Objective: One of the most frequently observed painful procedures duringnewborn hospitalization is the neonatal heel prick. The use of vibrationtherapy in newborn pain control can be a preferred method for its non-invasive, cheap, and easy applicability. This study aimed to evaluate effectiveness of mechanical vibration application to avoid pain sensation duringheel puncture in newborns.Methods: This was a prospective single-center, placebo-controlled,, prospectiverandomized study. The study sample composed of healthy neonates born between 37 and 42 weeks of gestation in Medeniyet University Göztepe Trainingand Research Hospital. Sixty healthy neonates born in the study hospital between September 2012 and March 2013 and who matched the inclusion criteriaafter parental consent was obtained were included in the study. Heel puncturewas applied to the infants during routine testing for phenylketonuria. It was recorded that all of the newborns demographic characteristics, heart rate,oxygensaturation and body tempature measurement at the process. All applicationswas saved to video camera, then displayed followed and the NIPS was scoredby the investigator. The Cronbach’s alpha coefficient for the NIPS scoring systemwas 0.85 during the procedure and 0.87 after the procedure in this study.Results: Before procedure and during procedure the NIPS point averageof the experiment group is calculated by (0.967±1.771) and (1.733±2.050). For the control group, before and during application the NIPS point average is (3,567±1,775) and (4,533±1,907), respectively (p<0.05). In bothgroups Newborns post-procedure NIPS score results was higher thanduring procedure NIPS score and it was statistically significant (p<0.05).Conclusion: Mechanical vibration is found to be effective in decreasingpain sensation in neonates.