TY  - JOUR
TI  - A minimally invasive transfer method of mesenchymal stem cells to the intact periodontal ligament of rat teeth: a preliminary study
AB  - The aim of this study was to introduce a minimally invasive procedure for mesenchymal stem cell (MSC) transfer into theintact periodontal ligament (PDL) of the molar teeth in rats. Ten 12-week-old Wistar albino rats were used for this preliminary study.MSCs were obtained from bones of two animals and were labeled with green fluorescent protein (GFP). Four animals were randomlyselected for MSC injection, while 4 animals served as a control group. Samples were prepared for histological analysis, Cox-2 mRNAexpression polymerase chain reaction analysis, and fluorescent microscopy evaluation. The number of total cells, number of osteoclasticcells, and Cox-2 mRNA expression levels of the periodontal tissue of teeth were calculated. The number of total cells was increased withMSC injections in PDL significantly (P < 0.001). The number of osteoclastic cells and Cox-2 mRNA expression were found to be similarfor the two groups. GFP-labeled MSCs were observed with an expected luminescence on the smear samples of the PDL with transferredMSCs. The results of this preliminary study demonstrate successful evidence of transferring MSCs to intact PDL in a nonsurgical wayand offer a minimally invasive procedure for transfer of MSCs to periodontal tissues.
AU  - adan, aysun
AU  - Baran, Yusuf
AU  - gul amuk, nisa
AU  - KARTAL YANDIM, Melis
AU  - KURT, Gökmen
DO  - 10.3906/biy-1712-62
PY  - 2018
JO  - Turkish Journal of Biology
VL  - 42
IS  - 5
SN  - 1300-0152
SP  - 382
EP  - 391
DB  - TRDizin
UR  - http://search/yayin/detay/349195
ER  -