Yıl: 2018 Cilt: 42 Sayı: 2 Sayfa Aralığı: 113 - 122 Metin Dili: İngilizce DOI: 10.3906/biy-1710-62 İndeks Tarihi: 09-07-2020

Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis

Öz:
MicroRNAs (miRNAs) are small noncoding RNAs of about 19–25 nt that regulate gene expression posttranscriptionally undervarious cellular conditions, including apoptosis. The miRNAs involved in modulation of apoptotic events in T cells are partially known.However, heterogeneity associated with cell lines makes it difficult to interpret gene expression signatures, especially in cancer-relatedcell lines. Treatment of the Jurkat T-cell leukemia cell line with the universal apoptotic drug, camptothecin, resulted in identification oftwo Jurkat subpopulations: one that is sensitive to camptothecin and another that is rather intrinsically resistant. We sorted apoptoticJurkat cells from nonapoptotic ones prior to profiling miRNAs through deep sequencing. Our data showed that a total of 184 miRNAswere dysregulated. Interestingly, the apoptotic and nonapoptotic subpopulations exhibited distinct miRNA expression profiles. Inparticular, 6 miRNAs were inversely expressed in these two subpopulations. The pyrosequencing results were validated by real-timeqPCR. Altogether, these results suggest that miRNAs modulate apoptotic events in T cells and that cellular heterogeneity requires carefulinterpretation of miRNA expression profiles obtained from drug-treated cell lines.
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APA ERDOGAN VATANSEVER I, COŞACAK M, NALBANT A, AKGÜL B (2018). Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. , 113 - 122. 10.3906/biy-1710-62
Chicago ERDOGAN VATANSEVER IPEK,COŞACAK Mehmet İlyas,NALBANT Ayten,AKGÜL Bünyamin Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. (2018): 113 - 122. 10.3906/biy-1710-62
MLA ERDOGAN VATANSEVER IPEK,COŞACAK Mehmet İlyas,NALBANT Ayten,AKGÜL Bünyamin Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. , 2018, ss.113 - 122. 10.3906/biy-1710-62
AMA ERDOGAN VATANSEVER I,COŞACAK M,NALBANT A,AKGÜL B Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. . 2018; 113 - 122. 10.3906/biy-1710-62
Vancouver ERDOGAN VATANSEVER I,COŞACAK M,NALBANT A,AKGÜL B Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. . 2018; 113 - 122. 10.3906/biy-1710-62
IEEE ERDOGAN VATANSEVER I,COŞACAK M,NALBANT A,AKGÜL B "Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis." , ss.113 - 122, 2018. 10.3906/biy-1710-62
ISNAD ERDOGAN VATANSEVER, IPEK vd. "Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis". (2018), 113-122. https://doi.org/10.3906/biy-1710-62
APA ERDOGAN VATANSEVER I, COŞACAK M, NALBANT A, AKGÜL B (2018). Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. Turkish Journal of Biology, 42(2), 113 - 122. 10.3906/biy-1710-62
Chicago ERDOGAN VATANSEVER IPEK,COŞACAK Mehmet İlyas,NALBANT Ayten,AKGÜL Bünyamin Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. Turkish Journal of Biology 42, no.2 (2018): 113 - 122. 10.3906/biy-1710-62
MLA ERDOGAN VATANSEVER IPEK,COŞACAK Mehmet İlyas,NALBANT Ayten,AKGÜL Bünyamin Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. Turkish Journal of Biology, vol.42, no.2, 2018, ss.113 - 122. 10.3906/biy-1710-62
AMA ERDOGAN VATANSEVER I,COŞACAK M,NALBANT A,AKGÜL B Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. Turkish Journal of Biology. 2018; 42(2): 113 - 122. 10.3906/biy-1710-62
Vancouver ERDOGAN VATANSEVER I,COŞACAK M,NALBANT A,AKGÜL B Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis. Turkish Journal of Biology. 2018; 42(2): 113 - 122. 10.3906/biy-1710-62
IEEE ERDOGAN VATANSEVER I,COŞACAK M,NALBANT A,AKGÜL B "Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis." Turkish Journal of Biology, 42, ss.113 - 122, 2018. 10.3906/biy-1710-62
ISNAD ERDOGAN VATANSEVER, IPEK vd. "Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis". Turkish Journal of Biology 42/2 (2018), 113-122. https://doi.org/10.3906/biy-1710-62