The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol

DEMİRCİ, Eşref; SONMEZ, MUSTAFA

The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol

Mustafa SÖNMEZ, (Fırat Üniversitesi, Veteriner Fakültesi, Dölerme ve Sun'i Tohumlama Anabilim Dalı, Elazığ, Türkiye)

Eşref DEMİRCİ (Girilmedi)

Turkish Journal of Veterinary and Animal Sciences

4 0

Yıl: 2004 Cilt: 28 Sayı: 5 Sayfa Aralığı: 893 - 899 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol

Öz:

Bu çalışma, farklı oranlarda gliserol içeren Tris-glikoz-yumurta sarısı sulandırıcısına sulandırılmış koç spermasının dondurulabiliriiği ve spermatolojik özellikleri üzerine askorbik asit'in etkisini araştırmak amacıyla yapılmıştır. Çalışmada altı Akkaraman koç kullanıldı. Sperma, elektroejakülatör yardımıyla 3 günde bir alındı. Alınan sperma örneklerinde spermatolojik özellikler incelendi. Pooling yapılan sperma örnekleri sulandırıldıktan sonra 2 saat içerisinde +4 °C'ye düşürüldü. Soğutulmuş sperma 20 eşit kısma ayrılıp, her bir kısım farklı oranlarda gliserol (%0, 1%, 3%, 5% and 7%) ve askorbik asit (0, 0,5, l, 2 mg/ml) içeren 20 farklı sulandırıcıdan biriyle 1:1 oranında sulandırıldı. Sulandırılmış sperma 0,25'lik Fransız payetlerinde 4 saat bekletildikten sonra sıvı azot buharında donduruldu. Dondurulan spermalar, +38 °C'de 25 saniyede çözdürüldü. Gruplar arasında, askorbik asit miktarının artışına bağlı olarak spermatolojik özelliklerde herhangi bir farklılık görülmedi. Gliserolizasyon ve ekilibrasyon sonrası, gliserol oranının artışına bağlı olarak akrozoma bağlı anormal spermatozoon, toplam anormal spermatozoon ve ölü spermatozoon oranlan yükselirken (P < 0,05), spermatozoon motilitesinin düştüğü (P < 0,05) görüldü. Spermanın dondurulup çözdürülmesi sonucu gliserol oranının artışına bağlı olarak akrozoma bağlı anormal spermatozoon oranı, toplam anormal spermatozoon oranı ve spermatozoon motilitesi yükselirken (P < 0,05) ölü spermatozoon oranı azaldı (P < 0,05). Sonuç olarak koç spermasında gliserolizasyon ve ekilibrasyon. sonrası, gliserol oranının artışı (%3-%7) spermatolojik özellikleri olumsuz yönde etkilemiş olmasına karşın dondurup çözdürme sonucu en iyi sonuçlar %5 gliserol katılan gruplardan elde edilmiştir. Dondurup çözdürme sonucu askorbik asit'in spermatolojik özellikler üzerine önemli bir etkisinin olmadığı bulunmuştur.

Anahtar Kelime: motilite spermatozoa semen gliserol koç askorbik asit semen sulandırıcısı dondurulabilirlik donma dondurarak koruma koyun çözülme Akkaraman

Konular: Ziraat Mühendisliği

Farklı oranlarda gliserol ilave edilensulandırıcılarla sulandırılan koç spermasının dondurulabilirliği üzerine askorbik asitin etkisi

Öz:

This study was conducted to investigate the effects of ascorbic acid on the freezability and spermatological characteristics of ram semen diluted with Tris-glucose-egg yolk based extenders containing different proportions of glycerol. Six Akkaraman rams were used. Semen was collected by electroejaculator once every 3 days. The spermatological characteristics of the collected semen samples were determined. Pooled and diluted semen was cooled slowly to + 4 °C over 2 h. Cooled semen was divided into 20 groups. Each of the 20 semen groups was diluted at a 1:1 ratio with one of the extender groups containing different proportions of glycerol (0%, 1%, 3%, 5%, and 7%) and ascorbic acid (0, 0.5, 1 and 2 mg/ml). Diluted semen was packaged in 0.25 ml French straws and equilibrated for 4 h before freezing. The semen in straws was frozen in liquid nitrogen vapor. The semen was thawed for 25 s at +38 °C. There was no significant difference in the spermatological characteristics depending on the increase in the proportions of acorbic acid after glycerolization-equilibration and freezing-thawing. While the increase in glycerol level in diluted semen reduced motility (P < 0.05), it raised the dead spermatozoa rate, and damaged the acrosome rate and total abnormal spermatozoa rate (P < 0.05) after glycerolization and equilibration. However, the increase in the glycerol level in diluted semen raised motility, and damaged the acrosome rate and total abnormal spermatozoa rate (P < 0.05), while it reduced the dead spermatozoa rate (P < 0.05) after freezing and thawing compared to the control diluent. In conclusion, the best results after freezing and thawing were observed in diluted semen groups containing 5% glycerol although the increase in the glycerol level (3%-7%) after glycerolization and equilibration in diluted semen had negative effects on spermatological characteristics. Moreover, the increase in the proportions of ascorbic acid in extended semen did not change any spermatoiogical characteristics.

Anahtar Kelime: freezability freezing cryopreservation sheep thawing Akkaraman motility spermatozoa semen glycerol rams ascorbic acid semen diluents

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SONMEZ M, DEMİRCİ E (2004). The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol. , 893 - 899.
Chicago	SONMEZ MUSTAFA,DEMİRCİ Eşref The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol. (2004): 893 - 899.
MLA	SONMEZ MUSTAFA,DEMİRCİ Eşref The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol. , 2004, ss.893 - 899.
AMA	SONMEZ M,DEMİRCİ E The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol. . 2004; 893 - 899.
Vancouver	SONMEZ M,DEMİRCİ E The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol. . 2004; 893 - 899.
IEEE	SONMEZ M,DEMİRCİ E "The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol." , ss.893 - 899, 2004.
ISNAD	SONMEZ, MUSTAFA - DEMİRCİ, Eşref. "The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol". (2004), 893-899.

APA	SONMEZ M, DEMİRCİ E (2004). The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol. Turkish Journal of Veterinary and Animal Sciences, 28(5), 893 - 899.
Chicago	SONMEZ MUSTAFA,DEMİRCİ Eşref The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol. Turkish Journal of Veterinary and Animal Sciences 28, no.5 (2004): 893 - 899.
MLA	SONMEZ MUSTAFA,DEMİRCİ Eşref The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol. Turkish Journal of Veterinary and Animal Sciences, vol.28, no.5, 2004, ss.893 - 899.
AMA	SONMEZ M,DEMİRCİ E The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol. Turkish Journal of Veterinary and Animal Sciences. 2004; 28(5): 893 - 899.
Vancouver	SONMEZ M,DEMİRCİ E The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol. Turkish Journal of Veterinary and Animal Sciences. 2004; 28(5): 893 - 899.
IEEE	SONMEZ M,DEMİRCİ E "The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol." Turkish Journal of Veterinary and Animal Sciences, 28, ss.893 - 899, 2004.
ISNAD	SONMEZ, MUSTAFA - DEMİRCİ, Eşref. "The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of glycerol". Turkish Journal of Veterinary and Animal Sciences 28/5 (2004), 893-899.