Yıl: 2018 Cilt: 8 Sayı: 3 Sayfa Aralığı: 196 - 201 Metin Dili: İngilizce DOI: 10.5152/clinexphealthsci.2017.658

Mast Cell Degranulation Mediates Compound 48/80-Induced Meningeal Vasodilatation Underlying Migraine Pain

Öz:
Objective: The cranial dura mater contains plenty of mast cells and is principallysupplied by the middle meningeal artery which has a key role in the generation ofheadaches. Neurogenic inflammation caused by perivascular nerve activation anddural vasodilation is held responsible for migraine pain. Dural mast cells contributeneurogenic inflammation and migraine via vasoactive and proinflammatory mediatorsin their secretory granules. In the present study, it was aimed to investigate vasoactiveeffect of mast cell degranulating agent compound 48/80 induced dural mast celldegranulation on the middle meningeal artery and its anterior and posterior branches.Methods: Isolated skulls obtained from male Wistar rats were divided into 2 halves.The skull cavities with intact the dura mater were applied synthetic interstitial fluidfor control group or mast cell degranulating agent compound 48/80 (10 µg/ml) insynthetic interstitial fluid for treated group at 37 oC for 15 min. Diameters of middlemeningeal artery and its anterior and posterior branches were measured and mastcells were counted from whole-mount preparations of meningeal dura mater.Results: While compound 48/80 induced massive degranulation of dural mast cells(P<0.01), it did not change the number of mast cells in the dura mater. Moreover,compound 48/80 increased diameter of middle meningeal artery (P<0.01) andits anterior (P<0.05) and posterior (P<0.01) branches, respectively compared tosynthetic interstitial fluid treatment.Conclusion: Dural mast cell degranulation causes dilatation of middle meningealartery which is involved in the pathophysiology of migraine, therefore testing of mastcell stabilizing agents in vivo models of migraine pain may promise hope for the nextbig things in the treatment of migraine headaches.
Anahtar Kelime:

Mast Hücre Degranülasyonu Compound 48/80 ile Tetiklenmiş Migren Ağrısının Altında Yatan Meningeal Vazodilatasyona Aracılık Ediyor

Öz:
Amaç: Kraniyal dura mater çok sayıda mast hücresi barındırmaktadır ve başlıca baş ağrılarının oluşumunda önemli bir role sahip olan middle meningeal arter tarafından beslenmektedir. Migren baş ağrısı oluşumunda, perivasküler sinir aktivasyonu ve dural vazodilatasyonun yol açtığı nörojenik enflamasyon sorumlu tutulmaktadır. Dural mast hücreleri sekretuar granüllerinde bulunan vazoaktif ve proinflamatuar mediyatörler aracılığıyla nörojenik enflamasyon ve migrene katkıda bulunmaktadır. Sunulan çalışmada bir mast hücre degranülatörü olan compound-48/80 ile oluşturulan dural mast hücre degranülasyonunun middle meningeal arter ve bunun anterior ve posterior dalları üzerine vazoaktif etkisinin araştırılması amaçlanmıştır. Yöntemler: Wistar erkek sıçanlardan elde edilen izole kranyumlar iki yarıya bölündü. Dura materi sağlam hemi-kranyumların boşluğuna kontrol grubu için 37 oC’ de yapay interstisyel sıvı ve çalışma grubu için bir mast hücre degranüle edici madde olan compound-48/80 (10 µg/ml) 15 dakika uygulandı. Middle meningeal arter ve bunun anterior ve posterior dallarının çapları ölçüldü ve meningeal dura mater preparasyonlarından mast hücreleri sayıldı. Bulgular: Compound-48/80 dural mast hücrelerinin kitlesel degranülasyonunu tetiklerken (P<0.01), dura materdeki mast hücre sayısını değiştirmedi. Ayrıca compound-48/80, yapay interstisyel sıvı uygulaması ile karşılaştırıldığında, sırasıyla middle meningeal arter (P<0.01) ve bunun anterior (P<0.05) ve posterior (P<0.01) dallarının çaplarını artırdı. Sonuç: Dural mast hücre degranülasyonu migren patofizyolojisi ile ilişkili olan middle meningeal arter dilatasyonuna neden olmaktadır böylece, mast hücre stabilizatörlerinin in vivo migren modellerinde test edilmesi migren baş ağrılarının tedavisinde bir sonraki adım için umut vaadedici olabilir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • [1] Moskowitz MA. The visceral organ brain Implications for the pathophysiology of vascular head pain. Neurology 1991; 41: 182-86.
  • [2] Wang X, Fang Y, Liang J, Yan M, Hu R, Pan X. 5-HT7 receptors are involved in neurogenic dural vasodilatation in an experimental model of migraine. J Mol Neurosci 2014; 54: 164-70.
  • [3] Messlinger K, Hanesch U, Baumgärtel M, Trost B, Schmidt RF. Innervation of the dura mater encephali of cat and rat: ultrastructure and calcitonin gene-related peptide-like and substance P-like immunoreactivity. Anat Embryol (Berl) 1993; 188: 219-37.
  • [4] Kilinc E, Guerrero-Toro C, Zakharov A, Vitale C, Gubert-Olive M, Koroleva K, et al. Serotonergic mechanisms of trigeminal meningeal nociception: Implications for migraine pain. Neuropharmacology 2017; 116: 160-73.
  • [5] Zakharov A, Vitale C, Kilinc E, Koroleva K, Fayuk D, Shelukhina I, et al. Hunting for origins of migraine pain: cluster analysis of spontaneous and capsaicin-induced firing in meningeal trigeminal nerve fibers. Front Cell Neurosci 2015; 9: 287.
  • [6] Olesen J, Burstein R, Ashina M, Tfelt-Hansen P. Origin of pain in migraine: evidence for peripheral sensitisation. Lancet Neurol 2009; 8: 679-90.
  • [7] Xanthos DN, Gaderer S, Drdla R, Nuro E, Abramova A, Ellmeier W, et al. Central nervous system mast cells in peripheral inflammatory nociception. Mol Pain 2011; 7: 42.
  • [8] Levy D, Burstein R, Kainz V, Jakubowski M, Strassman AM. Mast cell degranulation activates a pain pathway underlying migraine headache. Pain 2007; 130: 166-76.
  • [9] Macfarlane R. New concepts of vascular headache. Ann R Coll Surg Engl 1993;75: 225-28.
  • [10] Rozniecki JJ, Dimitriadou V, Lambracht-Hall M, Pang X, Theoharides TC. Morphological and functional demonstration of rat dura mater mast cell-neuron interactions in vitro and in vivo. Brain Res 1999; 849: 1-15.
  • [11] Dimitriadou V, Henry P, Brochet B, Mathiau P, Aubineau P. Cluster headache: ultrastructural evidence for mast cell degranulation and interaction with nerve fibers in the human temporal artery. Cephalalgia 1990; 10: 221-28.
  • [12] Wolff H, Marcussen R, Kunkle EC. Studies on headache; analysis of the contractile state of the cranial vascular tree in migraine. Trans Am Neurol Assoc 1947; 73: 14-7.
  • [13] Kilinc E. Purinergic mechanisms in the nervous system: the role of adenosine triphosphate in the migraine pathophysiology. Abant Med J 2016;5:132-52.
  • [14] Pietrobon D, Striessnig J. Neurobiology of migraine. Nat Rev Neurosci 2003;4: 386-98.
  • [15] Asghar MS, Hansen AE, Amin FM, van der Geest RJ, Koning Pv, Larsson HB, et al. Evidence for a vascular factor in migraine. Ann Neurol 2011; 69: 635-45.
  • [16] Dalessio DJ. The relationship of vasoactive substances to vascular permeability, and their role in migraine. Res Clin Stud Headache 1976; 4: 76-84.
  • [17] Moskowitz M. Neurogenic inflammation in the pathophysiology and treatment of migraine. Neurology 1993; 43: S16-20.
  • [18] Levy D. Meningeal mast cells, inflammation and migraine pain. Drug Dev Res 2007; 68: 412-18.
  • [19] Tore F, Reynier-Rebuffel AM, Tuncel N, Callebert J, Aubineau P. Effects of sepsis on mast cells in rat dura mater: influence of L-NAME and VIP. Br J Pharmacol 2001; 134: 1367-74.
  • [20] Rosa AC, Fantozzi R. The role of histamine in neurogenic inflammation. Br J Pharmacol 2013; 170: 38-45.
  • [21] Cannon KE, Chazot PL, Hann V, Shenton F, Hough LB, Rice FL. Immunohistochemical localization of histamine H3 receptors in rodent skin, dorsal root ganglia, superior cervical ganglia, and spinal cord: potential antinociceptive targets. Pain 2007; 129: 76-92.
  • [22] Zuo Y, Perkins NM, Tracey DJ, Geczy CL. Inflammation and hyperalgesia induced by nerve injury in the rat: a key role of mast cells. Pain 2003; 105: 467-79.
  • [23] Chatterjea D, Wetzel A, Mack M, Engblom C, Allen J, Mora-Solano C, et al. Mast cell degranulation mediates compound 48/80-induced hyperalgesia in mice. Biochem Biophys Res Commun. 2012; 425: 237-43.
  • [24] Coruzzi G, Adami M, Guaita E, de Esch IJ, Leurs R. Antiinflammatory and antinociceptive effects of the selective histamine H4-receptor antagonists JNJ7777120 and VUF6002 in a rat model of carrageenaninduced acute inflammation. Eur J Pharmacol 2007; 563: 240-44.
  • [25] Ottosson A, Edvinsson L. Release of histamine from dural mast cells by substance P and calcitonin gene-related peptide. Cephalalgia 1997;17:166-74.
  • [26] Split W, Szmidt M, Prusiński A, Rozniecki J. Effectiveness of ketotifen-a drug stabilizing mast cell membranes-in the treatment of chronic forms of Horton’s headache. Neurol Neurochir Pol 1984;18: 105-9.
  • [27] Tore F, Tuncel N. Mast cells: target and source of neuropeptides. Curr Pharm Des 2009;15: 3433-45.
  • [28] Kilinc E, Firat T, Tore F, Kiyan A, Kukner A, Tunçel N. Vasoactive Intestinal peptide modulates c-Fos activity in the trigeminal nucleus and dura mater mast cells in sympathectomized rats. J Neurosci Res 2015; 93: 644-50.
  • [29] Kilinc E, Dagistan Y, Kotan B, Cetinkaya A. Effects of Nigella sativa seeds and certain species of fungi extracts on number and activation of dural mast cells in rats. Physiol Int 2017; 104: 15-24.
APA KILINÇ E, DAGİSTAN Y, TÖRE F (2018). Mast Cell Degranulation Mediates Compound 48/80-Induced Meningeal Vasodilatation Underlying Migraine Pain. Clinical and Experimental Health Sciences, 8(3), 196 - 201. 10.5152/clinexphealthsci.2017.658
Chicago KILINÇ ERKAN,DAGİSTAN Yaşar,TÖRE Fatma Mast Cell Degranulation Mediates Compound 48/80-Induced Meningeal Vasodilatation Underlying Migraine Pain. Clinical and Experimental Health Sciences 8, no.3 (2018): 196 - 201. 10.5152/clinexphealthsci.2017.658
MLA KILINÇ ERKAN,DAGİSTAN Yaşar,TÖRE Fatma Mast Cell Degranulation Mediates Compound 48/80-Induced Meningeal Vasodilatation Underlying Migraine Pain. Clinical and Experimental Health Sciences, vol.8, no.3, 2018, ss.196 - 201. 10.5152/clinexphealthsci.2017.658
AMA KILINÇ E,DAGİSTAN Y,TÖRE F Mast Cell Degranulation Mediates Compound 48/80-Induced Meningeal Vasodilatation Underlying Migraine Pain. Clinical and Experimental Health Sciences. 2018; 8(3): 196 - 201. 10.5152/clinexphealthsci.2017.658
Vancouver KILINÇ E,DAGİSTAN Y,TÖRE F Mast Cell Degranulation Mediates Compound 48/80-Induced Meningeal Vasodilatation Underlying Migraine Pain. Clinical and Experimental Health Sciences. 2018; 8(3): 196 - 201. 10.5152/clinexphealthsci.2017.658
IEEE KILINÇ E,DAGİSTAN Y,TÖRE F "Mast Cell Degranulation Mediates Compound 48/80-Induced Meningeal Vasodilatation Underlying Migraine Pain." Clinical and Experimental Health Sciences, 8, ss.196 - 201, 2018. 10.5152/clinexphealthsci.2017.658
ISNAD KILINÇ, ERKAN vd. "Mast Cell Degranulation Mediates Compound 48/80-Induced Meningeal Vasodilatation Underlying Migraine Pain". Clinical and Experimental Health Sciences 8/3 (2018), 196-201. https://doi.org/10.5152/clinexphealthsci.2017.658