EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA

Muhammet CEYLAN, (İstanbul Ticaret Üniversitesi, Mühendislik Fakültesi, Mekatronik Mühendisliği Bölümü, İstanbul, Türkiye)
Shang you YANG, (Via Christi Hospital, Wichita, Kansas, USA)
Ramazan ASMATULU (Mechanical Engineering, Engineering Faculty, Wichita State University 1845 Fairmount, Wichita, Kansas, USA)
Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi b- Teorik Bilimler
1 0
Yıl: 2020 Cilt: 8 Sayı: 2 Sayfa Aralığı: 293 - 302 Metin Dili: İngilizce DOI: 10.20290/estubtdb.658998 İndeks Tarihi: 08-01-2021

EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA

Öz:
Polycaprolactone (PCL) nanofiber with constant plasmid DNA addition and with different concentration of gentamicindesigned and studied for their effect on cell viability and release rate. PCL nanofibers were fabricated using electrospinningmethod with plasmid DNA and gentamicin addition. The plasmid DNA used in PCL nanofiber were extracted from E. coli.The scanning electron microscopy (SEM) images show that the nano-scale fiber structures have an average diameter of 113.9nm. UV microplate reader confirmed the existence of plasmid DNA in the PCL nanofibers. Elisa reader study showed theaddition of gentamicin in the fibers. Cell viability tests indicated that PCL nanofibers with 10% gentamicin on a fibroblast cellshowed high cell viability, which is related to surface areas and pore size of the electrospun fibers besides to the interactionamong gentamicin, plasmid DNA and electrospun fiber matrix.
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APA Ceylan M, Yang S, Asmatulu D (2020). EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. , 293 - 302. 10.20290/estubtdb.658998
Chicago Ceylan Muhammet,Yang Shang-You,Asmatulu Dr. Ramazan EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. (2020): 293 - 302. 10.20290/estubtdb.658998
MLA Ceylan Muhammet,Yang Shang-You,Asmatulu Dr. Ramazan EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. , 2020, ss.293 - 302. 10.20290/estubtdb.658998
AMA Ceylan M,Yang S,Asmatulu D EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. . 2020; 293 - 302. 10.20290/estubtdb.658998
Vancouver Ceylan M,Yang S,Asmatulu D EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. . 2020; 293 - 302. 10.20290/estubtdb.658998
IEEE Ceylan M,Yang S,Asmatulu D "EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA." , ss.293 - 302, 2020. 10.20290/estubtdb.658998
ISNAD Ceylan, Muhammet vd. "EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA". (2020), 293-302. https://doi.org/10.20290/estubtdb.658998
APA Ceylan M, Yang S, Asmatulu D (2020). EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi b- Teorik Bilimler, 8(2), 293 - 302. 10.20290/estubtdb.658998
Chicago Ceylan Muhammet,Yang Shang-You,Asmatulu Dr. Ramazan EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi b- Teorik Bilimler 8, no.2 (2020): 293 - 302. 10.20290/estubtdb.658998
MLA Ceylan Muhammet,Yang Shang-You,Asmatulu Dr. Ramazan EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi b- Teorik Bilimler, vol.8, no.2, 2020, ss.293 - 302. 10.20290/estubtdb.658998
AMA Ceylan M,Yang S,Asmatulu D EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi b- Teorik Bilimler. 2020; 8(2): 293 - 302. 10.20290/estubtdb.658998
Vancouver Ceylan M,Yang S,Asmatulu D EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi b- Teorik Bilimler. 2020; 8(2): 293 - 302. 10.20290/estubtdb.658998
IEEE Ceylan M,Yang S,Asmatulu D "EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA." Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi b- Teorik Bilimler, 8, ss.293 - 302, 2020. 10.20290/estubtdb.658998
ISNAD Ceylan, Muhammet vd. "EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA". Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi b- Teorik Bilimler 8/2 (2020), 293-302. https://doi.org/10.20290/estubtdb.658998
Sayfa Oluşturulma Tarihi
23-04-2024 16:27

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