TY - JOUR TI - In vitro Multiple Shoot Regeneration from Petunia hybrida AB - An efficient plant regeneration protocol was developed from in vitro germinated seeds of Petuniahybrida an ornamentally important plant in the family Solanaceae. Shoot tip and node explants ofPetunia hybrida were cultured on MS basal medium supplemented with different concentrations andcombinations of Benzyl amino purine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-butyricacid (IBA) and Gibberellic acid (GA3). The highest shoot length was obtained from MS mediumsupplemented with 1 mg/l BAP + 1 mg/l NAA. The highest shoot number (3 shoots/explant) wereobtained from MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBA. The isolated shootswere transferred to MS basal medium supplemented with different concentrations of GA3 rangingfrom 0.05, 0.2, 0.5 and 1 mg/l for shoot elongation. The highest shoot length (5.75 cm) was recordedfrom the MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAP. Rooting of regeneratedshoots were achieved on MS medium supplemented with 0.1-1 mg/1 IBA and NAA. The regeneratedshoots with well developed roots were successfully acclimatized and established in pots containingsterilized peat moss and grown under laboratory conditions with 70% survival rates. AU - özdemir, Fethi Ahmet AU - turker, musa AU - Habas, Rebaz Resul DO - 10.24925/turjaf.v7i10.1554-1560.2570 PY - 2019 JO - Türk Tarım - Gıda Bilim ve Teknoloji dergisi VL - 7 IS - 10 SN - 2148-127X SP - 1554 EP - 1560 DB - TRDizin UR - http://search/yayin/detay/355057 ER -