Yıl: 2019 Cilt: 20 Sayı: 2 Sayfa Aralığı: 91 - 97 Metin Dili: Türkçe İndeks Tarihi: 08-10-2020

TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR

Öz:
AMAÇ: Leptin hormonu, iştah ve vücut metabolizması-nın düzenlenmesinde önemli görevler üstlenmekte vebaşlıca yağ dokusunda sentezlenmektedir. Leptinin yağdokuda sentezlendikten sonra koroid pleksus aracılığı ilebeyne taşındığı bilinmektedir. Bu çalışmanın amacı Tip 1diyabetes mellitus oluşturulan sıçanların beyninde leptinifadesinin ve miktarının araştırılmasıdır.GEREÇ VE YÖNTEM: Çalışmamızda toplam 14 adet yetiş-kin, erkek Wistar Albino sıçan 2 eşit gruba ayrıldı (n=7).Kontrol grubuna hiçbir uygulama yapılmadı. Diyabetesmellitus grubundaki hayvanlara ise tek doz (55 mg/kg)streptozotosin intraperitoneal olarak verildi ve kan glukozseviyesi >280 mg/dL ölçüldüğünde Tip 1diyabetes mellitus geliştiği kabul edildi. Deney sonunda alınan beyinörnekleri %10’luk formaldehit ile fikse edildikten sonrarutin doku takip işleminin ardından alınan kesitlere leptinimmünohistokimyasal boyaması uygulandı. Beyin dokularında western blot yöntemi ile leptin miktarına bakıldı.BULGULAR: Tüm gruplara ait beyin kesitlerinde sadecekoroid pleksusta leptin boyanması saptandı. Buna göre,leptin boyanmasının Tip 1 diyabetes mellitus geliştirilensıçanlarda azaldığı saptandı. Western blot ile Tip 1diyabetes mellitus grubunda leptin miktarının belirginolarak azaldığı saptandı.SONUÇ: Bu çalışmayla tokluk hissini oluşturmaküzere koroid pleksus üzerinden beyne geçen leptin hormonu ile diyabet arasında yakın bir ilişki olduğu ve bu hormonun diyabetle azaldığı gösterilmiştir. Bu çalışmadan yola çıkarak Tip 1 diyabet veleptin hormonu ilişkisi derinlemesine irdelenmelidir.
Anahtar Kelime:

LEPTIN QUANTITY DECREASES İN TYPE 1 DIABETES MELLITUS INDUCUED RATS BRAIN

Öz:
OBJECTIVE: Leptin hormone plays an important role in regulation of appetite and body metabolism and is mainly synthesized in fat tissue. It is known that leptin is transported to the brain via choroid plexus after synthesized in the fat tissue. The aim of this study is to investigate the amount and expression of leptin in the brain of rats with type 1 diabetes mellitus. MATERIAL AND METHODS: A total of 14 adult male Wistar Albino rats were divided into 2 equal groups (n = 7). No application was made to the control group. A single dose (55 mg/kg) of streptozotocin was administered intraperitoneally to the animals of the diabetes mellitus group and it was considered that type 1 diabetes mellitus developed when blood glucose level > 280 mg/dL. Brain samples taken at the end of the experiment were fixed with %10 formaldehyde and then subjected to routine tissue monitoring followed by leptin immunohistochemical staining. The amount of leptin was determined by western blotting in brain tissues. RESULTS: In all groups leptin staining was detected only in the choroid plexus of rat brains. According to this knowlodge, it was determined that leptin staining decreased in type 1 diabetes mellitus developed rats. The decreased leptin in the type 1 diabetes mellitus group was showed by western blot too. CONCLUSIONS: Type 1 diabetes reduces the expression and amount of leptin in the choroid plexus.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA SAHIN E, ÖZCAN Ö, BEKTUR E, BAYÇU C, DEMİR Ü, CAN Ö, ŞAHİNTÜRK V (2019). TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR. , 91 - 97.
Chicago SAHIN Erhan,ÖZCAN Öykü,BEKTUR Ezgi,BAYÇU Cengiz,DEMİR Ümide Özkay,CAN Özgür Devrim,ŞAHİNTÜRK Varol TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR. (2019): 91 - 97.
MLA SAHIN Erhan,ÖZCAN Öykü,BEKTUR Ezgi,BAYÇU Cengiz,DEMİR Ümide Özkay,CAN Özgür Devrim,ŞAHİNTÜRK Varol TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR. , 2019, ss.91 - 97.
AMA SAHIN E,ÖZCAN Ö,BEKTUR E,BAYÇU C,DEMİR Ü,CAN Ö,ŞAHİNTÜRK V TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR. . 2019; 91 - 97.
Vancouver SAHIN E,ÖZCAN Ö,BEKTUR E,BAYÇU C,DEMİR Ü,CAN Ö,ŞAHİNTÜRK V TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR. . 2019; 91 - 97.
IEEE SAHIN E,ÖZCAN Ö,BEKTUR E,BAYÇU C,DEMİR Ü,CAN Ö,ŞAHİNTÜRK V "TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR." , ss.91 - 97, 2019.
ISNAD SAHIN, Erhan vd. "TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR". (2019), 91-97.
APA SAHIN E, ÖZCAN Ö, BEKTUR E, BAYÇU C, DEMİR Ü, CAN Ö, ŞAHİNTÜRK V (2019). TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi, 20(2), 91 - 97.
Chicago SAHIN Erhan,ÖZCAN Öykü,BEKTUR Ezgi,BAYÇU Cengiz,DEMİR Ümide Özkay,CAN Özgür Devrim,ŞAHİNTÜRK Varol TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi 20, no.2 (2019): 91 - 97.
MLA SAHIN Erhan,ÖZCAN Öykü,BEKTUR Ezgi,BAYÇU Cengiz,DEMİR Ümide Özkay,CAN Özgür Devrim,ŞAHİNTÜRK Varol TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi, vol.20, no.2, 2019, ss.91 - 97.
AMA SAHIN E,ÖZCAN Ö,BEKTUR E,BAYÇU C,DEMİR Ü,CAN Ö,ŞAHİNTÜRK V TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi. 2019; 20(2): 91 - 97.
Vancouver SAHIN E,ÖZCAN Ö,BEKTUR E,BAYÇU C,DEMİR Ü,CAN Ö,ŞAHİNTÜRK V TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi. 2019; 20(2): 91 - 97.
IEEE SAHIN E,ÖZCAN Ö,BEKTUR E,BAYÇU C,DEMİR Ü,CAN Ö,ŞAHİNTÜRK V "TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR." Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi, 20, ss.91 - 97, 2019.
ISNAD SAHIN, Erhan vd. "TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR". Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi 20/2 (2019), 91-97.