Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting

Gonenc, Bahadir; KIRCALI, Feride; SARIMEHMETOGLU, HIFSI OGUZ; KARA, MURAT

Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting

Bahadır GÖNENÇ, (Ankara Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Ankara, Türkiye)

Hıfsı Oğuz SARIMEHMETOĞLU, (Girilmedi)

Murat KARA, (Girilmedi)

Feride KIRCALI (Girilmedi)

Turkish Journal of Veterinary and Animal Sciences

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Yıl: 2004 Cilt: 28 Sayı: 5 Sayfa Aralığı: 943 - 949 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting

Öz:

Bu çalışmada, SDS-PAGE ve western blotting yöntemleri ile Fasciola hepatica ekskresyon/sekresyon (E/S) ve olgun antijenleri kullanılarak enfekte koyunlarda duyarlı ve özgün protein bantları tespit edilmiştir. Araştırmada, F. hepatica, Dicrocoelium dendriticum. Cyst hydatid, Cysticercus tenuicollis, Trichostrongylidae, Paramphistomum spp., ve Trichuris spp. ile doğal enfekte 20 koyun serumu ile aynı sayıda F. hepatica dışında diğer helmintlerle enfekte koyun serumları kullanılmıştır. Western blot yöntemi ile yapılan incelemede F. hepatica yönünden pozitif ve negatif gruplar arasındaki farklılıklar ortaya çıkarılmıştır. Araştırma sonuçlarına göre, E/S antijeni için 33, 39,5 ve 42 kDa, olgun antijeni için 24, 33, and 66 kDa'luk bantların spesifik olduğu belirlenmiştir.

Anahtar Kelime: SDS-PAGE bağışıksal tanı teşhis Fascioliasis Fasciola koyun western blotting antijenler Fasciola hepatica tanı teknikleri

Konular: Ziraat Mühendisliği

Koyunlarda Fasciola hepatica enfeksiyonunun ekskresyon/sekresyon ve olgun antijenleri kullanılarak Western Blotting yöntemi ile karşılaştırmalı teşhisi

Öz:

Crude and excretory/secretory (E/S) antigens of Fasciola hepatica were subjected to SDS-PAGE and Western blot analysis in order to identify protein bands that would enable the specific and sensitive immunodiagnosis of sheep. Sera from 20 sheep with natural infections of F. hepatica, Dicrocoelium dendriticum, Cyst hydatid, Cysticercus tenuicollis, Trichostrongylidae, Paramphistomum spp., and Trichuris spp. and the same number of sera naturally infected with the same helminths without F. hepatica were tested using Western blotting, and the bands obtained from positive and negative groups were compared. According to the test results the specific protein bands for F. hepatica infection were 33, 39.S and 42 kDa in E/S antigens and 24, 33 and 66 kDa in crude antigen.

Anahtar Kelime: Fasciola sheep western blotting antigens Fasciola hepatica diagnostic techniques SDS-PAGE immunodiagnosis diagnosis fascioliasis

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Gonenc B, SARIMEHMETOGLU H, KARA M, KIRCALI F (2004). Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting. , 943 - 949.
Chicago	Gonenc Bahadir,SARIMEHMETOGLU HIFSI OGUZ,KARA MURAT,KIRCALI Feride Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting. (2004): 943 - 949.
MLA	Gonenc Bahadir,SARIMEHMETOGLU HIFSI OGUZ,KARA MURAT,KIRCALI Feride Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting. , 2004, ss.943 - 949.
AMA	Gonenc B,SARIMEHMETOGLU H,KARA M,KIRCALI F Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting. . 2004; 943 - 949.
Vancouver	Gonenc B,SARIMEHMETOGLU H,KARA M,KIRCALI F Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting. . 2004; 943 - 949.
IEEE	Gonenc B,SARIMEHMETOGLU H,KARA M,KIRCALI F "Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting." , ss.943 - 949, 2004.
ISNAD	Gonenc, Bahadir vd. "Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting". (2004), 943-949.

APA	Gonenc B, SARIMEHMETOGLU H, KARA M, KIRCALI F (2004). Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting. Turkish Journal of Veterinary and Animal Sciences, 28(5), 943 - 949.
Chicago	Gonenc Bahadir,SARIMEHMETOGLU HIFSI OGUZ,KARA MURAT,KIRCALI Feride Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting. Turkish Journal of Veterinary and Animal Sciences 28, no.5 (2004): 943 - 949.
MLA	Gonenc Bahadir,SARIMEHMETOGLU HIFSI OGUZ,KARA MURAT,KIRCALI Feride Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting. Turkish Journal of Veterinary and Animal Sciences, vol.28, no.5, 2004, ss.943 - 949.
AMA	Gonenc B,SARIMEHMETOGLU H,KARA M,KIRCALI F Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting. Turkish Journal of Veterinary and Animal Sciences. 2004; 28(5): 943 - 949.
Vancouver	Gonenc B,SARIMEHMETOGLU H,KARA M,KIRCALI F Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting. Turkish Journal of Veterinary and Animal Sciences. 2004; 28(5): 943 - 949.
IEEE	Gonenc B,SARIMEHMETOGLU H,KARA M,KIRCALI F "Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting." Turkish Journal of Veterinary and Animal Sciences, 28, ss.943 - 949, 2004.
ISNAD	Gonenc, Bahadir vd. "Comparison of crude and excretory/Secretory antigens for the diagnosis of Fasciola hepatica in sheep by Western blotting". Turkish Journal of Veterinary and Animal Sciences 28/5 (2004), 943-949.