Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777)

ÜRKÜ, Çiğdem; AKAYLI, Tülay; YARDIMCI, Remziye Eda

Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777)

Tülay AKAYLI, (İstanbul Üniversitesi, Su Bilimleri Fakültesi, Su Ürünleri ve Balık Hastalıkları Anabilim Dalı, İstanbul, Türkiye)

Remziye Eda YARDIMCI, (İstanbul Üniversitesi, Su Bilimleri Fakültesi, Su Ürünleri ve Balık Hastalıkları Anabilim Dalı, İstanbul, Türkiye)

Çiğdem ÜRKÜ (İstanbul Üniversitesi, Su Bilimleri Fakültesi, Su Ürünleri ve Balık Hastalıkları Anabilim Dalı, İstanbul, Türkiye)

Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi

12 2

Yıl: 2020 Cilt: 36 Sayı: 2 Sayfa Aralığı: 214 - 220 Metin Dili: İngilizce İndeks Tarihi: 22-10-2020

Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777)

Öz:

The aim of this study was to determinate the causative agent of mortalities in sharpsnout sea bream (Diplodus puntazzo Cetti, 1777) cultured in the Aegean Sea. Ten individuals (200-350 g) in were investigated total between May 2009 – 2010 using bacteriological methods. Moribund fish samples exhibited pale gills and liver, skin depigmentation, hemorrhages on the liver and muscles.Bacteriological samples of liver, kidney and spleen were streaked onto Marine Agar (MA) and Tryptic Soy Agar (TSA) plates containing 1,5% NaCl and incubated at 22°Cfor 48h. Gram-positive non-motile cocci were isolated from visceral organs of diseased fish samples. According to their morphological and biochemical characteristics, these isolates were identified as Micrococcus luteus. Kirby-Bauerdisk diffusion method was also performed using multidisc. Results were interpreted based on the available data and all isolates were determined to be sensitive againstoxytetracycline, erythromycin and chloramphenicol. Histopathologically, vacuolar degeneration and necrosis in the parenchyma cells of liver, tubular necrosis in thekidney and hemosiderin deposite in the spleen were determined. In addition to melting lamella, epithelial cells hyperplasia in the gills was observed. In conclusion,in this study an epizootic with a low mortality rate caused by M. luteus was described and this study is the first report of the pathogen in the cultured sharpsnout seabream.

Anahtar Kelime:

Kültüre Edilen Sivriburun Karagöz Balıklarında (Diplodus puntazzo Cetti, 1777) Micrococcus luteus Enfeksiyonunun Teşhisi

Öz:

Bu çalışmanın amacı Ege Denizi'nde kültüre edilen sivriburun karagöz balıklarında (Diplodus puntazzo Cetti, 1777) meydana gelen ölümlerin nedeninin belirlenmesidir. Mayıs 2009 ve Mayıs 2010 tarihleri arasında bakteriyolojik yöntemler kullanılarak toplam 10 hasta birey (200-350g) incelendi. Ölmek üzere olan balık örneklerinde solgun solungaç ve karaciğer, deri renginde açılma, karaciğerde ve kaslarda kanamalar görüldü. Hasta balıkların karaciğer, böbrek ve dalağından Marine Agar (MA) ve % 1,5 NaCl içeren Tryptic Soy Agar’a (TSA) yapılan bakteriyolojik ekimler 22 ° C'de 48 saat süreyle inkübe edildi. Gram pozitif hareketli olmayan koklar viseral organlardan izole edildi. Morfolojik ve biyokimyasal özelliklerine göre, bu izolatlar Miccrococcus luteus olarak identifiye edildi. KirbyBauer disk difüzyon yöntemi çoklu disk kullanılarak yapıldı. Sonuçlar mevcut verilerine dayanılarak yorumlandı ve tüm izolatların oksitetrasiklin, eritromisin ve kloramfenikole karşı duyarlı olduğu belirlendi. Histopatolojik olarak karaciğer parankim hücrelerinde vakuolar dejenerasyon ve nekroz, böbrekte tübüler nekroz ve dalakta hemosiderin depozitleri saptandı. Lamellalarda erimenin yanı sıra solungaçlarda epitel hücre hiperplazisi gözlendi. Sonuç olarak, bu çalışmada M. luteus’un neden olduğu düşük mortaliteli bir epizootik tanımlandı ve bu çalışma bu patojenin kültür sivriburun karagöz balıklarında tanımlandığı ilk rapordur.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	AKAYLI T, YARDIMCI R, ÜRKÜ Ç (2020). Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777). , 214 - 220.
Chicago	AKAYLI Tülay,YARDIMCI Remziye Eda,ÜRKÜ Çiğdem Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777). (2020): 214 - 220.
MLA	AKAYLI Tülay,YARDIMCI Remziye Eda,ÜRKÜ Çiğdem Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777). , 2020, ss.214 - 220.
AMA	AKAYLI T,YARDIMCI R,ÜRKÜ Ç Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777). . 2020; 214 - 220.
Vancouver	AKAYLI T,YARDIMCI R,ÜRKÜ Ç Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777). . 2020; 214 - 220.
IEEE	AKAYLI T,YARDIMCI R,ÜRKÜ Ç "Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777)." , ss.214 - 220, 2020.
ISNAD	AKAYLI, Tülay vd. "Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777)". (2020), 214-220.

APA	AKAYLI T, YARDIMCI R, ÜRKÜ Ç (2020). Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777). Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 36(2), 214 - 220.
Chicago	AKAYLI Tülay,YARDIMCI Remziye Eda,ÜRKÜ Çiğdem Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777). Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi 36, no.2 (2020): 214 - 220.
MLA	AKAYLI Tülay,YARDIMCI Remziye Eda,ÜRKÜ Çiğdem Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777). Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.36, no.2, 2020, ss.214 - 220.
AMA	AKAYLI T,YARDIMCI R,ÜRKÜ Ç Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777). Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2020; 36(2): 214 - 220.
Vancouver	AKAYLI T,YARDIMCI R,ÜRKÜ Ç Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777). Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2020; 36(2): 214 - 220.
IEEE	AKAYLI T,YARDIMCI R,ÜRKÜ Ç "Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777)." Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 36, ss.214 - 220, 2020.
ISNAD	AKAYLI, Tülay vd. "Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777)". Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi 36/2 (2020), 214-220.