An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period
Yıl: 2019 Cilt: 16 Sayı: 1 Sayfa Aralığı: 11 - 22 Metin Dili: İngilizce DOI: 10.33462/jotaf.516734 İndeks Tarihi: 15-06-2020
An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period
Öz: In this study, Origanum onites was used for the production of tincture, a hydroalcholic extract, and manufacturing conditions were optimized to determine the processing factor variable levels to increase the final quality of samples. For this purpose, response surface methodology study was applied and Box Behnken design was used for the optimization. Three factors were selected namely liquid/solid ratio, ethanol concentration and storage period for the preparation of samples. Total phenolic content, antiradical activity and efficient of concentration (EC50) values were determined to evaluate the bioactivity of tincture samples. It was observed that the total phenolic content of the tincture samples was affected by ethanol concentration and liquid/solid ratio. Total phenolic contents of tincture samples were in the range of 964.1-7020.8 mg GAE/ L samples while the efficient concentration (EC50) ranged from 44.88 to 89.56. Increase of solid level increased the bioactivity values of samples, but this increment decreased the tincture yield significantly. Optimization results showed that the highest total phenolic content would be at 50% ethanol concentration, 6 ml ethanol level which means the liquid/solid ratio is 1:5 and 15 days storage for the tincture samples.
Anahtar Kelime: Origanum Onites Tentürü Üretim Şartlarının Tepki Yüzey Yöntemi ile Optimizasyonu: Sıvı/Katı Oranı, Etanol Konsantrasyonu ve Depolama Süresinin Etkisi
Öz: Bu çalışmada, bir hidroalkolik ekstrakt tipi olan tentür üretimi amacıyla Origanum onites bitkisi kullanılmış ve son ürün kalitesini artırmaya yönelik olarak üretim şartlarını optimize etmek üzere proses faktör seviyelerinin belirlenmesi amaçlanmıştır. Bu amaçla, optimizasyon işlemi için tepki yüzey yöntemi tabanlı Box-Behnken deneme tasarımından faydalanılmıştır. Örneklerin hazırlanması amacıyla sıvı/katı oranı, etanol konsantrasyonu ve depolama süresi faktör olarak seçilmiştir. Tentür örneklerinin biyoaktif özelliklerinin belirlenmesi amacıyla toplam fenolik madde miktarı, antiradikal kapasite ve EC50 değeri hesaplanmıştır.Sıvı/katı oranı ve etanol konsantrasyonunun örneklerin toplam fenolik madde miktarı üzerinden etkili olduğu görülmüştür. Örneklerin toplam fenolik madde değerleri 964.1-7020.8 mg GAE/ L, EC50 değerleri ise 44.88-89.56 aralığında değişim göstermiştir. Örnek konsantrasyonunun artışı toplam fenolik madde miktarını artırırken, bu artış tentür veriminde düşüşe yol açmıştır. Optimizasyon sonuçları en yüksek toplam fenolik madde miktarının, %50 etanol konsantrasyonunda, 1:5 katı/sıvı oranında, 15 günlük depolama süresi sonunda elde edilebileceğini göstermiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Kaplan M, YILMAZ M, USLU R, KÖPRÜ S, GÖZELLE H, MUHDEREM G (2019). An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period. , 11 - 22. 10.33462/jotaf.516734 |
| Chicago | Kaplan Mahmut,YILMAZ Mustafa Mücahit,USLU Rabia,KÖPRÜ Serap,GÖZELLE Hürmet,MUHDEREM Gamze An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period. (2019): 11 - 22. 10.33462/jotaf.516734 |
| MLA | Kaplan Mahmut,YILMAZ Mustafa Mücahit,USLU Rabia,KÖPRÜ Serap,GÖZELLE Hürmet,MUHDEREM Gamze An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period. , 2019, ss.11 - 22. 10.33462/jotaf.516734 |
| AMA | Kaplan M,YILMAZ M,USLU R,KÖPRÜ S,GÖZELLE H,MUHDEREM G An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period. . 2019; 11 - 22. 10.33462/jotaf.516734 |
| Vancouver | Kaplan M,YILMAZ M,USLU R,KÖPRÜ S,GÖZELLE H,MUHDEREM G An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period. . 2019; 11 - 22. 10.33462/jotaf.516734 |
| IEEE | Kaplan M,YILMAZ M,USLU R,KÖPRÜ S,GÖZELLE H,MUHDEREM G "An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period." , ss.11 - 22, 2019. 10.33462/jotaf.516734 |
| ISNAD | Kaplan, Mahmut vd. "An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period". (2019), 11-22. https://doi.org/10.33462/jotaf.516734 |
| APA | Kaplan M, YILMAZ M, USLU R, KÖPRÜ S, GÖZELLE H, MUHDEREM G (2019). An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period. Tekirdağ Ziraat Fakültesi Dergisi, 16(1), 11 - 22. 10.33462/jotaf.516734 |
| Chicago | Kaplan Mahmut,YILMAZ Mustafa Mücahit,USLU Rabia,KÖPRÜ Serap,GÖZELLE Hürmet,MUHDEREM Gamze An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period. Tekirdağ Ziraat Fakültesi Dergisi 16, no.1 (2019): 11 - 22. 10.33462/jotaf.516734 |
| MLA | Kaplan Mahmut,YILMAZ Mustafa Mücahit,USLU Rabia,KÖPRÜ Serap,GÖZELLE Hürmet,MUHDEREM Gamze An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period. Tekirdağ Ziraat Fakültesi Dergisi, vol.16, no.1, 2019, ss.11 - 22. 10.33462/jotaf.516734 |
| AMA | Kaplan M,YILMAZ M,USLU R,KÖPRÜ S,GÖZELLE H,MUHDEREM G An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period. Tekirdağ Ziraat Fakültesi Dergisi. 2019; 16(1): 11 - 22. 10.33462/jotaf.516734 |
| Vancouver | Kaplan M,YILMAZ M,USLU R,KÖPRÜ S,GÖZELLE H,MUHDEREM G An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period. Tekirdağ Ziraat Fakültesi Dergisi. 2019; 16(1): 11 - 22. 10.33462/jotaf.516734 |
| IEEE | Kaplan M,YILMAZ M,USLU R,KÖPRÜ S,GÖZELLE H,MUHDEREM G "An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period." Tekirdağ Ziraat Fakültesi Dergisi, 16, ss.11 - 22, 2019. 10.33462/jotaf.516734 |
| ISNAD | Kaplan, Mahmut vd. "An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Liquid/Solid Ratio, Ethanol Concentration and Storage Period". Tekirdağ Ziraat Fakültesi Dergisi 16/1 (2019), 11-22. https://doi.org/10.33462/jotaf.516734 |
