Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species

GÖKBULUT, Alper; ALTUN, M. Levent; ŞABANOĞLU, Selman

doi:10.12991/jrp.2019.164

Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species

Selman ŞABANOĞLU, (Ankara Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Ankara, Türkiye)

Alper GÖKBULUT, (Ankara Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Ankara, Türkiye)

M. Levent ALTUN (Ankara Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Ankara, Türkiye)

Journal of research in pharmacy (online)

3 0

Yıl: 2019 Cilt: 23 Sayı: 3 Sayfa Aralığı: 567 - 576 Metin Dili: İngilizce DOI: 10.12991/jrp.2019.164 İndeks Tarihi: 17-09-2020

Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species

Öz:

Medicinal plants used in traditional medicine have been increasingly noticed within recent years in pharmaceutical, cosmetic and nutraceutical industry. Herein, methanolic extracts of Achillea biebersteinii Afan., A. setacea Waldst. Et Kit. and A. wilhelmsii C. Koch were investigated for their total phenolic content and antioxidant activity with the main focus on phenolics. The total phenolic content varied widely in different parts of the three tested Achillea species, ranging from 113.2±1.9 to 178.4±9.5 mg GAE/g extract using Folin Ciocalteu method. DPPH and ABTS antioxidant activity tests were used to evaluate and compare the antioxidant activity of the species. Both the DPPH and ABTS assay results revealed that the radical scavenging activity of A. biebersteinii leaf extract is remarkably high with IC50 values of 0.377±0.011 and 0.016±0.002 mg/mL, respectively. Furthermore, a newly validated RP-HPLC-DAD method was developed and used to determine the phenolic compound profile of the methanol extracts. Phenolic components, such as gallic acid, chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, rutin, quercetin, luteolin, apigenin and kaempferol were analyzed by HPLC-DAD. The significant antioxidant properties of the extracts could be attributed to the phenolics of the Achillea species.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

[1] Si XT, Zhang ML, Shi QW, Kiyota H. Chemical constituents of the plants in the genus Achillea. Chem Biodivers. 2006; 3: 1163–1180. [CrossRef]
[2] Fathi H, Lashtoo AB, Ebrahimzadeh MA. Antioxidant activity and phenolic contents of Achillea wilhemsii. Pharmacologyonline. 2011; 2: 942-949.
[3] Baytop T. Therapy with medicinal plants in Turkey. Sanal Press, Istanbul, 1984, pp.176-177.
[4] Karaalp C, Yurtman AN, Karabay YNU. Evaluation of antimicrobial properties of Achillea L. flower head extracts. Pharm Biol. 2009; 47: 86-91. [CrossRef]
[5] Shahidi F. Antioxidants in food and food antioxidants. Nahrung. 2000; 44: 158–163. [CrossRef]
[6] Antolovich M, Prenzler PD, Patsalides E, McDonald S, Robards K. Methods for testing antioxidant activity. Analyst. 2002; 127: 183-198. [CrossRef]
[7] Konyalıoğlu S, Karamenderes C. The protective effects of Achillea L. species native in Turkey against H2O2-induced oxidative damage in human erythrocytes and leucocytes. J Ethnopharmacol. 2005; 102: 221–227. [CrossRef]
[8] Benetis R, Radusiene J, Jakstas V, Janulis V, Poudziunuene G, Milasius A. Quantitative HPLC determination of phenolic compounds in yarrow. Pharm Chem J. 2008; 42: 51-54. [CrossRef]
[9] Shahidi F, Wanasundara P. Phenolic antioxidants. Crit Rev Food Sci Nutr. 1992; 32: 67–103. [CrossRef]
[10] de-Beer D, Joubert E, Gerlderblom WCA, Manley M. Phenolic compounds: A review of their possible role as in vivo antioxidants of wine. S Afr J Enol Vitic. 2002; 23: 48-61. [CrossRef]
[11] Bobis O, Dezmirean DS, Tomos L, Chirila F, Marghitas LA. Influence of phytochemical profile on antibacterial activity of different medicinal plants against gram-positive and gram-negative bacteria. Applied Biochem Micro. 2015; 51: 113-118. [CrossRef]
[12] Trumbeckaite S, Benetis R, Bumblauskiene L, Burdulis D, Janulis V, Toleikis A, Viskelis P, Jakstas V. Achillea millefolium L. s.l. herb extract: Antioxidant activity and effect on the rat heart mitochondrial functions. Food Chem. 2011; 127: 1540-1548. [CrossRef]
[13] Raudonis R, Jakstas V, Burdulis D, Benetis R, Janulis V. Investigation of contribution of individual constituents to antioxidant activity in herbal drugs using post column HPLC method. Medicina (Kaunas). 2009; 45: 382-394. [CrossRef]
[14] Vitalini S, Beretta G, Iriti M, Orsenigo S, Basilico N, Dallacqua S, Lorizii M, Fico G. Phenolic compounds from Achillea millefolium L. and their bioactivity. Acta Biochim Pol. 2011; 58(2): 203-209.
[15] Bashi DS, Fazly BBS, Sahebkar A, Karimkhani MM, Ahmadi A. Investigation of optimal extraction, antioxidant, and antimicrobial activities of Achillea biebersteinii and A. wilhelmsii. Pharm Biol. 2012; 50: 1168-1176. [CrossRef]
[16] Baris D, Kizil M, Aytekin C, Kizil G, Yavuz M, Ceken B, Ertekin AS. In vitro antimicrobial and antioxidant activity of ethanol extract of three Hypericum and three Achillea species from Turkey. Int J Food Prop. 2011; 14: 339-355. [CrossRef]
[17] Ashgar M, Rasool N, Riaz M, Abass M, Mustafa AU, Adeel HM, Zubair M, Tareen RB, Rana UA, Ansari AR. Antioxidant and antimicrobial study of various parts of Achillea wilhelmsii (a medicinal plant). Oxid Commun. 2014; 37: 543-554.
[18] Gharibi S, Tabatabaei BES, Saeidi G, Goli SAH, Talebi M. Total phenolic content and antioxidant activity of three Iranian endemic Achillea species. Ind Crop Prod. 2013; 50: 154-158. [CrossRef]
[19] Benedec D, Vlase L, Oniga I, Mot AC, Damian G, Hanganu D, Duma M, Silaghi-Dumitrescu R. Polyphenolic composition, antioxidant and antibacterial activities for two Romanian subspecies of Achillea distans Waldst. et Kit. ex Willd. Molecules. 2013; 18: 8725-8739. [CrossRef]
[20] Marchart E, Kopp BC. Capillary electrophoretic separation and quantification of flavone-O and C-glycosides in Achillea setacea W. et K. J Chromatogr B. 2003; 792: 363-368. [CrossRef]
[21] Gökbulut A. Validated RP-HPLC method for quantification of phenolic compounds in methanol extracts of aerial parts and roots of Thymus sipyleus and evaluation of antioxidant potential. Trop J Pharm Res. 2015; 14: 1871-1877. [CrossRef]
[22] Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Method Enzymol. 1999; 299: 152–78. [CrossRef]
[23] Barros L, Baptista P, Ferreira ICFR. Effect of Lactarius piperatus fruiting body maturity stage on antioxidant activity measured by several biochemical assays. Food Chem Toxicol. 2007; 45: 1731-1737. [CrossRef]
[24] Re R, Pellegrin N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med. 1999; 26: 1231-1237. [CrossRef]
[25] IFPMA, “ICH validation of analytical procedures: text and methodology Q2 (R1),” Proceedings of the International Conference on Harmonization, IFPMA; 2005, Geneva, Switzerland.

APA	ŞABANOĞLU S, GÖKBULUT A, ALTUN M (2019). Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. , 567 - 576. 10.12991/jrp.2019.164
Chicago	ŞABANOĞLU Selman,GÖKBULUT Alper,ALTUN M. Levent Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. (2019): 567 - 576. 10.12991/jrp.2019.164
MLA	ŞABANOĞLU Selman,GÖKBULUT Alper,ALTUN M. Levent Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. , 2019, ss.567 - 576. 10.12991/jrp.2019.164
AMA	ŞABANOĞLU S,GÖKBULUT A,ALTUN M Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. . 2019; 567 - 576. 10.12991/jrp.2019.164
Vancouver	ŞABANOĞLU S,GÖKBULUT A,ALTUN M Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. . 2019; 567 - 576. 10.12991/jrp.2019.164
IEEE	ŞABANOĞLU S,GÖKBULUT A,ALTUN M "Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species." , ss.567 - 576, 2019. 10.12991/jrp.2019.164
ISNAD	ŞABANOĞLU, Selman vd. "Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species". (2019), 567-576. https://doi.org/10.12991/jrp.2019.164

APA	ŞABANOĞLU S, GÖKBULUT A, ALTUN M (2019). Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. Journal of research in pharmacy (online), 23(3), 567 - 576. 10.12991/jrp.2019.164
Chicago	ŞABANOĞLU Selman,GÖKBULUT Alper,ALTUN M. Levent Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. Journal of research in pharmacy (online) 23, no.3 (2019): 567 - 576. 10.12991/jrp.2019.164
MLA	ŞABANOĞLU Selman,GÖKBULUT Alper,ALTUN M. Levent Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. Journal of research in pharmacy (online), vol.23, no.3, 2019, ss.567 - 576. 10.12991/jrp.2019.164
AMA	ŞABANOĞLU S,GÖKBULUT A,ALTUN M Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. Journal of research in pharmacy (online). 2019; 23(3): 567 - 576. 10.12991/jrp.2019.164
Vancouver	ŞABANOĞLU S,GÖKBULUT A,ALTUN M Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species. Journal of research in pharmacy (online). 2019; 23(3): 567 - 576. 10.12991/jrp.2019.164
IEEE	ŞABANOĞLU S,GÖKBULUT A,ALTUN M "Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species." Journal of research in pharmacy (online), 23, ss.567 - 576, 2019. 10.12991/jrp.2019.164
ISNAD	ŞABANOĞLU, Selman vd. "Characterization of phenolic compounds, total phenolic content and antioxidant activity of three Achillea species". Journal of research in pharmacy (online) 23/3 (2019), 567-576. https://doi.org/10.12991/jrp.2019.164