Objective: We planned and conducted this study to determine the knowledge levels of midwifery students for the non-pharmacological methods used in the management of labor pain and to raise awareness on this topic. Methods: This descriptive study was conducted with 60 students who attend the Department of Midwifery, Health College, ‹stanbul Bilim University and accepted to participate in the research. The data were collected by the questionnaire forms upon obtaining the approvals of the institution and the ethics committee. The questionnaires were assessed by SPSS version 22 (SPSS Inc., Chicago, IL, USA). Results: We found that 43.3% (n=26) of the midwifery students have knowledge on the non-pharmacological methods and apply at least one of these methods to the pregnant women. When the students were compared according to their grades, it was found that the total scores of responses given by students in 2nd grade for the questions to determine their attitudes for the non-pharmacological methods used in the management of labor pain were higher in a statistically significant way than the scores of students in 4th grade, and when they were compared in terms of their ages, it was seen that the total scores of the responses given by the students who are 1–19, 22–23 and 24 years old and above for the questions to determine their behaviors were higher in a statistically significant way than the students who are 20–21 years old. Conclusion: It is needed to increase the knowledge level of the students on the non-pharmacological methods used in the management of labor pain. In order to do this, the knowledge level of the students should be reinforced by using visual learning techniques and simulation practice methods in addition to their theoretical education.