Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey
Yıl: 2020 Cilt: 17 Sayı: 1 Sayfa Aralığı: 32 - 38 Metin Dili: İngilizce DOI: 10.32707/ercivet.690618 İndeks Tarihi: 10-11-2020
Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey
Öz: This study was carried out to investigate the presence and distribution of Theileria, Babesia and Anaplasmaspecies in cattle and their ticks from Sivas province. A total of 314 EDTA-blood samples and 610 ticks were analysed.A part of 18S and 16SrRNA genes of Theileria/Babesia and Anaplasma species were amplified from the genomicDNAs extracted of the blood samples and tick pools by with polymerase chain reaction (PCR). A total of 14 probes (twocatchall, two genera and ten species-specific) were bound on a membrane and then the PCR products were tested byreverse line blot (RLB) assay. The partial sequences of the 18S and 16S rRNA genes of representative positive sampleswere determined. According to the results of the blood and tick samples analysed by RLB and sequencing, T.buffeli (GenBank accesion number: KJ183080), A. centrale (KJ183082), A. marginale (KJ183083), A. bovis(KJ183084), one Babesia genotype (Babesia sp. Sivas, KJ183081) and one Anaplasma genotype (Anaplasma sp.Sivas, KJ210855) were detected. Babesia sp. Sivas were found to be 99% identical with B. occultans, Babesia sp.Kashi and Babesia sp. Kayseri whereas Anaplasma sp. Sivas were found to be 99% and 98% identical with Anaplasmasp. Clone 7 and A. bovis, respectively. Overall prevalences of Theileria and Anaplasma infections in cattle werefound to be 5.10% and11.15% by RLB, respectively. This study is the first molecular survey on species of Theileria,Babesia and Anaplasma in cattle and ticks from Sivas.
Anahtar Kelime: Sivas Yöresinde Sığır ve Kenelerde Theileria, Babesia ve Anaplasma Enfeksiyonlarının Araştırılması
Öz: Bu çalışmada, Sivas yöresinde sığır ve kenelerde Theileria, Babesia ve Anaplasma türlerinin varlığı ve yaygınlığının araştırılması amaçlandı. Sığırlardan toplanan 314 EDTA’lı kan ve 610 kene örneği analiz edildi. Kan ve kenelerden elde edilen genomic DNA’lardan polimeraz zincir reaksiyonu (PZR) ile Theileria, Babesia ve Anaplasma türlerinin 18S ve 16S rRNA parsiyal gen bölgeleri amplifiye edildi. Bir membran üzerine toplam 14 prob (iki cathall/genel, iki soy ve on tür) bağlandı ve PZR ürünleri reverse line blotting (RLB) ile analiz edildi. Pozitif örneklerin 18S ve 16S rRNA genlerinin kısmi dizileri belirlendi. RLB ve sekans analizi ile Sivas yöresinde sığır ve kenelerde; T. buffeli (KJ183080), A. centrale (KJ183082), A. marginale (KJ183083), A. bovis (KJ183084) ve bir Babesia genotipi (Babesia sp. Sivas, KJ183081) ile bir Anaplasma genotipi (Anaplasma sp. Sivas, KJ210855) tespit edildi. Babesia sp. Sivas izolatının, B. occultans, Babesia sp. Kashi ve Babesia sp. Kayseri izolatları ile %99, Anaplasma sp. Sivas izolatının ise Anaplasma sp. Clone 7 ile %99 ve A. bovis ile %98 benzerliğe sahip olduğu belirlendi. Sığırlarda Theileria ve Anaplasma enfeksiyonlarının genel prevalansı, RLB'de sırasıyla %5.10 ve %11.15 olarak bulundu. Bu çalışma Sivas yöresinde sığırlarda ve kenelerde Theileria, Babesia ve Anaplasma türlerinin belirlendiği ilk moleküler çalışma niteliğindedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ALTAY K, ATAS A, OGRAK Y, ÖZKAN E (2020). Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey. , 32 - 38. 10.32707/ercivet.690618 |
| Chicago | ALTAY KÜRSAT,ATAS Ahmet Duran,OGRAK Yusuf Ziya,ÖZKAN Erkan Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey. (2020): 32 - 38. 10.32707/ercivet.690618 |
| MLA | ALTAY KÜRSAT,ATAS Ahmet Duran,OGRAK Yusuf Ziya,ÖZKAN Erkan Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey. , 2020, ss.32 - 38. 10.32707/ercivet.690618 |
| AMA | ALTAY K,ATAS A,OGRAK Y,ÖZKAN E Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey. . 2020; 32 - 38. 10.32707/ercivet.690618 |
| Vancouver | ALTAY K,ATAS A,OGRAK Y,ÖZKAN E Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey. . 2020; 32 - 38. 10.32707/ercivet.690618 |
| IEEE | ALTAY K,ATAS A,OGRAK Y,ÖZKAN E "Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey." , ss.32 - 38, 2020. 10.32707/ercivet.690618 |
| ISNAD | ALTAY, KÜRSAT vd. "Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey". (2020), 32-38. https://doi.org/10.32707/ercivet.690618 |
| APA | ALTAY K, ATAS A, OGRAK Y, ÖZKAN E (2020). Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 17(1), 32 - 38. 10.32707/ercivet.690618 |
| Chicago | ALTAY KÜRSAT,ATAS Ahmet Duran,OGRAK Yusuf Ziya,ÖZKAN Erkan Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 17, no.1 (2020): 32 - 38. 10.32707/ercivet.690618 |
| MLA | ALTAY KÜRSAT,ATAS Ahmet Duran,OGRAK Yusuf Ziya,ÖZKAN Erkan Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol.17, no.1, 2020, ss.32 - 38. 10.32707/ercivet.690618 |
| AMA | ALTAY K,ATAS A,OGRAK Y,ÖZKAN E Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2020; 17(1): 32 - 38. 10.32707/ercivet.690618 |
| Vancouver | ALTAY K,ATAS A,OGRAK Y,ÖZKAN E Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2020; 17(1): 32 - 38. 10.32707/ercivet.690618 |
| IEEE | ALTAY K,ATAS A,OGRAK Y,ÖZKAN E "Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey." Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 17, ss.32 - 38, 2020. 10.32707/ercivet.690618 |
| ISNAD | ALTAY, KÜRSAT vd. "Survey of Theileria, Babesia and Anaplasma Infections of Cattle and Ticks from Sivas Province of Turkey". Erciyes Üniversitesi Veteriner Fakültesi Dergisi 17/1 (2020), 32-38. https://doi.org/10.32707/ercivet.690618 |
