TY  - JOUR
TI  - Correlation of HER2/TOP2A Gene Aberrations with RASSF1A/APC Gene Methylation Status in High-Risk Breast Cancer
AB  - OBJECTIVEBreast cancer (BC) is a heterogeneous malignancy and differs widely among different patients. The aimof this study was to investigate the relationship between the HER2/TOP2A gene aberrations and promoter methylation in RASSF1A/APC genes in patients with high-risk BC.METHODSFormalin-fixed paraffin embedded (FFPE) tissue samples from primary breast tumors (n=60) were assessed. HER2/TOP2A aberrations was evaluated using FISH method. DNA was extracted from FFPEtumor tissues, and Methylation-sensitive high resolution melting (MS-HRM) analysis were performedfor RASSF1A/APC genes methylation status.RESULTSHER2 amplification and TOP2A aberration were observed in 15/60 (25%) and 18/60 (30%) cases, respectively. According to the statistical analysis, HER2 amplification was associated with higher tumorgrade (p=0.001), PR status (p=0.025), and TOP2A aberrations (p=0.004). RASSF1A and APC methylation were 58/60 (96.6%) and 26/60 (43.3%), respectively. There was a significant correlation betweenAPC methylation and TOP2A aberration. APC gene methylation was significantly more frequent intumors with TOP2A aberration (p=0.026).CONCLUSIONOur results suggested that APC gene promoter hypermethylation was associated with TOP2A geneaberrations in patients with high-risk BC. This may be significant for targeted individual therapy. Additionally, it was confirmed that there was significant association of TOP2A gene aberrations with theHER2 gene amplification seen in BC.
AU  - EROĞLU, ONUR
AU  - ÇİFTCİ, Evrim
AU  - Cilingir, Oguz
AU  - ARTAN, Sevilhan
AU  - DURAK ARAS, Beyhan
AU  - NURSAL, Ayşe Feyda
AU  - baydemir, canan
DO  - 10.5505/tjo.2019.2017
PY  - 2020
JO  - Türk Onkoloji Dergisi
VL  - 35
IS  - 1
SN  - 1300-7467
SP  - 8
EP  - 14
DB  - TRDizin
UR  - http://search/yayin/detay/383019
ER  -