-0292
PY  - 2019
JO  - Türk Biyokimya Dergisi
VL  - 44
IS  - 2
SN  - 1303-829X
SP  - 147
EP  - 152
DB  - TRDizin
UR  - http://search/yayin/detay/340577
ER  -