Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea)

Ayşenur EMİNOĞLU, (Recep Tayyip Erdoğan Üniversitesi, Biyoloji Bölümü, Moleküler Biyoloji Araştırma Laboratuvarları, Rize, Türkiye)
Yeşim AKTÜRK DİZMAN, (Recep Tayyip Erdoğan Üniversitesi, Biyoloji Bölümü, Moleküler Biyoloji Araştırma Laboratuvarları, Rize, Türkiye)
Şule GÜZEL, (Recep Tayyip Erdoğan Üniversitesi, Biyoloji Bölümü, Bitki Ekolojisi Araştırma Laboratuvarları, Rize, Türkiye)
Ali Osman BELDÜZ (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)
Turkish Journal of Biology
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Yıl: 2018 Cilt: 42 Sayı: 1 Sayfa Aralığı: 1 - 11 Metin Dili: İngilizce DOI: 10.3906/biy-1606-54 İndeks Tarihi: 08-07-2020

Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea)

Öz:
Cytochrome P450 monooxygenases are one of the largest heme-containing protein groups, and the majority of them catalyzehydroxylation reactions dependent on nicotinamide adenine dinucleotide phosphate and oxygen. Cytochrome P450 (CYP) enzymesfunction in a wide range of monooxygenation reactions essential in primary and secondary metabolism in plants. Camellia sinensis (L.)Kuntze is a commercially and economically valuable plant due to its medicinally important secondary metabolites and as a belovedbeverage. Cytochrome P450 monooxygenases play a significant role in the biosynthesis of a variety of secondary metabolites in tea.Although the biosynthesis of secondary metabolites has been investigated in detail, there have been limited studies conducted onidentifying the genetic mechanisms of CYP-catalyzed secondary metabolic pathways in the C. sinensis (tea) plant. In our study, wecharacterized a putative C. sinensis (L.) Kuntze cytochrome P450 monooxygenase gene (Csp450), which has 1759 bp full-length cDNAwith 49 bp of 5ʹ and 183 bp of 3ʹ untranslated regions. The CDS of the gene is 1527 bp and 508 amino acids in length. BLAST results ofthe deduced amino acid sequence revealed a high similarity with the CYP704C1-like superfamily. Preharvest period gene expressionanalysis from May, July, and September did not show any difference.
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APA EMİNOĞLU A, AKTÜRK DİZMAN Y, GÜZEL Ş, BELDÜZ A (2018). Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea). , 1 - 11. 10.3906/biy-1606-54
Chicago EMİNOĞLU Ayşenur,AKTÜRK DİZMAN YEŞİM,GÜZEL Şule,BELDÜZ Ali Osman Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea). (2018): 1 - 11. 10.3906/biy-1606-54
MLA EMİNOĞLU Ayşenur,AKTÜRK DİZMAN YEŞİM,GÜZEL Şule,BELDÜZ Ali Osman Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea). , 2018, ss.1 - 11. 10.3906/biy-1606-54
AMA EMİNOĞLU A,AKTÜRK DİZMAN Y,GÜZEL Ş,BELDÜZ A Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea). . 2018; 1 - 11. 10.3906/biy-1606-54
Vancouver EMİNOĞLU A,AKTÜRK DİZMAN Y,GÜZEL Ş,BELDÜZ A Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea). . 2018; 1 - 11. 10.3906/biy-1606-54
IEEE EMİNOĞLU A,AKTÜRK DİZMAN Y,GÜZEL Ş,BELDÜZ A "Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea)." , ss.1 - 11, 2018. 10.3906/biy-1606-54
ISNAD EMİNOĞLU, Ayşenur vd. "Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea)". (2018), 1-11. https://doi.org/10.3906/biy-1606-54
APA EMİNOĞLU A, AKTÜRK DİZMAN Y, GÜZEL Ş, BELDÜZ A (2018). Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea). Turkish Journal of Biology, 42(1), 1 - 11. 10.3906/biy-1606-54
Chicago EMİNOĞLU Ayşenur,AKTÜRK DİZMAN YEŞİM,GÜZEL Şule,BELDÜZ Ali Osman Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea). Turkish Journal of Biology 42, no.1 (2018): 1 - 11. 10.3906/biy-1606-54
MLA EMİNOĞLU Ayşenur,AKTÜRK DİZMAN YEŞİM,GÜZEL Şule,BELDÜZ Ali Osman Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea). Turkish Journal of Biology, vol.42, no.1, 2018, ss.1 - 11. 10.3906/biy-1606-54
AMA EMİNOĞLU A,AKTÜRK DİZMAN Y,GÜZEL Ş,BELDÜZ A Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea). Turkish Journal of Biology. 2018; 42(1): 1 - 11. 10.3906/biy-1606-54
Vancouver EMİNOĞLU A,AKTÜRK DİZMAN Y,GÜZEL Ş,BELDÜZ A Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea). Turkish Journal of Biology. 2018; 42(1): 1 - 11. 10.3906/biy-1606-54
IEEE EMİNOĞLU A,AKTÜRK DİZMAN Y,GÜZEL Ş,BELDÜZ A "Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea)." Turkish Journal of Biology, 42, ss.1 - 11, 2018. 10.3906/biy-1606-54
ISNAD EMİNOĞLU, Ayşenur vd. "Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea)". Turkish Journal of Biology 42/1 (2018), 1-11. https://doi.org/10.3906/biy-1606-54
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23-04-2024 12:34

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