EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION

ercan haydar, fadime güllü; Yıldırım, Çağdaş; Tanrıverdi, Fatih; Ozhasenekler, Ayhan; Yuzbasioglu, Yucel; Gökhan, Servan; eren, funda; Kahraman, Fatih Ahmet

doi:10.5505/amj.2020.71542

EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION

Fatih TANRIVERDİ, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Acil Tıp Ana Bilim Dalı, Ankara, Türkiye)

Yücel YÜZBAŞIOĞLU, (Keçiören Eğitim ve Araştırma Hastanesi, Acil Tıp Kliniği, Ankara, Türkiye)

Fadime Güllü ERCAN HAYDAR, (Ankara Şehir Hastanesi, Genel Cerrahi Kliniği, Ankara, Türkiye)

Şervan GÖKHAN, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Acil Tıp Ana Bilim Dalı, Ankara, Türkiye)

Ayhan ÖZHASENEKLER, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Acil Tıp Ana Bilim Dalı, Ankara, Türkiye)

Çağdaş YILDIRIM, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Acil Tıp Ana Bilim Dalı, Ankara, Türkiye)

Fatih KAHRAMAN, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Acil Tıp Ana Bilim Dalı, Ankara, Türkiye)

Funda EREN (Ankara Şehir Hastanesi, Biyokimya Bölümü, Ankara, Türkiye)

Ankara Medical Journal

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Yıl: 2020 Cilt: 20 Sayı: 1 Sayfa Aralığı: 47 - 56 Metin Dili: İngilizce DOI: 10.5505/amj.2020.71542 İndeks Tarihi: 28-11-2020

EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION

Öz:

Objectives: The aim of this study is to search if using thiol/disulphide homeostasis together with Neutrophile Lymphocyte Ratio (NLR) which are oxidative stress markers in patients who apply to the emergency service with carbon monoxide intoxication is useful regarding diagnosis or not. Materials and Methods: The study was performed prospectively with 45 patients who applied to the emergency service between 01.01.2014 - 01.03.2015 and who were included in the study and 40 healthy persons who were determined as control group. Thiol/disulphide homeostasis parameters (Thiol, disulphide, disulphide / native thiol, disulphide / total thiol, native thiol / total thiol) and NLR which are oxidative stress markers were studied in the patient and control groups by a method which was developed newly by Erel and Neşelioğlu. Results: In persons with carbon monoxide intoxication, a significant difference was detected in NT, TT, index 3 and NLR rates according to the control group. NT, TT and index 3 were found lower in the patient group, and NLR mean values were found higher in the patient group (p =0.005, < 0.001, = 0.032, < 0.001 respectively). Conclusion: To use NLR together with thiol/disulphide homeostasis parameters in the diagnosis of carbon monoxide intoxication is useful regarding diagnosing in these patients.

Anahtar Kelime:

KARBONMONOKSİT ZEHİRLENMESİNDE THİOL DİSULFİD HOMEOSTAZİSİ VE NÖTROFİL LENFOSİT ORANI DEĞERLENDİRİLMESİ

Öz:

Amaç: Bu çalışmanın amacı acil servise karbon monoksit zehirlenmesi nedeniyle başvuran hastalarda oksidatif stres markerlarından olan thiol/disülfit homeostazis parametreleri ile Nötrofil-Lenfosit (NLO) beraber kullanımının tanı açısından yararlı olup olmadığını araştırmaktır. Materyal ve Metot: Çalışma 01.01.2014 ile 01.03.2015 tarihleri arasında acil servise başvuran ve çalışmaya alınan 45 hasta ve kontrol grubu olarak belirlenen 40 sağlıklı kişi ile prospektif olarak yapıldı. Erel ve Neşelioğlu tarafından yeni geliştirilen bir metot ile oksidatif stres markerı olan thiol/disülfit homeostazis parametreleri (Thiol, disülfit, disülfit/native thiol, disülfit/total thiol, native thiol/total thiol) ile NLO hasta ve kontrol grubunda çalışıldı. Bulgular: Karbonmonoksit zehirlenmesi olanlarda kontrol grubuna göre, NT, TT, index 3 ve NLO oranlarında anlamlı farklılık tespit edildi. NT, TT ve index 3 hasta grubunda daha düşük, NLO ortalama değerleri daha yüksek bulundu (sırasıyla p =0,005; < 0,001; < 0,032 ve < 0,001). Sonuç: NLO ile thiol/disülfit homeostasis parametrelerinin karbonmonoksit zehirlenmesi tanısında beraber kullanılması bu hastalarda tanının konulması açısından faydalıdır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. J.A. Raub, M. Mathieu-Nolf, N.B. Hampson, S.R. Thom. Carbon monoxide poisoning a public health perspective. Toxicology 2000;145(1):1–14.
2. L.Wu, R.Wang. Carbonmonoxide: endogenousproduction physiologicalfunctions, and pharmacological applications. Pharmacol. Rev. 2005;57(4):585–630. http:// dx.doi.org/10.1124/pr.57.4.3.
3. G. Lippi, G. Rastelli, T. Meschi, L. Borghi, G. Cervellin. Pathophysiology, clinics, diagnosis and treatment of heart involvement in carbon monoxide poisoning. Clin. Biochem. 2012;45(16-17):1278–85. http://dx.doi.org/10.1016/j.clinbiochem. 2012.06.004.
4. Stanley T. Omaye, Metabolic Modulation of Carbon Monoxide Toxicity. Toxicology. 2002;180(2):139-50.
5. Raub JA, Mathieu-Nolf M, Hampson NB, Thom SR. Carbon Monoxide Poisoning- A Public Health Perspective. Toxicology 2000;145(1):1-14.
6. S. Akyol, S. Erdogan, N. Idiz, S. Celik, M. Kaya, F. Ucar, S. Dane, O. Akyol. The role of reactive oxygen species and oxidative stress in carbon monoxide toxicity: an in- depth analysis. Redox Rep 2014;19(5):180–9. http://dx.doi.org/10.1179/ 1351000214Y.0000000094.
7. T. Kalogeris, Y. Bao, R.J. Korthuis. Mitochondrial reactive oxygen species: a double edged sword in ischemia/reperfusion vs preconditioning. Redox Biol 2014;(2):702–14. http://dx.doi.org/10.1016/j.redox.2014.05.006.
8. A.M.Pisoschi, A.Pop. The role of antioxidants in the chemistry of oxidative stress:a review. Eur. J. Med. Chem 2015;97: 55–74. http://dx.doi.org/10.1016/j.ejmech. 2015.04.040.
9. O.Erel, S.Neselioglu. A novel and automated assay for thiol/disulphide homeostasis. Clin. Biochem 2014;47(18):326–32 http://dx.doi.org/10.1016/j.clinbiochem. 2014.09.026.
10. Lowsby R, Gomes C,Jarman I et al. Neutrophile to lymphocyte count ratio as an early indicator of blood stream infection in the emergency department. Emerg Med J 2015;32(7):531‐4
11. Bolat D, Topcu YF, Aydogdu O et al. Neutrophil to lymphocyte ratio as a predictor of early penile prosthesis implant infection. Int Urol Nephrol 2017;49(6):947‐53.
12. Bektas H. Vural G. Gumusyayla S. Deniz O. Alisik M. Erel O. Dynamic thiol-disulphide homeostasis in acute ischemic stroke patients. Acta Neurol Belg 2016 Dec;116(4):489-94.
13. Topuz M. Kaplan M. Akkus O. Sen O. Yunsel HD. Allahverdiyev S. Erel O. Koc M. Gur M. The prognostic importance of thiol/disulphide homeostasis in patients with acute pulmonary thromboembolism. Am J Emerg Med. 2016 Dec;34(12):2315-19.
14. Ergin M, Calıskantürk M, Senat A, Akturk O, Erel O. Disulphide stress in carbon monoxide poisoning. Clin Biochem. 2016 Nov;49(16-17):1243-47. doi: 10.1016/j.clinbiochem.2016.07.019. Epub 2016 Aug 3.
15. Kundi H, Erel Ö, Balun A, Çiçekçioglu H, Cetin M, Kiziltunç E et al. Association of thiol/disulphide ratio with syntax score in patients with NSTEMI. Scand Cardiovasc J 2015;49(2):95–100
16. Eren Y, Dirik E, Neselioglu S, Erel O. Oxidative stress and decreased thiol level in patients with migraine: cross-sectional study. Acta Neurol Belg 2015;115(49:643-9
17. Günaydın Y, Vural K, Ok M, Katırcı Y, KocaşabanDet.al. Comparison of carbon monoxide poisonings originated from coal stove and natural gas and the evaluation of Neutrophil/Lymphocyte ratio. Dicle Tip Dergsi 2015;42(3):299-304 doi: 10.5798/diclemedj.0921.2015.03.0578
18. S. Zengin, A B, S. Karta, B. Can, M. Orkmez, A. Taskin, U. Lok, B. Gulen, C. Yildirim, S. Taysi, An assessment of antioxidant status in patients with carbon monoxide poi- soning, World J. Emerg. Med. 2014;5(2):91–5. http://dx.doi.org/10.5847/wjem. j.1920-8642.2014.02.002.
19. Karabacak M, Türkdoğan KA, Coşkun A, Akpınar O. Detection of neutrophil–lymphocyte ratio as a serum marker associated with inflammations by acute carbon monoxide poisoning. Journal of Acute Disease October 2015;4(4):305-8. https://doi.org/10.1016/j.joad.2015.06.009
20. Solak I, Cetinkaya CD, Gederet YT, Kozanhan B, Erel O, Eryilmaz MA. Effects of smoking on thiol/disulphide homeostasis. Eur Rev Med Pharmacol Sci. 2018 Apr;22;(8):2477-82. doi: 10.26355/eurrev_201804_14842.

APA	Tanrıverdi F, Yuzbasioglu Y, ercan haydar f, Gökhan S, Ozhasenekler A, Yıldırım Ç, Kahraman F, eren f (2020). EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION. , 47 - 56. 10.5505/amj.2020.71542
Chicago	Tanrıverdi Fatih,Yuzbasioglu Yucel,ercan haydar fadime güllü,Gökhan Servan,Ozhasenekler Ayhan,Yıldırım Çağdaş,Kahraman Fatih Ahmet,eren funda EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION. (2020): 47 - 56. 10.5505/amj.2020.71542
MLA	Tanrıverdi Fatih,Yuzbasioglu Yucel,ercan haydar fadime güllü,Gökhan Servan,Ozhasenekler Ayhan,Yıldırım Çağdaş,Kahraman Fatih Ahmet,eren funda EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION. , 2020, ss.47 - 56. 10.5505/amj.2020.71542
AMA	Tanrıverdi F,Yuzbasioglu Y,ercan haydar f,Gökhan S,Ozhasenekler A,Yıldırım Ç,Kahraman F,eren f EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION. . 2020; 47 - 56. 10.5505/amj.2020.71542
Vancouver	Tanrıverdi F,Yuzbasioglu Y,ercan haydar f,Gökhan S,Ozhasenekler A,Yıldırım Ç,Kahraman F,eren f EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION. . 2020; 47 - 56. 10.5505/amj.2020.71542
IEEE	Tanrıverdi F,Yuzbasioglu Y,ercan haydar f,Gökhan S,Ozhasenekler A,Yıldırım Ç,Kahraman F,eren f "EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION." , ss.47 - 56, 2020. 10.5505/amj.2020.71542
ISNAD	Tanrıverdi, Fatih vd. "EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION". (2020), 47-56. https://doi.org/10.5505/amj.2020.71542

APA	Tanrıverdi F, Yuzbasioglu Y, ercan haydar f, Gökhan S, Ozhasenekler A, Yıldırım Ç, Kahraman F, eren f (2020). EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION. Ankara Medical Journal, 20(1), 47 - 56. 10.5505/amj.2020.71542
Chicago	Tanrıverdi Fatih,Yuzbasioglu Yucel,ercan haydar fadime güllü,Gökhan Servan,Ozhasenekler Ayhan,Yıldırım Çağdaş,Kahraman Fatih Ahmet,eren funda EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION. Ankara Medical Journal 20, no.1 (2020): 47 - 56. 10.5505/amj.2020.71542
MLA	Tanrıverdi Fatih,Yuzbasioglu Yucel,ercan haydar fadime güllü,Gökhan Servan,Ozhasenekler Ayhan,Yıldırım Çağdaş,Kahraman Fatih Ahmet,eren funda EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION. Ankara Medical Journal, vol.20, no.1, 2020, ss.47 - 56. 10.5505/amj.2020.71542
AMA	Tanrıverdi F,Yuzbasioglu Y,ercan haydar f,Gökhan S,Ozhasenekler A,Yıldırım Ç,Kahraman F,eren f EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION. Ankara Medical Journal. 2020; 20(1): 47 - 56. 10.5505/amj.2020.71542
Vancouver	Tanrıverdi F,Yuzbasioglu Y,ercan haydar f,Gökhan S,Ozhasenekler A,Yıldırım Ç,Kahraman F,eren f EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION. Ankara Medical Journal. 2020; 20(1): 47 - 56. 10.5505/amj.2020.71542
IEEE	Tanrıverdi F,Yuzbasioglu Y,ercan haydar f,Gökhan S,Ozhasenekler A,Yıldırım Ç,Kahraman F,eren f "EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION." Ankara Medical Journal, 20, ss.47 - 56, 2020. 10.5505/amj.2020.71542
ISNAD	Tanrıverdi, Fatih vd. "EVALUATION OF THIOL DISULPHIDE HOMEOSTASIS AND NEUTROPHILE LYMPHOCYTE RATIO IN CARBON MONOXIDE INTOXICATION". Ankara Medical Journal 20/1 (2020), 47-56. https://doi.org/10.5505/amj.2020.71542