A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study

Nisa GÜL AMUK, (Erciyes Üniversitesi, Diş Hekimliği Fakültesi, Ortodonti Anabilim Dalı, Kayseri, Türkiye)
Gökmen KURT, (Bezmiâlem Vakıf Üniversitesi, Diş Hekimliği Fakültesi, Ortodonti Anabilim Dalı, İstanbul, Türkiye)
Melis KARTAL YANDIM, (İzmir Ekonomi Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İzmir, Türkiye)
Aysun ADAN, (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)
Yusuf BARAN (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)
Turkish Journal of Biology
1 0
Yıl: 2018 Cilt: 42 Sayı: 5 Sayfa Aralığı: 382 - 391 Metin Dili: İngilizce DOI: 10.3906/biy-1712-62 İndeks Tarihi: 09-07-2020

A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study

Öz:
The aim of this study was to introduce a minimally invasive procedure for mesenchymal stem cell (MSC) transfer into theintact periodontal ligament (PDL) of the molar teeth in rats. Ten 12-week-old Wistar albino rats were used for this preliminary study.MSCs were obtained from bones of two animals and were labeled with green fluorescent protein (GFP). Four animals were randomlyselected for MSC injection, while 4 animals served as a control group. Samples were prepared for histological analysis, Cox-2 mRNAexpression polymerase chain reaction analysis, and fluorescent microscopy evaluation. The number of total cells, number of osteoclasticcells, and Cox-2 mRNA expression levels of the periodontal tissue of teeth were calculated. The number of total cells was increased withMSC injections in PDL significantly (P < 0.001). The number of osteoclastic cells and Cox-2 mRNA expression were found to be similarfor the two groups. GFP-labeled MSCs were observed with an expected luminescence on the smear samples of the PDL with transferredMSCs. The results of this preliminary study demonstrate successful evidence of transferring MSCs to intact PDL in a nonsurgical wayand offer a minimally invasive procedure for transfer of MSCs to periodontal tissues.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Achar S, Kundu S (2002). Principles of office anesthesia: part I. Infiltrative anesthesia. Am Fam Physician 66: 91-94.
  • Baba S, Yamada Y, Komuro A, Yotsui Y, Umeda M, Shimuzutani K, Nakamura S (2016). Phase I/II trial of autologous bone marrow stem cell transplantation with a three-dimensional wovenfabric scaffold for periodontitis. Stem Cells Int 2016: 6205910.
  • Beertsen W, McCulloch CA, Sodek J (1997). The periodontal ligament: a unique, multifunctional connective tissue. Periodontol 2000 13: 20-40.
  • Behnia H, Khojasteh A, Soleimani M, Tehranchi A, Atashia (2012). Repair of alveolar cleft defect with mesenchymal stem cells and platelet derived growth factors: a preliminary report. J Cranio Maxill Surg 40: 2-7.
  • Bright R, Hynes K, Gronthos S, Bartold P (2015). Periodontal ligament‐derived cells for periodontal regeneration in animal models: a systematic review. J Periodont Res 50: 160-172.
  • Cartier N, Hacein-Bey-Abina S, Bartholomae CC, Veres G, Schmidt M, Kutschera I, Vidaud M, Abel U, Dal-Cortivo L, Caccavelli L et al (2009). Hematopoietic stem cell gene therapy with a lentiviral vector in X-linked adrenoleukodystrophy. Science 326: 818-823.
  • Ekizer A, Yalvac E, Uysal T, Sonmez MF, Sahin F (2015). Bone marrow mesenchymal stem cells enhance bone formation in orthodontically expanded maxillae in rats. Angle Orthod 85: 394-399.
  • Falanga V, Iwamoto S, Chartier M, Yufit T, Butmarc J, Kouttab N, Shrayer D, Carson P (2007). Autologous bone marrow-derived cultured mesenchymal stem cells delivered in a fibrin spray accelerate healing in murine and human cutaneous wounds. Tissue Eng 13: 1299-1312.
  • Galili D, Kaufman E, Garfunkel A, Michaeli Y (1984). Intraligamentary anesthesia - a histological study. Int J Oral Surg 13: 511-516.
  • Garib DG, Henriques JF, Janson G, Roberto de Freitas M, Fernandes AY (2006). Periodontal effects of rapid maxillary expansion with tooth-tissue-borne and tooth-borne expanders: a computed tomography evaluation. Am J Orthod Dentofacial Orthop 129: 749-758.
  • Harada Y, Nakasa T, Mahmoud EE, Kamei G, Adachi N, Deie M, Ochi M (2015). Combination therapy with intra‐articular injection of mesenchymal stem cells and articulated joint distraction for repair of a chronic osteochondral defect in the rabbit. J Orthopaed Res 33: 1466-1473.
  • Hasegawa N, Kawaguchi H, Hirachi A, Takeda K, Mizuno N, Nishimura M, Koike C, Tsuji K, Iba H, Kato Y et al (2006). Behavior of transplanted bone marrow-derived mesenchymal stem cells in periodontal defects. J Periodontol 77: 1003-1007.
  • Hayman AR (2008). Tartrate-resistant acid phosphatase (TRAP) and the osteoclast/immune cell dichotomy. Autoimmunity 41: 218- 223.
  • Isaka J, Ohazama A, Kobayashi M, Nagashima C, Takiguchi T, Kawasaki H, Tachikawa T, Hasegawa K (2001). Participation of periodontal ligament cells with regeneration of alveolar bone. J Periodontol 72: 314-323.
  • Iyer SS, Rojas M (2008). Anti-inflammatory effects of mesenchymal stem cells: novel concept for future therapies. Expert Opin Biol Ther 8: 569-581.
  • Kawaguchi H, Hirachi A, Hasegawa N, Iwata T, Hamaguchi H, Shiba H, Takata T, Kato Y, Kurihara H (2004). Enhancement of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells. J Periodontol 75: 1281-1287.
  • Kramer P, Nares S, Kramer S, Grogan D, Kaiser M (2004). Mesenchymal stem cells acquire characteristics of cells in the periodontal ligament in vitro. J Dent Res 83: 27-34.
  • Lin NH, Gronthos S, Bartold PM (2009). Stem cells and future periodontal regeneration. Periodontol 2000 51: 239-251.
  • Liu Y, Zheng Y, Ding G, Fang D, Zhang C, Bartold PM, Grontos S, Shi S, Wang S (2008). Periodontal ligament stem cell‐mediated treatment for periodontitis in miniature swine. Stem Cells 26: 1065-1073.
  • Liu Z, Xu J, Wang D (2011). Ultrasound enhances the healing of orthodontically induced root resorption in rats. Angle Orthod 82: 48-55.
  • Murakami Y, Kojima T, Nagasawa T, Kobayashi H, Ishikawa I (2003). Novel isolation of alkaline phosphatase-positive subpopulation from periodontal ligament fibroblasts. J Periodontol 74: 780- 786.
  • Oyonarte R, Becerra D, Díaz-Zúñiga J, Rojas V, Flavio C (2013). Morphological effects of mesenchymal stem cells and pulsed ultrasound on condylar growth in rats: a pilot study. Aust Orthod J 29: 3-12.
  • Pihlstrom BL, Michalowicz BS, Johnson NW (2005). Periodontal diseases. Lancet 366: 1809-1820.
  • Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T (1995). Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr Biol 5: 635-642.
  • Seibert K, Zhang Y, Leahy K, Hauser S, Masferrer J, Perkins W, Lee L, Isakson P (1994). Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. P Natl Acad Sci USA 91: 12013-12037.
  • Takewaki M, Kajiya M, Takeda K, Sasaki S, Motoike S, Komatsu N, Matsuda S, Ouhara K, Mizuno N, Fujita T (2017). MSC/ECM cellular complexes induce periodontal tissue regeneration. J Dent Res 96: 984-991.
  • Tavakolinejad S, Alamdari DH, Khajehahmadi S, Khordad E, Ebrahimzadeh-Bideskan A (2014). Cleft palate reconstruction by platelet-rich-plasma and stem cell injection: histological evidences. J Cell Anim Biol 8: 114-123.
  • Tsumanuma Y, Iwata T, Washio K, Yoshida T, Yamada A, Takagi R, Ohno T, Lin K, Yamato M, Ishikawa I et al (2011). Comparison of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model. Biomaterials 32: 5819-5825.
  • Vanarsdall R (1994). Periodontal/orthodontic interrelationships. In: Vanarsdall R, Graber TM, editors. Orthodontics: Current Principles and Techniques. 1st ed. St Louis, MO, USA: Mosby, pp. 623-666.
  • Yang Y, Rossi FM, Putnins EE (2010). Periodontal regeneration using engineered bone marrow mesenchymal stromal cells. Biomaterials 31: 8574-8582.
APA gul amuk n, KURT G, KARTAL YANDIM M, adan a, Baran Y (2018). A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study. , 382 - 391. 10.3906/biy-1712-62
Chicago gul amuk nisa,KURT Gökmen,KARTAL YANDIM Melis,adan aysun,Baran Yusuf A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study. (2018): 382 - 391. 10.3906/biy-1712-62
MLA gul amuk nisa,KURT Gökmen,KARTAL YANDIM Melis,adan aysun,Baran Yusuf A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study. , 2018, ss.382 - 391. 10.3906/biy-1712-62
AMA gul amuk n,KURT G,KARTAL YANDIM M,adan a,Baran Y A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study. . 2018; 382 - 391. 10.3906/biy-1712-62
Vancouver gul amuk n,KURT G,KARTAL YANDIM M,adan a,Baran Y A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study. . 2018; 382 - 391. 10.3906/biy-1712-62
IEEE gul amuk n,KURT G,KARTAL YANDIM M,adan a,Baran Y "A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study." , ss.382 - 391, 2018. 10.3906/biy-1712-62
ISNAD gul amuk, nisa vd. "A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study". (2018), 382-391. https://doi.org/10.3906/biy-1712-62
APA gul amuk n, KURT G, KARTAL YANDIM M, adan a, Baran Y (2018). A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study. Turkish Journal of Biology, 42(5), 382 - 391. 10.3906/biy-1712-62
Chicago gul amuk nisa,KURT Gökmen,KARTAL YANDIM Melis,adan aysun,Baran Yusuf A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study. Turkish Journal of Biology 42, no.5 (2018): 382 - 391. 10.3906/biy-1712-62
MLA gul amuk nisa,KURT Gökmen,KARTAL YANDIM Melis,adan aysun,Baran Yusuf A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study. Turkish Journal of Biology, vol.42, no.5, 2018, ss.382 - 391. 10.3906/biy-1712-62
AMA gul amuk n,KURT G,KARTAL YANDIM M,adan a,Baran Y A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study. Turkish Journal of Biology. 2018; 42(5): 382 - 391. 10.3906/biy-1712-62
Vancouver gul amuk n,KURT G,KARTAL YANDIM M,adan a,Baran Y A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study. Turkish Journal of Biology. 2018; 42(5): 382 - 391. 10.3906/biy-1712-62
IEEE gul amuk n,KURT G,KARTAL YANDIM M,adan a,Baran Y "A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study." Turkish Journal of Biology, 42, ss.382 - 391, 2018. 10.3906/biy-1712-62
ISNAD gul amuk, nisa vd. "A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study". Turkish Journal of Biology 42/5 (2018), 382-391. https://doi.org/10.3906/biy-1712-62
Sayfa Oluşturulma Tarihi
23-04-2024 12:35

İLETİŞİM

TÜBİTAK ULAKBİM TR Dizin

Yüzüncüyıl, İşçi Blokları Mahallesi

Muhsin Yazıcıoğlu Caddesi No:51/C

06530 Çankaya / ANKARA +90 (312) 298 92 00

trdizin@tubitak.gov.tr

TÜBİTAK ULAKBİM Ulusal Akademik Ağ ve Bilgi Merkezi Cahit Arf Bilgi Merkezi © Tüm Hakları Saklıdır. Gizlilik Politikası Kullanım Şartları