Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi
Yıl: 2019 Cilt: 16 Sayı: 3 Sayfa Aralığı: 348 - 361 Metin Dili: Türkçe DOI: 10.33462/jotaf.556256 İndeks Tarihi: 17-06-2020
Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi
Öz: Rhizobium radiobacter (=Agrobacterium tumefaciens) tarafından neden olunan kök uru (Taç gal) hastalığı dünya genelinde yaygın olarak yumuşak ve sert çekirdekli meyve ağaçlarının yanı sıra, bağ, sebze ve süs bitkilerinin en önemli hastalıklarından biridir. Hastalık özellikle elma dahil birçok meyve fidanlıklarında ve genç meyve bahçelerde urlu bitkilerde gelişme geriliği sonucu ürün kaybı şeklinde ortaya çıkan önemli düzeylerde ekonomik kayıplara neden olur. Hastalığın kontrolünde fidanların köklerinin dikim öncesi çeşitli kimyasallara daldırılması, toprak fumigasyonu, toprak solarizasyonu ve biyolojik mücadele yöntemleri kullanılmaktadır. Bu çalışmada elma köklerinden izole edilen epifit ve endofit bakterilerinin kök ur hastalığı etmenine karşı antagonistik etkinliği belirlenmiştir. Çalışmada elma ağaçlarının kök ve kök boğazı bölgelerinden 85 aday epifitik ve endofitik bakteri izolatı elde edilmiştir. Aday antagonist bakteri izolatların hastalık etmeni R. radiobacter’i baskılama yeteneği öncelikle in vitro ikili kültür testi ile belirlenmiştir. İkili kültür testlerinden elde edilen sonuçlara göre Pseudomonas, Pantoea, Serratia and Bacillus cinslerine dahil farklı türlere ait 12 izolat, besi ortamı üzerinde patojene karşı ortalama 5.0-27.3 mm çapında arasında değişen engelleme zonları oluşturmuştur. Patojen gelişiminin engellenmesi üzerine farklı düzeylerde antagonistik etkinlik gösteren Pseudomonas putida’nın 3 izolatı (1-4en, 1-12en, 1-13en) havuç dilimi üzerinde yarı in vivo etkinlik çalışmaları için seçilmiştir. Pseudomonas putida 1-4en izolatı diğer izolatlara kıyasla havuç dilimi üzerinde ur oluşumunu önemli düzeyde baskılamıştır. Bu çalışmadan elde edilen sonuçlar Pseudomonas putida izolatlarının Rhizobium radiobacter’in neden olduğu kök boğazı ur hastalığına karşı biyolojik mücadele ajanı olarak kullanılabilecek bir potansiyele sahip olduğunu göstermektedir.
Anahtar Kelime: Determination of Antagonistic Potential of Epiphytic and Endophytic Bacterial İsolates Against Apple Crown Gal Disease Agent Rhizobium radiobacter
Öz: Crown gal disease, caused by Rhizobium radiobacter (formerly known as Agrobacterium tumefaciens), is one of the most important bacterial plant disease of stone fruits, pome fruits, grape vine, vegetable and ornamental plants distributed worldwide. Disease causes considerable damage in nursery and young orchards of variety of plants including apple where grower may suffer serious economic losses as galled plants show growth reduction and yield. Different control measures have been used against crown gall as a dipping of rooted plants into chemicals, soil fumigation, soil solarization and biological control. The aim of this study was to determine biocontrol efficacies of epiphytic and endophytic bacteria, obtained from apple roots and crowns, against crown gall disease agent. Eighty five putative epiphytic and endophytic antagonist bacterial isolates were obtained from the apple rhizosphere, roots and crown. By using dual culture test, putative antagonist bacterial isolates were screened for their ability to suppress disease agent R. radiobacter in vitro conditions. According to the results obtained from the preliminary dual culture test, 12 isolates, belonging to different bacterial species of Pseudomonas, Pantoea, Serratia and Bacillus genus were found to cause clear inhibition zones of 5.0-27.3 mm diameter. Among the tested antagonist isolates, three different bacterial isolates of Pseudomonas putida 1-4en, 1-12en and 1-13en were then chosen for semi in vivo studies which were conducted on carrot slice assay. Among these isolates, the most efficient bacterial isolate 1-14en significantly suppressed gall formation on carrot slices. Results of this study revealed that antagonist bacterial isolates of Pseudomonas putida has potential as biocontrol agent for crown gall disease caused by Rhizobium radiobacter.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Agrios, G.N. (2005). Plant Pathology. 5th Edition, Elsevier Academic Press, Amsterdam 952 p.
- Anand A., Mysore K.S. (2007) Agrobacterium biology and crown gall disease. In: Plant-Associated Bacteria. Gnanamanickam S.S. (Ed.). Springer, Dordrecht. pp. 359-384
- Anonymous, (2017). FAOSTAT, Food and Agriculture Organization of The United Nations (FAO). http://www.fao.org.tr (Erişim tarihi: 14.04.2019).
- Aysan, Y., Şahin, F. (2003). An outbreak of crown gall disease on rose caused by Agrobacterium tumefaciens in Turkey. Plant Pathology 52: 780.
- Aysan, Y., Şahin, F., Mirik, M., Dönmez, M., F., Tekman, H. (2003a). First report of crown gall of apricot (Prunus armeniaca) caused by Agrobacterium tumefaciens in Turkey. Plant Pathology 52: 793.
- Aysan, Y., A. Karatas and O. Cinar, 2003b. Biological control of bacterial stem rot caused by Erwinia chrysanthemi on tomato. Crop Protection 22 (6) 807-811. Berg, G., Krechel, A., Ditz, M., Sikora, RA., Ulrich, A., Hallmann, J. (2005). Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi. FEMS Microbiology Ecology 51: 215-229.
- Bozkurt, İ.A., Soylu, S. (2011). Hatay ili elma bahçelerinde Agrobacterium tumefaciens’in neden olduğu kök boğazı uru hastalığının belirlenmesi. IV. Bitki Koruma Kongresi. Bildiriler, 28-30 Haziran, Kahramanmaraş, s. 315.
- Bozkurt, İ.A., Soylu, S., (2016). Determination of effects of Plant Growth Promoting Rhizobacteria (PGPR) on seed germination and shoot growth of parsley (Petroselinum crispum L.). Turkey 6th Plant Protection Congress with International Participation (5-8 September 2016, Konya, TURKEY): 149.
- Coutinho, B.G., Licastro, D., Mendonc¸ Previato, L., Cámara, M., Venturi, V. (2015). Plant-influenced gene expression in the rice endophyte Burkholderia kururiensis M130. Molecular Plant-Microbe Interactions 28: 10–21.
- Farrand, S.K. (1990). Agrobacterium radiobacter strain K84: a model control system. In: Ralph R. Baker, Peter E. Dunn (eds) New Directions in Biological Control: Alternatives for Suppressing Agricultural Pests and Diseases, pp. 679-691.
- Garcia, F.A.D., Romeiro, R.D. (2011). Biocontrol of bean angular leaf spot by bacterial antagonists. Pesquisa Agropecuaria Brasileira 46: 1603-1608.
- Gerami, E., Hassanzadeh, N., Abdollahi, H., Ghasemi, A., Heydari, A. (2013). Evaluation of some bacterial antagonists for biological control of fire blight disease. Journal of Plant Pathology 95: 127-134.
- Gupta, A.K., Kamal, B. (2006). Pre-planting application of soil sterilents and herbicides for management of crown gall disease on ‘colt’ cherry rootstock. Indian Journal of Agricultural Sciences 76: 426–429.
- Gupta, A.K., Khosla, K. (2007). Integration of soil solarization and potential native antagonist for the management of crown gall on cherry rootstock colt. Scientia Horticulturae 112: 51-57.
- Gupta G., Parihar S.S., Ahirwar N.K., Snehi S.K., Singh V. (2015). Plant growth promoting rhizobacteria (PGPR): Current and future prospects for development of sustainable agriculture. Journal of Microbial and Biochemical Technology 7: 096-102.
- Hallmann, J., Quadt-Hallmann, A., Mahaffee, W.F., Kloepper, J.W. (1997). Bacterial endophytes in agricultural crops. Canadian Journal of Microbiology 43: 895-914.
- Horuz, S., Çağlar, B.K,. Kusek, M., Aysan, Y. (2018). Crown gall disease susceptibility of some stone fruit rootstocks in Turkey. Tarım Bilimleri Dergisi 24:439-444.
- Janse J.D. (1991). Pathovar discrimination within Pseudomonas savastanoi subsp. savastanoi using whole cell fatty acid analysis and pathogenicity as criteria. Systematic and Applied Microbiology 14: 79-84.
- Juniper, B.E., Watkins, R. and Harris, S.A. (1998). The origin of the apple. Acta Horticulture 484: 27-34.
- Kado, C. I. (2002). Crown gall. http://www.apsnet.org/ edcenter/Pages/phi.aspx (Erişim tarihi: 10.04.2019).
- Kara, M., Soylu, E.M., Kurt, Ş., Soylu, S. (2016). Determination of antagonistic efficiency of endophytic bacteria against gray mold disease agent Botrytis cinerea in vitro conditions. Turkey 6th Plant Protection Congress with International Participation (5-8 September 2016 Konya, TURKEY): p. 156
- Karaca, İ. (1966). Sistematik Bitki Hastalıkları (Bakteriyel Hastalıklar) Cilt 1. 210s.
- Kawaguchi, A., Kondo, K., Inoue, K. (2012). Biological control of apple crown gall by nonpathogenic Rhizobium vitis strain VAR03-1. Journal of General Plant Pathology 78: 287-293.
- Kheirandish, Z., Harighi, B. (2015). Evaluation of bacterial antagonists of Ralstonia solanacearum, causal agent of bacterial wilt of potato. Biological Control 86: 14-19.
- Khmel, I.A., Sorokina, T.A., Lemanova, N.B., Lipasova, V.A., Metlitski, O.Z,, Burdeinaya, T.V., Chernin, L.S. (1998). Biological control of crown gall in grapevine and raspberry by two Pseudomonas spp. with a wide spectrum of antagonistic activity. Biocontrol Science and Technology 8: 45-57. Kim, H., Nishiyama, M, Kunito T, Senoo K, Kawahara K, Murakami K, Oyaizu, H. (1998). High population of Sphingomonas species on plant surface. Journal of Applied Microbiology 85: 731–736.
- Küsek, M., Çınar Ö. (2012). Bitki büyümesini teşvik eden kök bakterilerini kullanılarak asma uru hastalığı etmeni Agrobacterium vitis’in biyolojik mücadelesi. Türkiye Biyolojik Mücadele Dergisi 3: 21-36.
- Lelliot, R.A., Stead, D.E. (1987). Methods for the diagnosis of bacterial diseases of plants. In: Methods in Plant Pathology, T.F. Preece, (Ed.). Black well Scientific Publications, Oxford. pp, 176-177.
- Lippincott, J.A., Lippincott, B.B. and Starr, M.P. ( 1981) The genus Agrobacterium. In: The Prokaryotes. M.P. Starr, H. Stolp, H.G. Trup, A. Balows and H.G. Schegel (Eds.). Springer‐Verlag, New York. Pp, 842– 845.
- López, M.M., Gorris, M.T., Salcedo, C.I., Montojo, A.M., Miró, M. (1989). Evidence of biological control of Agrobacterium tumefaciens strains sensitive and resistant to agrocin 84 by different Agrobacterium radiobacter strains on stone fruit trees. Applied and Environmental Microbiology 55: 741-746.
- Lopez-Reyes, J.G., Gilardi, G., Garibaldi, A., Gullino, M.L. (2014). Effıcacy of bacterıal and fungal bıocontrol agents as seed treatments against Fusarıum oxysporum f. sp lactucae on lettuce. Journal of Plant Pathology 96: 535-539.
- Matilla, M.A., Krell, T. (2018). Plant growth promotion and biocontrol mediated by plant-associated bacteria. In: Plant Microbiome: Stress response, Microorganisms for Sustainability 5, D. Egamberdieva and P. Ahmad (eds.), Springer Nature Singapore Pte Ltd. pp 45-80.
- Moore, L,W., Warren, G., (1979). Agrobacterium radiobacter strain 84 and biological control of crown gall. Annual Review of Phytopathology 17:163-179
- Moore, L,W., Canfield, M., (1996). Biology of Agrobacterium and management of crown gall disease. In: Principles and Practice of Managing Soilborne Plant Pathogens, R. Hall (Ed.). APS Press, St. Paul, Minnesota. pp, 151-191.
- Moore, L.W., Bouzar, H., Burr, T.J. ( 2001) Agrobacterium. In: Plant Pathogenic Bacteria. N.W. Schaad, J.B. Jones and W. Chun (Ed.). APS Press, St. Paul, Minnesota. pp, 17– 34.
- Penyalver, R., Lopez, M.M. (1999). Colonization of the rhizosphere by pathogenic Agrobacterium strains and nonpathogenic strains K84 and K1026, used for crown gall biocontrol. Applied and Environmental Microbiology 65: 1936–1940.
- Penyalver, R., Vicedo, B., Lopez, M.M. (2000). Use of genetically engineered Agrobacterium strain K1026 for biological control of crown gall. European Journal of Plant Pathology 106: 801-810.
- Rhouma, A., Bouri, M., Boubaker, A., Nesme, X. (2008). Potential effect of rhizobacteria in the management of crown gall dısease caused by Agrobacterıum tumefaciens Biovar 1. Journal of Plant Pathology 90: 517-526.
- Romero, F.M., Marina, M., Pieckenstain, F.L. (2014). The communities of tomato (Solanum lycopersicum L.) leaf endophytic bacteria, analyzed by 16S-ribosomal RNA gene pyrosequencing. FEMS Microbiology Letters 351: 187-194.
- Rosenbluet, M,. Martinez-Romero, E. (2006). Bacterial endophytes and their interactions with hosts. Molecular Plant-Microbe Interactions 19, 827–837.
- Santoyo G., Moreno-Hagelsieb, G, Carmen Orozco-Mosquedac, M, Glick, B.R. (2016). Plant growth-promoting bacterial endophytes. Microbiological Research 183: 92–99
- Soylu, S., Soylu, E.M., Kurt, Ş., Ekici, Ö.K. (2005). Antagonistic potentials of rhizosphere-associated bacterial isolates against soil-borne diseases of tomato and pepper caused by Sclerotinia sclerotiorum and Rhizoctonia solani. Pakistan Journal of Biological Sciences 8: 4348.
- Soylu, S., Kara, M., Üremiş, İ., Kurt, Ş., Soylu, E.M., Uysal, A., (2018). Determination of plant growth promoting traits of bacterial endophytes isolated and identified from invasive plant water hyacinth Eichhornia crassipes in Orontes river of Turkey. 1. International Mediterranean Symposium (01-03 November 2018, Mersin/Turkey): 349-350
- Stockwell, V.O., Kawalek, M.D., Moore, L.W., Loper, J.E. (1996). Transfer of pAgK84 from the biocontrol agent Agrobacterium radiobacter K84 to A. tumefaciens under field conditions. Phytopathology 86: 31–37.
- Sturz, A.V., Christie, B.R. Nowak, J. (2000). Bacterial endophytes, potential role in developing sustainable systems of crop production. Critical Reviews in Plant Sciences 19: 1-30.
- Sülü, S.M., Bozkurt, İ.A. Soylu, S. (2016). Bitki büyüme düzenleyici ve biyolojik mücadele etmeni olarak bakteriyel endofitler. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi 21: 103-111.
- Tabarraei, M., Amini, J., Harighi, B. (2011). Effects of fluorescent Pseudomonads for control of damping-off disease of cantaloupe caused by Phytophthora drechsleri. Australian Journal of Crop Science 5: 1427-1433.
- Tajalipour, S., Hassanzadeh, N., Jolfaee, H.K., Heydari, A., Ghasemi, A. (2014). Biological control of mushroom brown blotch disease using antagonistic bacteria. Biocontrol Science and Technology 24: 473-484.
- Ullah, A., Mushtaq, H., Fahad, S., Shah, A. Chaudhary, H.J. (2017). Plant growth promoting potential of bacterial endophytes in novel association with Olea ferruginea and Withania coagulans. Microbiology 86: 119-127.
- Van Loon L.C., Bakker, P.A.H.M., Pieterse, C.M.J., 1998. Systemic resistance induced by rhizosphere bacteria. Annual Review of Phytopathology 36: 483-553. Vessey, K.J. (2003). Plant growth promoting rhizobacteria as biofertilizers, Plant and Soil 255: 571–586.
- Yüzbaşıoğlu, E.G. (2014). Farklı konukçu bitkilerden izole edilen Agrobacterium tumefaciens izolatlarının fenotipik ve genotipik karakterizasyonu. Doktora Tezi, Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı, 139 s.
| APA | BOZKURT İ, SOYLU S (2019). Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi. , 348 - 361. 10.33462/jotaf.556256 |
| Chicago | BOZKURT İ..Adem,SOYLU Soner Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi. (2019): 348 - 361. 10.33462/jotaf.556256 |
| MLA | BOZKURT İ..Adem,SOYLU Soner Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi. , 2019, ss.348 - 361. 10.33462/jotaf.556256 |
| AMA | BOZKURT İ,SOYLU S Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi. . 2019; 348 - 361. 10.33462/jotaf.556256 |
| Vancouver | BOZKURT İ,SOYLU S Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi. . 2019; 348 - 361. 10.33462/jotaf.556256 |
| IEEE | BOZKURT İ,SOYLU S "Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi." , ss.348 - 361, 2019. 10.33462/jotaf.556256 |
| ISNAD | BOZKURT, İ..Adem - SOYLU, Soner. "Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi". (2019), 348-361. https://doi.org/10.33462/jotaf.556256 |
| APA | BOZKURT İ, SOYLU S (2019). Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi. Tekirdağ Ziraat Fakültesi Dergisi, 16(3), 348 - 361. 10.33462/jotaf.556256 |
| Chicago | BOZKURT İ..Adem,SOYLU Soner Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi. Tekirdağ Ziraat Fakültesi Dergisi 16, no.3 (2019): 348 - 361. 10.33462/jotaf.556256 |
| MLA | BOZKURT İ..Adem,SOYLU Soner Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi. Tekirdağ Ziraat Fakültesi Dergisi, vol.16, no.3, 2019, ss.348 - 361. 10.33462/jotaf.556256 |
| AMA | BOZKURT İ,SOYLU S Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi. Tekirdağ Ziraat Fakültesi Dergisi. 2019; 16(3): 348 - 361. 10.33462/jotaf.556256 |
| Vancouver | BOZKURT İ,SOYLU S Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi. Tekirdağ Ziraat Fakültesi Dergisi. 2019; 16(3): 348 - 361. 10.33462/jotaf.556256 |
| IEEE | BOZKURT İ,SOYLU S "Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi." Tekirdağ Ziraat Fakültesi Dergisi, 16, ss.348 - 361, 2019. 10.33462/jotaf.556256 |
| ISNAD | BOZKURT, İ..Adem - SOYLU, Soner. "Elma Kök Uru Hastalığı Etmeni Rhizobium radiobacter’e Karşı Epifit ve Endofit Bakteri İzolatlarının Antagonistik Potansiyellerinin Belirlenmesi". Tekirdağ Ziraat Fakültesi Dergisi 16/3 (2019), 348-361. https://doi.org/10.33462/jotaf.556256 |
