Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats

KURT, Nezahat; guler, mustafa can; Eraslan, Ersen; tanyeli, ayhan; akaras, nurhan

doi:10.9775/kvfd.2019.22396

Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats

Ayhan TANYELİ, (Atatürk Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Erzurum, Türkiye)

Ersen ERASLAN, (Yozgat Bozok Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Yozgat, Türkiye)

Mustafa Can GÜLER, (Atatürk Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Erzurum, Türkiye)

Nezahat KURT, (Atatürk Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Erzurum, Türkiye)

Nurhan AKARAS (Aksaray Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Aksaray, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

10 1

Yıl: 2020 Cilt: 26 Sayı: 1 Sayfa Aralığı: 89 - 96 Metin Dili: İngilizce DOI: 10.9775/kvfd.2019.22396 İndeks Tarihi: 15-11-2020

Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats

Öz:

Renal injury occurring as a result of renal ischemia-reperfusion may lead to renal failure or even death. The aim of this study is to investigatepossible protective effcts of Gossypin on tissue damage occurred due to ischemia-reperfusion in rat kidney tissue. A total of 48 male Wistaralbino rats were used in the study. These rats were randomly divided into 6 groups equally (n = 8). The created groups were control (C),sham (S), ischemia-reperfusion (I/R), I/R + DMSO, I/R + 400 μg/kg gossypin and I/R + 4 mg/kg gossypin. In the rats of sham group, the rightnephrectomy was performed. In the rats of other groups rather than sham, the left renal artery was clamped after performing the rightnephrectomy. Gossypin was administered intraperitoneally before the reperfusion. 24 h reperfusion was applied to the left renal after 1 hof ischemia. TNF-α, IL-1β, IL-6 and IL-10 levels were measured with spectrophotometric methods in the kidney tissues after the procedureswere completed. Apoptosis and inflmmatory pathways were evaluated histopathologically using Caspase 3 and NF-κB antibodies. Therewas a statistically signifiant decrease in IL-1β and IL-6 levels of the gossypin groups compared to the I/R group (P<0.05). As the level of TNF-αwas decreased in the gossypin administered groups compared to the I/R group although not statistically signifiant, the level of IL-10 wasincreased. In the present study, we aimed to show that gossypin in renal I/R model is effctive on inflmmatory process and apoptosis andthat it can be used in routine treatment to decrease the damage in all reasons that may cause I/R. In addition, this study can shed light on thestudies to be done in this fild in the future.

Anahtar Kelime:

Gossypin Sıçanlarda Böbrek İskemi- Reperfüzyon Hasarına Karşı Korur

Öz:

Böbrek iskemi reperfüzyonu sonucu meydana gelen renal hasar, böbrek yetmezliğine ve hatta ölüme neden olabilir. Çalışmanın amacı sıçan böbrek dokusunda iskemi-reperfüzyona bağlı oluşan doku hasarına karşı gossypinin olası koruyucu etkilerini araştırmaktır. Çalışmada toplam 48 adet Wistar albino cinsi erkek sıçan kullanıldı. Sıçanlar randomize ve eşit olmak üzere 6 gruba ayrıldı (n=8). Kontrol (C), sham (S), istemi-reperfüzyon (I/R), I/R + DMSO, I/R + 400 μg/kg gossypin ve I/R + 4 mg/kg gossypin grupları oluşturuldu. Sham grubunda arka bölge açılarak sağ nefrektomi yapıldı. Gossypin reperfüzyondan önce intraperitoneal olarak uygulandı. Sol renal artere 1 saat iskemi sonunda 24 saat reperfüzyon uygulandı. Prosedürler tamamlandıktan sonra böbrek dokularında spektrofotometrik metodlarla TNF-α, IL- 1β, IL-6 and IL-10 seviyeleri ölçüldü. Apoptozis ve inflmatuvar yolaklar kazpaz-3 ve NF-κB antikorları kullanılarak değerlendirildi. Gossypin grupları I/R grupları ile kıyaslanınca IL-1β and IL-6 seviyelerinde istatistiksel olarak önemli derecede azalma tespit edildi (P<0.05). I/R grubu ile kıyaslandığında gossypin uygulanan gruplarda istatistiksel olarak anlamlı olmasa da TNF-α miktarlarına azalma tespit edildi. Mevcut çalışmada gossypinin I/R modelindeki inflmasyon aşaması ve apoptosis üzerine etkisini ve I/R neden olan tüm durumlarda oluşacak hasarı azaltmada kullanılabileceğini göstermeye çalıştık. Ek olarak, bu çalışma bulanda ileride yapılacak olan çalışmalara ışık tutabilir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	tanyeli a, Eraslan E, guler m, KURT N, akaras n (2020). Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats. , 89 - 96. 10.9775/kvfd.2019.22396
Chicago	tanyeli ayhan,Eraslan Ersen,guler mustafa can,KURT Nezahat,akaras nurhan Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats. (2020): 89 - 96. 10.9775/kvfd.2019.22396
MLA	tanyeli ayhan,Eraslan Ersen,guler mustafa can,KURT Nezahat,akaras nurhan Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats. , 2020, ss.89 - 96. 10.9775/kvfd.2019.22396
AMA	tanyeli a,Eraslan E,guler m,KURT N,akaras n Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats. . 2020; 89 - 96. 10.9775/kvfd.2019.22396
Vancouver	tanyeli a,Eraslan E,guler m,KURT N,akaras n Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats. . 2020; 89 - 96. 10.9775/kvfd.2019.22396
IEEE	tanyeli a,Eraslan E,guler m,KURT N,akaras n "Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats." , ss.89 - 96, 2020. 10.9775/kvfd.2019.22396
ISNAD	tanyeli, ayhan vd. "Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats". (2020), 89-96. https://doi.org/10.9775/kvfd.2019.22396

APA	tanyeli a, Eraslan E, guler m, KURT N, akaras n (2020). Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(1), 89 - 96. 10.9775/kvfd.2019.22396
Chicago	tanyeli ayhan,Eraslan Ersen,guler mustafa can,KURT Nezahat,akaras nurhan Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26, no.1 (2020): 89 - 96. 10.9775/kvfd.2019.22396
MLA	tanyeli ayhan,Eraslan Ersen,guler mustafa can,KURT Nezahat,akaras nurhan Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.26, no.1, 2020, ss.89 - 96. 10.9775/kvfd.2019.22396
AMA	tanyeli a,Eraslan E,guler m,KURT N,akaras n Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(1): 89 - 96. 10.9775/kvfd.2019.22396
Vancouver	tanyeli a,Eraslan E,guler m,KURT N,akaras n Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2020; 26(1): 89 - 96. 10.9775/kvfd.2019.22396
IEEE	tanyeli a,Eraslan E,guler m,KURT N,akaras n "Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26, ss.89 - 96, 2020. 10.9775/kvfd.2019.22396
ISNAD	tanyeli, ayhan vd. "Gossypin Protects Against Renal Ischemia- Reperfusion Injury in Rats". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 26/1 (2020), 89-96. https://doi.org/10.9775/kvfd.2019.22396