TY  - JOUR
TI  - Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures
AB  - Light is not only effective in photosynthesis, growthand development, but also play an important role inbiosynthesis of primary and secondary metabolites.The present research was aimed to determine effectof light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in cell suspension cultures of Echinacea purpurea L. Stem explants derivedfrom sterile plants were subjected to callus culture ina B5 media supplemented with 1.0 mg/L BAP and2.0 mg/L NAA. Afterward, 8-day cell cultures wereincubated in light and dark environments and cellharvesting, with a three-day interval, was carried outfive times. The contents of alkamide, caffeic acidderivatives and echinacoside were determined usingHPLC. Alkamide, caftaric acid and echinacoside content increased regularly with light application. Lightapplication increased alkamide content by 57%, asan average, compared to darkness. The cell culturesexposed to 12 days of incubation in light producedthe highest alkamide content, with a 2.11-fold increase, compared to dark. Subjecting the cell culturesto light for 12 days resulted in 70% and 63% increases in caftaric acid and echinacoside. This studyshowed that light application in Echinacea purpureaL. cell suspension culture had a great potential forincreasing some important phytochemicals.
AU  - BATI AY, Ebru
AU  - AÇIKGÖZ, Muhammed Akif
AU  - ODABAŞ, Sedanur
AU  - KARA, Şevket Metin
DO  - http://dx.doi.org/10.29278/azd.476349
PY  - 2018
JO  - Akademik Ziraat Dergisi
VL  - 7
IS  - 2
SN  - 2147-6403
SP  - 179
EP  - 184
DB  - TRDizin
UR  - http://search/yayin/detay/377493
ER  -