The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma

Objective: Angiotensin II promotes growth and angiogenesis viatype 1 receptors (AGTR1) in certain tumors. In this study, we examinethe bone marrow AGTR1 expression in multiple myeloma (MM) andits relationship with the regulation of angiogenesis and prognosticfactors.Materials and Methods: Bone marrow AGTR1 mRNA levels of 39 MMpatients and 15 healthy controls were analyzed with quantitative RTPCR. Immunohistochemical staining of the tissue vascular endothelialgrowth factor (VEGF), CD34, and factor VIIIrAg (fVIIIrAg) was used toassess bone marrow angiogenesis.Results: Bone marrow samples of the patients showed increasedVEGF, fVIIIrAg, and CD34 staining and higher AGTR1 expression levelswhen compared to controls. Patients with severe-diffuse bone marrowinfiltration showed higher bone marrow VEGF, fVIIIrAg, CD34, andAGTR1 mRNA levels when compared to other patients.Conclusion: AGTR1 expression was found positively correlated withplasma β2-microglobulin level and patients with increased AGTR1expression showed increased bone marrow CD34 levels.

Anahtar Kelime:

Multipl Myelomda Lokal Kemik İliği Renin-Anjiyotensin Sisteminin Rolü

Öz:

Amaç: Anjiyotensin II, bazı tümörlerde tip 1 reseptörleri (AGTR1) yoluyla bir büyüme promotörü veya anjiyojenik faktör olarak görev yapar. Bu çalışmada, multipl myelomda (MM) kemik iliği AGTR1 ekspresyonunu ve bunun anjiyogenez ve prognostik faktörlerin düzenlenmesi ile ilişkisini incelemekteyiz. Gereç ve Yöntemler: Otuz dokuz MM hastası ve 15 sağlıklı kontrolde, kemik iliği AGTR1 mRNA düzeyleri kantitatif RT-PCR ile analiz edildi. Kemik iliği anjiogenezi, doku vasküler endotel büyüme faktörü (VEGF), CD34 ve faktör VIIIrAg’nın (fVIIIrAg) immünohistokimyasal ölçümü ile değerlendirildi. Bulgular: Hastalar kontrollerle karşılaştırıldığında daha yüksek kemik iliği VEGF, fVIIIrAg, CD34 ve AGTR1 ekspresyon seviyelerine sahipti ve şiddetli yaygın kemik iliği infiltrasyonu olan hastalar diğer hastalara göre daha yüksek kemik iliği VEGF, fVIIIrAg, CD34 ve AGTR1 mRNA seviyeleri gösterdi. Sonuç: AGTR1 ekspresyonu, plazma β2-mikroglobulin seviyesi ile korele bulundu ve artan AGTR1 ekspresyonu olan hastalarda kemik iliği CD34 seviyelerinde artış görüldü.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Saka B, Aydın Sayitoglu M, İstemihan Z, KARAN M, erten s, DOĞAN Ö, ÖZBEK U, Genc S, Tascioglu C, Erdem S (2019). The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma. , 178 - 185. 10.4274/tjh.galenos.2019.2018.0420
Chicago	Saka Bulent,Aydın Sayitoglu Muge,İstemihan Zülal,KARAN MEHMET,erten sebile nilgün,DOĞAN Öner,ÖZBEK Uğur,Genc Sema,Tascioglu Cemil,Erdem Simge The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma. (2019): 178 - 185. 10.4274/tjh.galenos.2019.2018.0420
MLA	Saka Bulent,Aydın Sayitoglu Muge,İstemihan Zülal,KARAN MEHMET,erten sebile nilgün,DOĞAN Öner,ÖZBEK Uğur,Genc Sema,Tascioglu Cemil,Erdem Simge The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma. , 2019, ss.178 - 185. 10.4274/tjh.galenos.2019.2018.0420
AMA	Saka B,Aydın Sayitoglu M,İstemihan Z,KARAN M,erten s,DOĞAN Ö,ÖZBEK U,Genc S,Tascioglu C,Erdem S The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma. . 2019; 178 - 185. 10.4274/tjh.galenos.2019.2018.0420
Vancouver	Saka B,Aydın Sayitoglu M,İstemihan Z,KARAN M,erten s,DOĞAN Ö,ÖZBEK U,Genc S,Tascioglu C,Erdem S The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma. . 2019; 178 - 185. 10.4274/tjh.galenos.2019.2018.0420
IEEE	Saka B,Aydın Sayitoglu M,İstemihan Z,KARAN M,erten s,DOĞAN Ö,ÖZBEK U,Genc S,Tascioglu C,Erdem S "The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma." , ss.178 - 185, 2019. 10.4274/tjh.galenos.2019.2018.0420
ISNAD	Saka, Bulent vd. "The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma". (2019), 178-185. https://doi.org/10.4274/tjh.galenos.2019.2018.0420

APA	Saka B, Aydın Sayitoglu M, İstemihan Z, KARAN M, erten s, DOĞAN Ö, ÖZBEK U, Genc S, Tascioglu C, Erdem S (2019). The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma. Turkish Journal of Hematology, 36(3), 178 - 185. 10.4274/tjh.galenos.2019.2018.0420
Chicago	Saka Bulent,Aydın Sayitoglu Muge,İstemihan Zülal,KARAN MEHMET,erten sebile nilgün,DOĞAN Öner,ÖZBEK Uğur,Genc Sema,Tascioglu Cemil,Erdem Simge The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma. Turkish Journal of Hematology 36, no.3 (2019): 178 - 185. 10.4274/tjh.galenos.2019.2018.0420
MLA	Saka Bulent,Aydın Sayitoglu Muge,İstemihan Zülal,KARAN MEHMET,erten sebile nilgün,DOĞAN Öner,ÖZBEK Uğur,Genc Sema,Tascioglu Cemil,Erdem Simge The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma. Turkish Journal of Hematology, vol.36, no.3, 2019, ss.178 - 185. 10.4274/tjh.galenos.2019.2018.0420
AMA	Saka B,Aydın Sayitoglu M,İstemihan Z,KARAN M,erten s,DOĞAN Ö,ÖZBEK U,Genc S,Tascioglu C,Erdem S The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma. Turkish Journal of Hematology. 2019; 36(3): 178 - 185. 10.4274/tjh.galenos.2019.2018.0420
Vancouver	Saka B,Aydın Sayitoglu M,İstemihan Z,KARAN M,erten s,DOĞAN Ö,ÖZBEK U,Genc S,Tascioglu C,Erdem S The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma. Turkish Journal of Hematology. 2019; 36(3): 178 - 185. 10.4274/tjh.galenos.2019.2018.0420
IEEE	Saka B,Aydın Sayitoglu M,İstemihan Z,KARAN M,erten s,DOĞAN Ö,ÖZBEK U,Genc S,Tascioglu C,Erdem S "The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma." Turkish Journal of Hematology, 36, ss.178 - 185, 2019. 10.4274/tjh.galenos.2019.2018.0420
ISNAD	Saka, Bulent vd. "The Role of the Local Bone Marrow Renin-Angiotensin System in Multiple Myeloma". Turkish Journal of Hematology 36/3 (2019), 178-185. https://doi.org/10.4274/tjh.galenos.2019.2018.0420