TY  - JOUR
TI  - Polyphenolic contents of Teucrium polium L. and Teucrium scordium L. associated with their protective effects against MMC-induced chromosomal damage in cultured human peripheral blood lymphocytes
AB  - Teucrium species have been used in traditional medicine for treatment of different diseases. The aim of this study was toinvestigate polyphenolic contents by high-performance liquid chromatography (HPLC), and the genotoxic effect of methanolic extractsof Teucrium polium and Teucrium scordium using the cytokinesis-block micronucleus (CBMN) assay on human peripheral bloodlymphocytes (PBLs) from healthy donors. The HPLC analysis showed that extracts consist of phenolic acid (gallic, vanillic, caffeic,chlorogenic, p-coumaric, sinapic) and flavonoids (catechin, rutin, myricetin, luteolin, quercetin and apigenin). Cultures were treatedwith extracts of both plants separately and in combinations with mitomycin C (MMC). In separate treatments, both herbal extractssignificantly induced micronucleus (MN) frequency only at the highest concentrations. All concentrations of T. scordium, except thelowest, and all concentrations of T. polium extracts in combined treatment with MMC significantly reduced the frequency of MN.The extract of T. polium did not significantly affect the nuclear division index (NDI), whereas T. scordium in higher concentrations,separately and in combined treatment with MMC, significantly decreased the NDI value. Our results suggest that both herbal extracts incombination with MMC have antimutagenic (T. polium) and proapoptotic effects (T. scordium), which indicates their protective effectsin PBLs.
AU  - MARKOVIC, Aleksandra
AU  - STANKOVIC, Milan
AU  - RADOVIC JAKOVLJEVIC, Marina
AU  - MARINKOVIC, Dragoslav
AU  - MILOSEVIC DJORDJEVIC, Olivera
AU  - CIRIC, Andrija
AU  - GRUJICIC, Darko
DO  - 10.3906/biy-1707-36
PY  - 2018
JO  - Turkish Journal of Biology
VL  - 42
IS  - 2
SN  - 1300-0152
SP  - 152
EP  - 162
DB  - TRDizin
UR  - http://search/yayin/detay/349103
ER  -