TY  - JOUR
TI  - Molecular and in silico cloning, identification, and preharvest period expression analysis of a putative cytochrome P450 monooxygenase gene from Camellia sinensis (L.) Kuntze (tea)
AB  - Cytochrome P450 monooxygenases are one of the largest heme-containing protein groups, and the majority of them catalyzehydroxylation reactions dependent on nicotinamide adenine dinucleotide phosphate and oxygen. Cytochrome P450 (CYP) enzymesfunction in a wide range of monooxygenation reactions essential in primary and secondary metabolism in plants. Camellia sinensis (L.)Kuntze is a commercially and economically valuable plant due to its medicinally important secondary metabolites and as a belovedbeverage. Cytochrome P450 monooxygenases play a significant role in the biosynthesis of a variety of secondary metabolites in tea.Although the biosynthesis of secondary metabolites has been investigated in detail, there have been limited studies conducted onidentifying the genetic mechanisms of CYP-catalyzed secondary metabolic pathways in the C. sinensis (tea) plant. In our study, wecharacterized a putative C. sinensis (L.) Kuntze cytochrome P450 monooxygenase gene (Csp450), which has 1759 bp full-length cDNAwith 49 bp of 5ʹ and 183 bp of 3ʹ untranslated regions. The CDS of the gene is 1527 bp and 508 amino acids in length. BLAST results ofthe deduced amino acid sequence revealed a high similarity with the CYP704C1-like superfamily. Preharvest period gene expressionanalysis from May, July, and September did not show any difference.
AU  - BELDÜZ, Ali Osman
AU  - EMİNOĞLU, Ayşenur
AU  - AKTÜRK DİZMAN, YEŞİM
AU  - GÜZEL, Şule
DO  - 10.3906/biy-1606-54
PY  - 2018
JO  - Turkish Journal of Biology
VL  - 42
IS  - 1
SN  - 1300-0152
SP  - 1
EP  - 11
DB  - TRDizin
UR  - http://search/yayin/detay/349064
ER  -