Aim: Hysterectomy is one of the most prevalent surgical intervention in gynecological field. Thisstudy concerns the requirement and necessity of an endometrial sampling to exclude an unpredictableuterine malignancy risk before hysterectomy for benign conditions.Methods: This is a retrospective and cross-sectional study involving 307 patients who hadhysterectomy for benign conditions between years 2014-2018. Prior to hysterectomy, in 162 cases,an endometrial sampling was performed ahead (biopsy group) and in 145 cases, hysterectomy wasperformed without obtaining an endometrial sampling earlier on (control group). Pre- and postsurgical pathological evaluation reports were collected and compared between the groups.Results: Features of the patients as mean age, number of previous pregnancies, being atpostmenopausal status did not vary significantly between two groups. In the biopsy group, 51.2% ofthe patients were in the premenopausal period, 40.7% of the patients had postmenopausal bleedingand 52.5% had abnormal uterine bleeding symptoms and these three features were significantlyhigher than in the control group. In the control group, one case (0.7%) had leiomyosarcoma andanother case (0.7%) had endometrial adenocarcinoma. In the biopsy group, endometrialadenocarcinoma was detected in 5 patients (3.1%) as significantly higher than in the control group.Conclusion: If there are no symptoms, clinical and radiological findings suggesting an endometrialmalignancy in patients who planned to undergo a hysterectomy for benign conditions, it isunnecessary to perform a routine endometrial biopsy.