TY  - JOUR
TI  - Mesenchymal stem cells have significant anti-infective effect on methicillin-resistant Staphylococcus epidermidis vascular graft infections
AB  - Objectives: This study aims to evaluate the effects of mesenchymal stemcell (MSC) implantation on vascular graft infections caused by methicillinresistantStaphylococcus epidermidis (MRSE) and compare with antibiotictreatment.Materials and methods: Healthy adult 56 Wistar rats (age, over5 months; weighing, 300-350 g) were divided into eight groups. Group 1was defined as the control group and group 2 was defined as the infectedcontrol group. Groups 3 and 4 were defined as Dacron grafted and MRSEinfected groups, treated with tigecycline and MSCs, respectively. Groups 5and 6 were performed polytetrafluoroethylene (PTFE) graft and infectedwith MRSE. These groups were also administered tigecycline and MSCtreatment, respectively. Groups 7 and 8 were infected with MRSE withoutgraft administration and were also performed tigecycline and MSCtreatment, respectively. Grafts and soft tissue specimens were collected at13 days postoperatively. Colony counts of peri-graft tissue were performed.All samples were evaluated by enzyme-linked immunosorbent assay(ELISA) for the markers that determine stem cell activity.Results: The overall success of the treatments was assessed by thenumber of rats with MRSE recurrence, regardless of graft used. Thedifference between the untreated group 2, tigecycline groups (3, 5 and 7)and MSCs groups (4, 6 and 8) were statistically significant. Success of MSCand tigecycline treatments was similar in Dacron, PTFE, and non-graftedgroups. There was a resistance of MRSE infection in Dacron groups toMSC and tigecycline treatments. This was considered to be indicative ofthe susceptibility of the Dacron grafts to infection. However, there wasno significant difference between group 2 and Dacron groups in terms ofbacterial colonization. ELISA results were significant in three cytokines.Conclusion: Mesenchymal stem cells can be considered as an alternativetreatment option on its own or part of a combination therapy for control ofvascular graft infections.
AU  - TİRYAKİ, MERAL
AU  - Kabalcı, Mehmet
AU  - Cirpar, Meric
AU  - Canbeyli, Ibrahim Deniz
AU  - Oktas, Birhan
DO  - 10.5606/ehc.2019.66162
PY  - 2019
JO  - Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.)
VL  - 30
IS  - 3
SN  - 1305-8282
SP  - 201
EP  - 211
DB  - TRDizin
UR  - http://search/yayin/detay/378433
ER  -