TY  - JOUR
TI  - Calcium Mobilization and Inhibition of Akt Reduced the Binding of PEO-1 Cells to Fibronectin
AB  - Objectives: To investigate the effects of intracellular calcium (Ca2+) mobilization, β-catenin and Akt signal pathways after the binding of metastaticovarian cells to fibronectin.Materials and Methods: The expression levels of α4β1 and αvβ6 integrin were determined using α4, β1, αv, and β6 antibodies using flow cytometryon PEO-1 cells. The effect of [Ca2+]i on cell adhesion capacity was investigated using RTCA after stimulating PEO-1 cells using thapsigargin andtunicamycin. The binding rate of PEO-1 cells to fibronectin was also investigated in the presence of either different concentrations of cardamonin,which inhibits the accumulation of β-catenin, or different concentrations of FPA 124, which is a specific inhibitor for the PKB/Akt signal pathway,using RTCA.Results: RTCA analysis results showed that increasing [Ca2+]i through leakage of the calcium pool was strongly effective on PEO-1 cell binding tofibronectin. Extracellular calcium influx also reduced the binding of PEO-1 cells. Cell binding to fibronectin was also inhibited with a ratio of 64%in the presence of 100 µM cardamonin compared with untreated control cells. Finally, it was found that PKB/Akt inhibition with 15 µM FPA 124decreased the binding of PEO-1 cells to fibronectin with a ratio of 88% compared with untreated control cells.Conclusion: PEO-1 cell binding to fibronectin via integrins could be related to intracellular Ca2+ mobilization and Akt signaling.
AU  - İNCESU, Zerrin
AU  - KILIÇASLAN, Seda Mehtap SARI
AU  - AYRIM, Aysun
AU  - APAYDIN, ELİF
DO  - 10.4274/tjps.35220
PY  - 2018
JO  - Turkish Journal of Pharmaceutical Sciences
VL  - 15
IS  - 1
SN  - 1304-530X
SP  - 50
EP  - 56
DB  - TRDizin
UR  - http://search/yayin/detay/353582
ER  -