TY  - JOUR
TI  - In vitro anticancer effect of tricyclic antidepressant nortriptyline on multiple myeloma
AB  - Drug repurposing has been proved to be an effective strategy to meet the urgent need for novel anticancer agents for multiplemyeloma (MM) treatment. In this work, we aimed to investigate the anticancer effect and mechanism of tricyclic antidepressantnortriptyline (NTP) on the U266 MM cell line. The in vitro inhibitory effect of NTP at various doses and time points was studied.The combination potential of cisplatin-NTP was also investigated. Cell cycle analysis and three flow cytometric apoptosis assays wereperformed. NTP showed dose- and time-dependent inhibitory effects on the U266 MM cell line. NTP had greater inhibitory effectthan cisplatin ($IC_{50}$ 26 µM vs. 40 µM). The cisplatin-NTP combination is antagonistic. In addition to G2/M phase cell cycle arrest,NTP induced apoptosis as indicated by mitochondrial membrane potential and caspase-3 and annexin V assays. NTP has inhibitoryand apoptotic effects on U266 MM cells. The cisplatin-NTP combination indicated strong antagonism, which may have significantclinical relevance since antidepressants are commonly employed in adjuvant therapy for cancer patients. Based on these findings, thetherapeutic potential of NTP for MM treatment should be investigated with in-depth mechanistic studies and in vivo experiments.
AU  - ÖZEN, Can
AU  - BİBER, Ayşenur
AU  - DURUSU, İpek Z.
DO  - 10.3906/biy-1802-11
PY  - 2018
JO  - Turkish Journal of Biology
VL  - 42
IS  - 5
SN  - 1300-0152
SP  - 414
EP  - 421
DB  - TRDizin
UR  - http://search/yayin/detay/349205
ER  -