Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits
Yıl: 2020 Cilt: 26 Sayı: 4 Sayfa Aralığı: 497 - 502 Metin Dili: İngilizce DOI: 10.14744/tjtes.2020.25260 İndeks Tarihi: 20-10-2020
Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits
Öz: BACKGROUND: Fibronectin (FN) is an indispensable part of the extracellular matrix. During regeneration or wound healing, theplasma form of FN is incorporated into the fibrin clots to form a temporary fibrin-FN matrix, and also locally synthesized cellular FNmigrates to the clot to regenerate the injured tissue. We aimed to examine wound tissue FN EIIIB and plasma FN EIIIB expressionlevels in an experimental wound healing model in rabbits.METHODS: Plasma and tissue EIIIB splice variant expressions were measured serially with RT-qPCR in a cutaneous wound modelof rabbits.RESULTS: Tissue FN expression increased as beginning on day 3 and continued to increase on days 6 and 9, reaching maximumexpression at day 12 before starting to decrease. On the contrary to the tissue levels, plasma FN levels gradually decreased until day15 when expression returned to the initial values.CONCLUSION: The findings of the current study support that tissue EIIIB expression level increases during wound healing; andplasma EIIIB expression level decreases minimal changed. This is in contrast to reports where plasma FN provisionally helps ECM formation.Therefore, our data show an essential role of EIIIB at the tissue level in accelerating the wound healing process. The RT-qPCRmethod in our experimental setup can provide more accurate and precise results compared to the antibody-based methods.
Anahtar Kelime: Tavşan yara modelinde RT-PCR yöntemi ile seri olarak ölçülen plazma ve doku fibronektin EIIIB splice değerlerinin ölçümleri
Öz: AMAÇ: Fibronektin (FN), hücre dışı matriksin vazgeçilmez bir parçasıdır. Rejenerasyon veya yara iyileşmesi sırasında FN’nin plazma formu, geçici bir fibrin-FN matrisi oluşturmak için fibrin pıhtılarına dahil olur ve ayrıca lokal olarak sentezlenen hücresel FN, yaralı dokuyu rejenere etmek için pıhtıya göç eder. Bu çalışmada tavşanlarda deneysel yara iyileşme modelinde yara dokusu EIIIB ve plazma EIIIB ekspresyon düzeylerini incelemeyi amaçladık. GEREÇ VE YÖNTEM: Plazma ve doku EIIIB ekspresyon düzeyleri, tavşanların kutanöz yara modelinde RT-qPCR ile seri olarak ölçüldü. BULGULAR: Doku FN ekspresyonu 3. günden başlayarak arttı, 6. ve 9. günlerde artmaya devam etti, azalmaya başlamadan önce 12. günde maksimum ekspresyona ulaştı. Doku seviyelerinin aksine, plazma FN seviyeleri ekspresyon başlangıç değerlerine döndüğünde 15. güne kadar kademeli olarak azaldı. TARTIŞMA: Sonuç olarak, mevcut çalışmanın bulguları, yara iyileşmesi sırasında doku EIIIB ekspresyon seviyesinin arttığını desteklemektedir; plazma EIIIB ekspresyon seviyesi minimum düzeyde azalır. Bu, plazma FN’nin geçici olarak ECM oluşumuna yardımcı olduğu raporların aksine değerlerdir. Bu nedenle verilerimiz, yara iyileşme sürecini hızlandırmada EIIIB’nin doku düzeyinde önemli bir rol oynadığını göstermektedir. Ayrıca, deneyde kullandığımız ölçme yöntemimiz olan RT-qPCR yöntemi, antikor bazlı yöntemlere kıyasla daha doğru ve kesin sonuçlar sağlayabilir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Maxson S, Lopez EA, Yoo D, Danilkovitch-Miagkova A, Leroux MA. Concise review: role of mesenchymal stem cells in wound repair. Stem Cells Transl Med 2012;1:142−9.
- 2. Singer AJ, Clark RA. Cutaneous wound healing. N Engl J Med 1999;341:738−46.
- 3. Lenselink EA. Role of fibronectin in normal wound healing. Int Wound J 2015;12:313−6.
- 4. Pankov R, Yamada KM. Fibronectin at a glance. J Cell Sci 2002;115:3861−3.
- 5. To WS, Midwood KS. Plasma and cellular fibronectin: distinct and independent functions during tissue repair. Fibrogenesis Tissue Repair 2011;4:21.
- 6. Peters JH, Loredo GA, Chen G, Maunder R, Hahn TJ, Willits NH, et al. Plasma levels of fibronectin bearing the alternatively spliced EIIIB segment are increased after major trauma. J Lab Clin Med 2003;141:401−10.
- 7. Peters JH, Trevithick JE, Johnson P, Hynes RO. Expression of the alternatively spliced EIIIB segment of fibronectin. Cell Adhes Commun 1995;3:67−89.
- 8. Paul W, Sharma CP. Chitosan and alginate wound dressings: A short review. Trends Biomater Artificial Organs 2004;18:18−23.
- 9. Al-Rawaf HA, Gabr SA, Alghadir AH. Circulating Hypoxia Responsive microRNAs (HRMs) and Wound Healing Potentials of Green Tea in Diabetic and Nondiabetic Rat Models. Evid Based Complement Alternat Med 2019;2019:9019253.
- 10. Clark RA. Fibrin and wound healing. Ann N Y Acad Sci 2001;936:355−67.
- 11. Takeo M, Lee W, Ito M. Wound healing and skin regeneration. Cold Spring Harb Perspect Med 2015;5:a023267.
- 12. Kallis PJ, Friedman AJ. Collagen Powder in Wound Healing. J Drugs Dermatol 2018;17:403−8.
- 13. Woodley DT. Distinct Fibroblasts in the Papillary and Reticular Dermis: Implications for Wound Healing. Dermatol Clin 2017;35:95−100.
- 14. Kim H, Kong WH, Seong KY, Sung DK, Jeong H, Kim JK, et al. Hyaluronate-Epidermal Growth Factor Conjugate for Skin Wound Healing and Regeneration. Biomacromolecules 2016;17:3694−705.
- 15. Sahin Inan ZD, Unver Saraydin S. Investigation of the wound healing effects of chitosan on FGFR3 and VEGF immunlocalization in experimentally diabetic rats. Int J Bio Materials Res 2013;1:1−8.
- 16. Johnson MB, Pang B, Gardner DJ, Niknam-Benia S, Soundarajan V, Bramos A, et al. Topical Fibronectin Improves Wound Healing of Irradiated Skin. Sci Rep 2017;7:3876.
- 17. Whittam AJ, Maan ZN, Duscher D, Wong VW, Barrera JA, Januszyk M, et al. Challenges and Opportunities in Drug Delivery for Wound Healing. Adv Wound Care (New Rochelle) 2016;5:79−88.
- 18. Hamed S, Ullmann Y, Egozi D, Daod E, Hellou E, Ashkar M, et al. Fibronectin potentiates topical erythropoietin-induced wound repair in diabetic mice. J Invest Dermatol 2011;131:1365−74.
- 19. Kwon AH, Qiu Z, Hiraon Y. Effect of plasma fibronectin on the incisional wound healing in rats. Surgery 2007;141:254−61.
- 20. Tomasini-Johansson BR, Zbyszynski PW, Toraason I, Peters DM, Kwon GS. PEGylated pUR4/FUD peptide inhibitor of fibronectin fibrillogenesis decreases fibrosis in murine Unilateral Ureteral Obstruction model of kidney disease. PLoS One 2018;13:e0205360.
- 21. Mao Y, Schwarzbauer JE. Fibronectin fibrillogenesis, a cell-mediated matrix assembly process. Matrix Biol 2005;24:389−99.
- 22. Magnusson MK, Mosher DF. Fibronectin: structure, assembly, and cardiovascular implications. Arterioscler Thromb Vasc Biol 1998;18:1363−70.
- 23. Kornblihtt AR, Umezawa K, Vibe-Pedersen K, Baralle FE. Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene. EMBO J 1985;4:1755−9.
- 24. Jarnagin WR, Rockey DC, Koteliansky VE, Wang SS, Bissell DM. Expression of variant fibronectins in wound healing: cellular source and biological activity of the EIIIA segment in rat hepatic fibrogenesis. J Cell Biol 1994;127:2037−48.
- 25. Takashima A, Billingham RE, Grinnell F. Activation of rabbit keratinocyte fibronectin receptor function in vivo during wound healing. J Invest Dermatol 1986;86:585−90.
- 26. Ongenae KC, Phillips TJ, Park HY. Level of fibronectin mRNA is markedly increased in human chronic wounds. Dermatol Surg 2000;26:447−51.
- 27. Sazuka M, Isemura M, Isemura S. Interaction between the carboxyl-terminal heparin-binding domain of fibronectin and (-)-epigallocatechin gallate. Biosci Biotechnol Biochem 1998;62:1031−2.
- 28. Dooley A, Shi-Wen X, Aden N, Tranah T, Desai N, Denton C, et al. Modulation of collagen type I, fibronectin and dermal fibroblast function and activity, in systemic sclerosis by the antioxidant epigallocatechin-3-gallate. Rheumatology (Oxford) 2010;49:2024−36.
- 29. Hocking DC, Brennan JR, Raeman CH. A Small Chimeric Fibronectin Fragment Accelerates Dermal Wound Repair in Diabetic Mice. Adv Wound Care (New Rochelle) 2016;5:495−506.
- 30. Morishige N, Uemura A, Morita Y, Nishida T. Promotion of Corneal Epithelial Wound Healing in Diabetic Rats by the Fibronectin-Derived Peptide PHSRN. Cornea 2017;36:1544−8.
- 31. Claudepierre P, Allanore Y, Belec L, Larget-Piet B, Zardi L, Chevalier X. Increased Ed-B fibronectin plasma levels in spondyloarthropathies: comparison with rheumatoid arthritis patients and a healthy population. Rheumatology (Oxford) 1999;38:1099−103.
- 32. Kwon AH, Qiu Z, Hirao Y. Topical application of plasma fibronectin in full-thickness skin wound healing in rats. Exp Biol Med (Maywood) 2007;232:935−41.
| APA | KURT OZKAYA N, ZEREYAK U, Acikalin Coskun K, TUTAR Y, yılmaz s (2020). Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits. , 497 - 502. 10.14744/tjtes.2020.25260 |
| Chicago | KURT OZKAYA Nese,ZEREYAK UMUT,Acikalin Coskun Kubra,TUTAR Yusuf,yılmaz sarper Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits. (2020): 497 - 502. 10.14744/tjtes.2020.25260 |
| MLA | KURT OZKAYA Nese,ZEREYAK UMUT,Acikalin Coskun Kubra,TUTAR Yusuf,yılmaz sarper Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits. , 2020, ss.497 - 502. 10.14744/tjtes.2020.25260 |
| AMA | KURT OZKAYA N,ZEREYAK U,Acikalin Coskun K,TUTAR Y,yılmaz s Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits. . 2020; 497 - 502. 10.14744/tjtes.2020.25260 |
| Vancouver | KURT OZKAYA N,ZEREYAK U,Acikalin Coskun K,TUTAR Y,yılmaz s Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits. . 2020; 497 - 502. 10.14744/tjtes.2020.25260 |
| IEEE | KURT OZKAYA N,ZEREYAK U,Acikalin Coskun K,TUTAR Y,yılmaz s "Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits." , ss.497 - 502, 2020. 10.14744/tjtes.2020.25260 |
| ISNAD | KURT OZKAYA, Nese vd. "Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits". (2020), 497-502. https://doi.org/10.14744/tjtes.2020.25260 |
| APA | KURT OZKAYA N, ZEREYAK U, Acikalin Coskun K, TUTAR Y, yılmaz s (2020). Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits. Ulusal Travma ve Acil Cerrahi Dergisi, 26(4), 497 - 502. 10.14744/tjtes.2020.25260 |
| Chicago | KURT OZKAYA Nese,ZEREYAK UMUT,Acikalin Coskun Kubra,TUTAR Yusuf,yılmaz sarper Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits. Ulusal Travma ve Acil Cerrahi Dergisi 26, no.4 (2020): 497 - 502. 10.14744/tjtes.2020.25260 |
| MLA | KURT OZKAYA Nese,ZEREYAK UMUT,Acikalin Coskun Kubra,TUTAR Yusuf,yılmaz sarper Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits. Ulusal Travma ve Acil Cerrahi Dergisi, vol.26, no.4, 2020, ss.497 - 502. 10.14744/tjtes.2020.25260 |
| AMA | KURT OZKAYA N,ZEREYAK U,Acikalin Coskun K,TUTAR Y,yılmaz s Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits. Ulusal Travma ve Acil Cerrahi Dergisi. 2020; 26(4): 497 - 502. 10.14744/tjtes.2020.25260 |
| Vancouver | KURT OZKAYA N,ZEREYAK U,Acikalin Coskun K,TUTAR Y,yılmaz s Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits. Ulusal Travma ve Acil Cerrahi Dergisi. 2020; 26(4): 497 - 502. 10.14744/tjtes.2020.25260 |
| IEEE | KURT OZKAYA N,ZEREYAK U,Acikalin Coskun K,TUTAR Y,yılmaz s "Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits." Ulusal Travma ve Acil Cerrahi Dergisi, 26, ss.497 - 502, 2020. 10.14744/tjtes.2020.25260 |
| ISNAD | KURT OZKAYA, Nese vd. "Assessment of plasma and tissue fibronectin EIIIB splice variant expressions measured serially using RT-PCR in a wound model of rabbits". Ulusal Travma ve Acil Cerrahi Dergisi 26/4 (2020), 497-502. https://doi.org/10.14744/tjtes.2020.25260 |
