Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor

Nisari, Mustafa; Ertekin, Tolga; CEYLAN, Dilek; özdamar, saim; İNANÇ, Neriman; KAYMAK, EMIN

doi:10.14744/ejmi.2019.14740

Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor

Mustafa NİSARİ, (Nuh Naci Yazgan Üniversitesi, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü, Kayseri, Türkiye)

Emin KAYMAK, (Bozok Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Yozgat, Türkiye)

Tolga ERTEKİN, (Afyonkarahisar Sağlık Bilimleri Üniversitesi, Tıp Fakültesi, Anatomi Anabilim Dalı, Afyon, Türkiye)

Dilek CEYLAN, (Erciyes Üniversitesi, Genom ve Kök Hücre Merkezi, Kayseri, Türkiye)

Neriman İNANÇ, (Nuh Naci Yazgan Üniversitesi, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü, Kayseri, Türkiye)

Saim ÖZDAMAR (Pamukkale Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Denizli, Türkiye)

Eurasian Journal of Medical Investigation

4 0

Yıl: 2019 Cilt: 3 Sayı: 4 Sayfa Aralığı: 315 - 321 Metin Dili: İngilizce DOI: 10.14744/ejmi.2019.14740 İndeks Tarihi: 07-12-2020

Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor

Öz:

Objectives: Cancer is the second most common cause of death in the world. Several chemotherapeutic drugs havebeen studied for their anticancer features. Paclitaxel is one of these chemotherapeutic drugs with high medicinal interest.This study was conducted in order to investigate effects of paclitaxel on lipid peroxidation and antioxidant enzymesin tissues of mice bearing solid-form Ehrlich tumors.Methods: In this study, 36 Balb/C male mice aged 8-10 weeks and weighing 25-30 g were used. Six mice were kept ascancer stock to produce Ehrlich Ascites Tumor (EAT) cells. Thirty mice were distributed into three groups as healthy control,tumor control and paclitaxel treatment. 0.1 ml physiologic saline solution was administered into mice in healthycontrol subcutaneously (s.c.) for 15 days. The animals in tumor control and Paclitaxel treatment groups received 1x106EAT cells s.c. through nape skin on the first day of the experiment. After the application of EAT cells, 10 mg/kg Paclitaxelwas injected intraperitoneally on days 4, 9 and 14. At the end of the study (on day 15), animals were sacrificed and liver,kidney, brain and testis tissues were excised and analyzed for Malondialdehyde (MDA), by using TBARS method, superoxidedismutase (SOD) and catalase (CAT) activities spectrophotometrically.Results: Compared to the results of healthy control group, tumor increased kidney and liver MDA levels developmentslightly but not significantly. Paclitaxel treatment significantly reduced the increased MDA levels in kidney and liver(p<0.001). Paclitaxel had no effect on testis MDA but brain MDA level reduced with the help of EAT cell injection andPaclitaxel returned the brain MDA level close to the level of healthy control (p<0.001). EAT cell injection reduced catalaseactivity in kidney and liver (p<0.001) and Paclitaxel had no effect on catalase activities in these tissues. In EAT cellinjected mice; testis and brain catalase activities were higher than that of healthy control group that were returned tocontrol levels by Paclitaxel treatment. Paclitaxel had no significant effect on decreased kidney and liver SOD activitieswhereas it significantly reduced the increased SOD activities in testis (p<0.05) and in brain (p<0.01).Conclusion: Paclitaxel alleviated the lipid peroxidation in kidney and liver but had no effect on antioxidant status inthese tissues of solid-Ehrlich tumor-bearing mice.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Bibliyografik

1. T.C. Sağlık Bakanlığı (2015) Turkiye Kanser Kontrol Programı. T.C. Sağlık Bakanlığı Yayın No: 1. Ankara. http://kanser.gov.tr/ Dosya/NCCP_2013-2018.pdf. Accessed 30 May 2017.
2. T.C. Sağlık Bakanlığı, TürkiyeHalk Sağlığı Kurumu (2016) Türkiye kanser istatistikleri,Ankara. http://kanser.gov.tr/Dosya/ca_istatistik/ANA_rapor_2013v01_2.pdf. Accessed May 30 2017.; Cancer J Clin 2017; 67:7-30.
3. Siegel RL, Miller KD, Jemal A. Cancer statistics. CA Cancer J Clin 2017;67:7–30. [CrossRef ]
4. Kumar RS, Rajkapoor B, Perumal P, Dhanasekaran T, Jose MA, Jothimanivannan C. Antitumor activity of ProsopisglandulosaTorr on Ehrlich ascites carcinoma (EAC) tumor bearing mice. Iranian J Pharmaceut Res 2011;10:505–10.
5. Kabel AM. Effects of combination between methotrexate and histone deacetylase inhibitors on transplantable tumor model. Am J Med Stud 2014;2:12–8.
6. Jaganathan SK, Mondhe D, Wani ZA, Pal HC, Mandal M. Effect of honey and eugenol on Ehrlich ascites and solid carcinoma. J Biomed Biotechnol Accessed 22 August 2017.
7. Padmavathi R, Senthilnathan P, Chodon D, Sakthisekaran D. Therapeutic effect of paclitaxel and propolis on lipid peroxidation and antioxidant system in 7,12 dimethyl benz(a)anthracene- induced breast cancer in female Sprague Dawley rats. Life Sciences 2006;78:2820–5. [CrossRef ]
8. Vennila R, Thirunavukkarasu S V, Muthumary J. In-vivo studies on anticancer activity of taxol isolated from an endophytic Fungus pestalotiopsispaucisetasacc. VM1.Asian Journal of Pharmaceutical and Clinical Research 2012;4:30–4.
9. Wei Y, Ma L, Zhang L, XuX. Noncovalent interaction-assisted drug delivery system with highly efficient uptake and release of paclitaxel for anticancer therapy. Int J Nanomedicine 2017;12:7039–51. [CrossRef ]
10. Wang YF, Chen CYu, Chung YSF, Chiou H, Ren LoH. Involvement of oxidative stress and caspase activation in paclitaxel- induced apoptosis of primary effusion lymphoma cells. Cancer Chemother Pharmacol 2004;54:322–30. [CrossRef ]
11. Zang X, Wang G, Cai Q, Zheng X, Zhang J, et al. A Promising Microtubule Inhibitor Deoxypodophyllotoxin Exhibits Better Efficacy to Multidrug-Resistant Breast Cancer than Paclitaxel via Avoiding Efflux Transport. Drug Metab Dispos 2018;46:542–51.
12. Mohamed M. Abdel-Daim, Khaled Abo-EL-Sooud , Lotfi Aleya, Simona G. Bungu, Agnieszka Najda, and Rohit Saluja. Alleviation of Drugs and Chemicals Toxicity: Biomedical Value of Antioxidants Hindawi Oxidative Medicine and Cellular Longevity 2018;2.
13. Loft S, Poulsen HE. Cancer risk and oxidative DNA damage in man. J Mol Med 1996;74:297–312. [CrossRef ]
14. Abdel-Salam O M E, Youness ER, Hafez H F. The antioxidant status of the plasma in patients with breast cancer undergoing chemotherapy. Open Journal of Molecular and Integrative Physiology 2011;29–35. [CrossRef ]
15. Didžiapetrienė J, Bublevič J, Smailytė G, Kazbarienė B, Stukas R. Significance of blood serum catalase activity and malondialdehyde level for survival prognosis of ovarian cancer patients. Medicina 2014;50:204–8. [CrossRef ]
16. Sullivan LB, Chandel NS. Mitochondrial reactive oxygen species and cancer. Cancer Metabolism 2014;2:1–17. [CrossRef ]
17. Glasauer A, Chandel NS. Targeting antioxidants for cancer therapy. Biochem Pharmacol 2014;92:90–101. [CrossRef ]
18. Bakan E, Tays S, Polat MF, Dalga S, Umudum Z, Bakan N, et al. Nitric oxide levels and lipid peroxidation in plasma of patients with gastric cancer. JPn J Clin Oncol 2002;162–6. [CrossRef ]
19. Panis C, Herrera AC SA, VictorinoV J, Campos FC, Freitas LF, De RossiT, et al. Oxidative stress and hematological profiles of advanced breast cancer patients subjected to paclitaxel or doxorubicin chemotherapy. Breast Cancer Res Treat 2012;133: 89–97.
20. El-DeebM M K, El-SheredyH G, MohammedA F. The Role of Serum Trace Elements and Oxidative Stress in Egyptian Breast Cancer Patients. Advances in Breast Cancer Research 2016;5:37–47.
21. Radenkovic S, Milosevic Z, Konjevic G, Karadzic K, Rovcanin B, Buta M, et al. Lactate dehydrogenase, Catalase, and Superoxide dismutase in tumor tissue of breast cancer patients in respect to mammographic findings. Cell Biochem Biophys 2013;66:287–95. [CrossRef ]
22. Campos FC, Victorino VJ, Martins-Pinge MC, Cecchini AL, Panis C, Cecchini R. Systemic toxicity induced by paclitaxel in vivo is associated with the solvent cremophor EL through oxidative stress-driven mechanisms. Food ChemToxicol 2014;68:78–86.
23. Hadzic T, Aykin-Burns N, Zhu Y, Coleman MC, Leick K, Jacobson GM, et al. Paclitaxel combined with inhibitors of glucose and hydroperoxide metabolism enhances breast cancer cell killing via H 2-mediated oxidative stress. FreeRadic Biol Med 2010;15:48:1024–33. [CrossRef ]
24. Cosan D, Basaran A, Degirmenci I, Gunes HV, Aral E. The effect of paclitaxel on rats following benzo(a)pyrene treatment. Saudi Med J 2008;29:657–61.
25. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351–8. [CrossRef ]
26. Sun Y, Oberley LW, Elwell JH, Sierra‑Rivera E. Antioxidant enzyme activities in normal and transformed mouse liver cells. Int J Cancer 1989;44:1028–33. [CrossRef ]
27. Aebi H. Catalase. In: Burgmeyer HU, editor. Methods of Enzymatic Analysis. New York: Academic Press Publisher 1983;273.
28. Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: How they are linked? Free Radic Boil Med 2010;49:1603–16. [CrossRef ]
29. Kasapović J, Pejić S, Todorović A, Stojiljković v, Pajović SB. Antioxidant status and lipid peroxidation in the blood of breast cancer patients of different ages. Cell Biochem Funct 2008;26:723–30. [CrossRef ]
30. Glorieux C, Dejeans N, Sid B, Beck R, Calderon P B, Verrax J. Catalase overexpression in mammary cancer cells leads to a less aggressive phenotype and an altered response to chemotherapy. Biochemical Pharmacology 2011;82:1384–90. [CrossRef ]
31. Salem ML, Shoukry NM, Teleb WK, Abdel‑Daim MM and Abdel‑Rahman MA. In vitro and in vivo antitumor effects of the Egyptian scorpion Androctonus amoreuxi venom in an Ehrlich ascites tumor model. Springer Plus 2016;5:570. [CrossRef ]

APA	Nisari M, KAYMAK E, Ertekin T, CEYLAN D, İNANÇ N, özdamar s (2019). Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor. , 315 - 321. 10.14744/ejmi.2019.14740
Chicago	Nisari Mustafa,KAYMAK EMIN,Ertekin Tolga,CEYLAN Dilek,İNANÇ Neriman,özdamar saim Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor. (2019): 315 - 321. 10.14744/ejmi.2019.14740
MLA	Nisari Mustafa,KAYMAK EMIN,Ertekin Tolga,CEYLAN Dilek,İNANÇ Neriman,özdamar saim Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor. , 2019, ss.315 - 321. 10.14744/ejmi.2019.14740
AMA	Nisari M,KAYMAK E,Ertekin T,CEYLAN D,İNANÇ N,özdamar s Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor. . 2019; 315 - 321. 10.14744/ejmi.2019.14740
Vancouver	Nisari M,KAYMAK E,Ertekin T,CEYLAN D,İNANÇ N,özdamar s Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor. . 2019; 315 - 321. 10.14744/ejmi.2019.14740
IEEE	Nisari M,KAYMAK E,Ertekin T,CEYLAN D,İNANÇ N,özdamar s "Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor." , ss.315 - 321, 2019. 10.14744/ejmi.2019.14740
ISNAD	Nisari, Mustafa vd. "Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor". (2019), 315-321. https://doi.org/10.14744/ejmi.2019.14740

APA	Nisari M, KAYMAK E, Ertekin T, CEYLAN D, İNANÇ N, özdamar s (2019). Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor. Eurasian Journal of Medical Investigation, 3(4), 315 - 321. 10.14744/ejmi.2019.14740
Chicago	Nisari Mustafa,KAYMAK EMIN,Ertekin Tolga,CEYLAN Dilek,İNANÇ Neriman,özdamar saim Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor. Eurasian Journal of Medical Investigation 3, no.4 (2019): 315 - 321. 10.14744/ejmi.2019.14740
MLA	Nisari Mustafa,KAYMAK EMIN,Ertekin Tolga,CEYLAN Dilek,İNANÇ Neriman,özdamar saim Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor. Eurasian Journal of Medical Investigation, vol.3, no.4, 2019, ss.315 - 321. 10.14744/ejmi.2019.14740
AMA	Nisari M,KAYMAK E,Ertekin T,CEYLAN D,İNANÇ N,özdamar s Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor. Eurasian Journal of Medical Investigation. 2019; 3(4): 315 - 321. 10.14744/ejmi.2019.14740
Vancouver	Nisari M,KAYMAK E,Ertekin T,CEYLAN D,İNANÇ N,özdamar s Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor. Eurasian Journal of Medical Investigation. 2019; 3(4): 315 - 321. 10.14744/ejmi.2019.14740
IEEE	Nisari M,KAYMAK E,Ertekin T,CEYLAN D,İNANÇ N,özdamar s "Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor." Eurasian Journal of Medical Investigation, 3, ss.315 - 321, 2019. 10.14744/ejmi.2019.14740
ISNAD	Nisari, Mustafa vd. "Effects of Paclitaxel on Lipid Peroxidation and Antioxidant Enzymes in Tissues of Mice Bearing Ehrlich Solid Tumor". Eurasian Journal of Medical Investigation 3/4 (2019), 315-321. https://doi.org/10.14744/ejmi.2019.14740