TY  - JOUR
TI  - Astragalus trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate
AB  - In this study, two grams of callus regenerated from stem and leaf explants of Astragalus trojanus Stev. were cultured in Woody Plant Medium (WPM) supplemented with 1 mg/L 2,4-D for four weeks and used as inoculum in order to investigate theeffects of working volume and media composition. The highest biomass was obtained in 250 mL flask with astragaloside IV(1.66 µg/mg) and cycloastragenol (0.19 µg/mg) accumulation. Different concentrations of sucrose and casein hydrolysate (1and 2 g/L) were also tested and the effect of pH was also investigated. Biomass accumulation cannot be enhanced, however,astragaloside IV and cycloastragenol content was ascended. The highest astragaloside IV (95.23 µg/mg) and cycloastragenol(5.93 mg/mg) accumulations were obtained at pH 6.8 and 2 g/L casein hydrolysate, respectively
AU  - GÜREL, Aynur
AU  - NARTOP, Pınar
AU  - AKGÜN, İsmail Hakkı
AU  - Bedir, Erdal
PY  - 2019
JO  - Hacettepe Journal of Biology and Chemistry
VL  - 47
IS  - 2
SN  - 1303-5002
SP  - 185
EP  - 192
DB  - TRDizin
UR  - http://search/yayin/detay/375541
ER  -