Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour
Yıl: 2020 Cilt: 67 Sayı: 2 Sayfa Aralığı: 121 - 125 Metin Dili: İngilizce DOI: 10.33988/auvfd.492765 İndeks Tarihi: 06-10-2020
Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour
Öz: Canine transmissible venereal tumour (TVT) is a contagious cancer and sexually transmitted one dog to another by allogenic transfer of living cancer cells. The aim of this study is to examine the relationship between antioxidative metabolism and venereal tumour at Kangal Dogs and to show the size of oxidative damage it causes through 8-hydroxy-2’deoxyguanosine (8-OHdG). The materials of this study included the blood sera of 15 Kangal Dogs with TVT, and as a control group of 15 healthy Kangal Dogs. The sera levels of 8-OHdG were determined by competitive enzyme linked immunosorbent assay (ELISA) method. The level of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) which are antioxidative enzymes were investigated by spectrophotometric methods as described. Serum 8-OHdG and MDA levels of dogs with TVT were found to be significantly increased compared to the control group (P<0.001). The levels of GPx were statistically different (P<0.05). In the light of the findings, which are obtained in this study, the transmissible venereal tumour, which is an infectious and treatable type of cancer, can lead to new studies that may be caused by oxidant-antioxidant metabolism.
Anahtar Kelime: Venereal tümörlü Kangal köpeklerinde 8-hidroksi-2’deoksiguanozin, malondialdehit düzeyleri ile antioksidan enzim aktivitelerinin belirlenmesi
Öz: Transmissible (bulaşıcı) venereal tümör (TVT), canlı kanser hücrelerinin allojenik transferiyle bir köpekten diğer bir köpeğe çiftleşme ile bulaşan bir kanser türüdür. Bu çalışmada, Kangal köpeklerinde önemli bir bulaşıcı kanser türü olan venereal tümör ile antioksidatif metabolizmanın arasındaki ilişkinin incelenmesi ve oksidatif hasarın boyutunun 8 hidroksi-2’deoksiguanozin (8-OHdG) düzeyleri üzerinden gösterilmesi amaçlandı. Bu amaçla, 15 adet TVT’li, kontrol grubu olarak 15 adet sağlıklı Kangal köpeklerinden alınan toplam 30 adet kan serumu çalışmanın materyalini oluşturdu. Bu hayvanlara ait kan serumlarında 8-OHdG kompetitif enzim bağımlı immunosorbent analiz (ELISA) yöntemi ile belirlendi. Malondialdehit (MDA), antioksidan enzimler olan süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx) ve katalaz (CAT) seviyeleri ise spektrofotometrik yöntemlerle analiz edildi. TVT teşhisli köpeklerin serum 8-OHdG ve MDA düzeyleri kontrol grubundaki köpeklere göre istatiksel olarak anlamlı derecede artış gösterdiği tespit edildi (P<0,001). Antioksidan enzim olan GPx düzeylerinde istatiksel farklılık tespit edilmiştir (P<0,05). Bu çalışmada elde edilen sonuçlar neticesinde bulaşıcı ve tedavi edilebilir bir kanser türü olma niteliğindeki köpek bulaşıcı venereal tümörün oksidan antioksidan metabolizma kaynaklı yapılacak yeni çalışmalara yön gösterebilecek niteliktedir
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Aebi H (1984): Catalase in vitro. Methods Enzymol, 105, 121-6.
- 2. Aydın İ, Bülbül A, Avcı GE, et al (2009): Serum oxidative status and adenosine deaminase activity in dogs with transmissible venereal tumour. Bull Vet Inst Pulawy, 53, 771-774.
- 3. Baştan A, Baki Acar D, Cengiz M (2008): Uterine and ovarian metastasis of transmissible venereal tumour in a bitch. Turk J Vet Anim Sci, 32, 65-66.
- 4. Dinçer Y, Akçay T, Kural AR, et al (2011): Evaluation of 8-hydroxy-2'-deoxyguanosine concentration and antioxidant enzyme activities in bladder cancer patients. Turkiye Klinikleri J Med Sci, 31, 553-558.
- 5. Durak I, Yurtaslanı Z, Canbolat O, et al (1993): A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clin Chim Acta, 214,103-104.
- 6. Ercan N, Fidancı UR (2012): Piyodermalı köpeklerde 8- hidroksi-2’-deoksiguanozin (8-OHdG) düzeyleri. Ankara Üniv Vet Fak Derg, 59, 163-168.
- 7. Fraga C, Shigenaga M, Park J, et al (1990): Oxidative damage to DNA during aging: 8-Hydroxy-2’- deoxyguanosine in rat organ DNA urine. Proc Natl Acad Sci, 87, 4533-4537.
- 8. Gencer M, Ceyla E, Aksoy A, et al (2005): Oksidatif stres benign ve malign akciğer hastalıklarının ayırıcı tanısında belirteç olabilir mi? Akciğer Arşivi, 6, 89-92.
- 9. Haliwell B (2007): Oxidative stress and cancer: have we moved forward? Biochem J, 401, 1-11.
- 10. Hattori Y, Nishigori C, Tanaka T, et al (1996): 8- hydroxy-2’-deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure. J Invest Dermatol, 107, 733-737.
- 11. Janero DR (1990): Malondialdehyde and thiobarbituric acid-reactivy as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med, 9, 515-540.
- 12. Jayasri K, Padmaja K, Saibaba M (2016): Altered oxidative stress and carbohydrate metabolism in canine mammary tumors. Veterinary world, 9, 1489.
- 13. Kumaraguruparan R, Balachandran C, Manohar BM, et al (2005): Altered oxidant-antioxidant profile in canine mammary tumours, Vet Res Commun, 29, 287-96.
- 14. Lin H, Jenner A, Ong C, et al (2004): A high-throughput and sensitive methodology for the quantification of urinary 8-hydroxy-2’-deoxyguanosine: measurement with gas chromatography-mass spectrometry after single solidphase extraction. Biochem J, 380, 541-548.
- 15. Macotpet A, Suksawat F, Sukon P, et al (2013): Oxidative stress in cancer-bearing dogs assessed by measuring serum malondialdehyde. BMC Vet Res, 9, 101.
- 16. Martinis B, Bianchi M (2002): Methodology for urinary 8-hydroxy-2’-deoxyguanosine analysis by HPLC with electrochemical detection. Pharmacol Res, 46, 129-131.
- 17. Matsufuji H, Ochi H, Shibamoto T (2006): Formation and inhibition of genotoxic malonaldehyde from DNA oxidation controlled with EDTA. Food Chem Toxicol, 44, 236-241.
- 18. Nak D, Mısırlıoğlu D, Nak Y, et al (2004): Bir köpekte meme metastazlı transmissible venereal tümör olgusu. Vet Bil Derg, 20, 99-102.
- 19. Paglia DE, WN Valentine (1967): Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 70, 158-169.
- 20. Pilger A, Ivancsits S, Germadnik D, et al (2002): Urinaryexcretion of 8-hydroxy-2’-deoxyguanosine measured by high-performance liquid chromatography with electrochemical detection. J Chromatogr B, 778, 393-401.
- 21. SPSS (2014): IBM SPSS Statistics for Windows, version22.00. SPSS Inc., Chicago, IL.
- 22. Strakova A, Murchison EP (2014): The changing global distribution and prevalence of canine transmissible venereal tumour. BMC Vet Res, 10, 168.
- 23. Sun Y, Oberley LW, Li Y (1988): A simple method for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500.
- 24. Tanja P, Alenka NS, Butinar J, et al (2008): Antioxidant status in canine cancer patients. Acta Vet Beograd, 58, 275-286.
- 25. Tüközkan N, Erdamar H, Seven I (2006): Measurement of total malondialdehyde in plasma and tissues by highperformance liquid chromatography and thiobarbituric acid assay. Fırat Tıp Derg, 11, 88-92.
- 26. Urso ML, Clarkson PM (2003): Oxidative stress, exercise and antioxidant supplementation. Toxicology, 189, 41-54.
- 27. Welsh JS (2011). Contagious Cancer. The Oncologist, 16,1-4.
- 28. Winter JL, Barber LG., Freeman L, et al (2009): Antioxidant status and biomarkers of oxidative stress in dogs with Lymphoma. J Vet Intern Med, 23, 311-316.
- 29. Wiseman H, Kaur H, Halliwell B (1995): DNA damage and cancer: measurement and mechanism. Cancer Letters, 93, 113-120.
- 30. Wu LL, Chiou CC, Chang PY, et al (2004): Urinary 8- OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics Clin Chim Acta, 339, 1-9.
- 31. Zhang F, Stott WT, Clark AJ, et al (2007): Quantitation of 8-hydroxydeoxyguanosine in DNA by liquid chromatography/positive atmospheric pressure photoionization tandem mass spectrometry. Rapid Commun Mass Spectrom, 21, 3949-3955.
| APA | Ercan N, YÜKSEL M, KOÇKAYA M (2020). Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. , 121 - 125. 10.33988/auvfd.492765 |
| Chicago | Ercan Nazli,YÜKSEL MURAT,KOÇKAYA Mustafa Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. (2020): 121 - 125. 10.33988/auvfd.492765 |
| MLA | Ercan Nazli,YÜKSEL MURAT,KOÇKAYA Mustafa Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. , 2020, ss.121 - 125. 10.33988/auvfd.492765 |
| AMA | Ercan N,YÜKSEL M,KOÇKAYA M Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. . 2020; 121 - 125. 10.33988/auvfd.492765 |
| Vancouver | Ercan N,YÜKSEL M,KOÇKAYA M Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. . 2020; 121 - 125. 10.33988/auvfd.492765 |
| IEEE | Ercan N,YÜKSEL M,KOÇKAYA M "Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour." , ss.121 - 125, 2020. 10.33988/auvfd.492765 |
| ISNAD | Ercan, Nazli vd. "Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour". (2020), 121-125. https://doi.org/10.33988/auvfd.492765 |
| APA | Ercan N, YÜKSEL M, KOÇKAYA M (2020). Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 67(2), 121 - 125. 10.33988/auvfd.492765 |
| Chicago | Ercan Nazli,YÜKSEL MURAT,KOÇKAYA Mustafa Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67, no.2 (2020): 121 - 125. 10.33988/auvfd.492765 |
| MLA | Ercan Nazli,YÜKSEL MURAT,KOÇKAYA Mustafa Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.67, no.2, 2020, ss.121 - 125. 10.33988/auvfd.492765 |
| AMA | Ercan N,YÜKSEL M,KOÇKAYA M Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2020; 67(2): 121 - 125. 10.33988/auvfd.492765 |
| Vancouver | Ercan N,YÜKSEL M,KOÇKAYA M Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2020; 67(2): 121 - 125. 10.33988/auvfd.492765 |
| IEEE | Ercan N,YÜKSEL M,KOÇKAYA M "Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 67, ss.121 - 125, 2020. 10.33988/auvfd.492765 |
| ISNAD | Ercan, Nazli vd. "Determination of 8-hydroxy-2’deoxyguanosine, malondialdehyde levels and antioxidant enzyme activities in Kangal dogs with venereal tumour". Ankara Üniversitesi Veteriner Fakültesi Dergisi 67/2 (2020), 121-125. https://doi.org/10.33988/auvfd.492765 |
