TY  - JOUR
TI  - Deep sequencing reveals two Jurkat subpopulations with distinct miRNA profiles during camptothecin-induced apoptosis
AB  - MicroRNAs (miRNAs) are small noncoding RNAs of about 19–25 nt that regulate gene expression posttranscriptionally undervarious cellular conditions, including apoptosis. The miRNAs involved in modulation of apoptotic events in T cells are partially known.However, heterogeneity associated with cell lines makes it difficult to interpret gene expression signatures, especially in cancer-relatedcell lines. Treatment of the Jurkat T-cell leukemia cell line with the universal apoptotic drug, camptothecin, resulted in identification oftwo Jurkat subpopulations: one that is sensitive to camptothecin and another that is rather intrinsically resistant. We sorted apoptoticJurkat cells from nonapoptotic ones prior to profiling miRNAs through deep sequencing. Our data showed that a total of 184 miRNAswere dysregulated. Interestingly, the apoptotic and nonapoptotic subpopulations exhibited distinct miRNA expression profiles. Inparticular, 6 miRNAs were inversely expressed in these two subpopulations. The pyrosequencing results were validated by real-timeqPCR. Altogether, these results suggest that miRNAs modulate apoptotic events in T cells and that cellular heterogeneity requires carefulinterpretation of miRNA expression profiles obtained from drug-treated cell lines.
AU  - ERDOGAN VATANSEVER, IPEK
AU  - AKGÜL, Bünyamin
AU  - COŞACAK, Mehmet İlyas
AU  - NALBANT, Ayten
DO  - 10.3906/biy-1710-62
PY  - 2018
JO  - Turkish Journal of Biology
VL  - 42
IS  - 2
SN  - 1300-0152
SP  - 113
EP  - 122
DB  - TRDizin
UR  - http://search/yayin/detay/349094
ER  -